Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain
The heterogeneous composition of tumors presents a significant obstacle to the selection of a single molecule as a potential universal inhibitor of tumor growth. Lipid signaling and cellular metabolism have become the main targets of anticancer treatment in recent years. The protein kinase C (PKC) r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biophysica |
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| Online Access: | https://www.mdpi.com/2673-4125/1/2/17 |
| _version_ | 1797524507536130048 |
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| author | Denis Horvath Silvia Tomkova Veronika Huntosova |
| author_facet | Denis Horvath Silvia Tomkova Veronika Huntosova |
| author_sort | Denis Horvath |
| collection | DOAJ |
| description | The heterogeneous composition of tumors presents a significant obstacle to the selection of a single molecule as a potential universal inhibitor of tumor growth. Lipid signaling and cellular metabolism have become the main targets of anticancer treatment in recent years. The protein kinase C (PKC) regulators Gö6976, rottlerin, hypericin, and phorbol myristyl acetate have been identified as agents affecting cellular metabolism. Measurable parameters describing metabolism, endocytosis, and respiration were subjected to a distance-based computational procedure for higher dimensions to complement and extend the knowledge gained from experimental data. The mutual distances of the parameters of the substances applied to the cancer cells in the presence and absence of lipids were calculated within the L<i>p</i> spaces. The distance-based methods and comparisons of the generalized distances suggested to us the exceptional role of hypericin in heterogeneous systems. Furthermore, our results are confirmed by Western blotting of the levels of respiratory chain proteins and enzymes active in oxidative stress defense in cancer cell monolayers and spheroids. PKCα and PKCδ have been studied for lipid-activated cell signaling. In this study, we attempt to apply the concept of parametric distance in cell signal transduction and activation where the above methods have not yet been used. |
| first_indexed | 2024-03-10T08:58:23Z |
| format | Article |
| id | doaj.art-5f10c3ecabec4d8fa8328f438fd9bc94 |
| institution | Directory Open Access Journal |
| issn | 2673-4125 |
| language | English |
| last_indexed | 2024-03-10T08:58:23Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biophysica |
| spelling | doaj.art-5f10c3ecabec4d8fa8328f438fd9bc942023-11-22T06:57:14ZengMDPI AGBiophysica2673-41252021-06-011222223710.3390/biophysica1020017Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory ChainDenis Horvath0Silvia Tomkova1Veronika Huntosova2Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaDepartment of Biophysics, Institute of Physics, Faculty of Science, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaCenter for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaThe heterogeneous composition of tumors presents a significant obstacle to the selection of a single molecule as a potential universal inhibitor of tumor growth. Lipid signaling and cellular metabolism have become the main targets of anticancer treatment in recent years. The protein kinase C (PKC) regulators Gö6976, rottlerin, hypericin, and phorbol myristyl acetate have been identified as agents affecting cellular metabolism. Measurable parameters describing metabolism, endocytosis, and respiration were subjected to a distance-based computational procedure for higher dimensions to complement and extend the knowledge gained from experimental data. The mutual distances of the parameters of the substances applied to the cancer cells in the presence and absence of lipids were calculated within the L<i>p</i> spaces. The distance-based methods and comparisons of the generalized distances suggested to us the exceptional role of hypericin in heterogeneous systems. Furthermore, our results are confirmed by Western blotting of the levels of respiratory chain proteins and enzymes active in oxidative stress defense in cancer cell monolayers and spheroids. PKCα and PKCδ have been studied for lipid-activated cell signaling. In this study, we attempt to apply the concept of parametric distance in cell signal transduction and activation where the above methods have not yet been used.https://www.mdpi.com/2673-4125/1/2/17anticancer drug identificationprotein kinase Cmetabolismoxidative phosphorylationoxidative stress defensehypericin |
| spellingShingle | Denis Horvath Silvia Tomkova Veronika Huntosova Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain Biophysica anticancer drug identification protein kinase C metabolism oxidative phosphorylation oxidative stress defense hypericin |
| title | Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain |
| title_full | Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain |
| title_fullStr | Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain |
| title_full_unstemmed | Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain |
| title_short | Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain |
| title_sort | selection of unique molecules for cancer treatment by distance based method hypericin effect on respiratory chain |
| topic | anticancer drug identification protein kinase C metabolism oxidative phosphorylation oxidative stress defense hypericin |
| url | https://www.mdpi.com/2673-4125/1/2/17 |
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