Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain
African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia. Genetically modified ASFVs containing deletions of virulence-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/7/599 |
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| author | Elizabeth Ramirez-Medina Elizabeth Vuono Vivian O’Donnell Lauren G. Holinka Ediane Silva Ayushi Rai Sarah Pruitt Consuelo Carrillo Douglas P. Gladue Manuel V. Borca |
| author_facet | Elizabeth Ramirez-Medina Elizabeth Vuono Vivian O’Donnell Lauren G. Holinka Ediane Silva Ayushi Rai Sarah Pruitt Consuelo Carrillo Douglas P. Gladue Manuel V. Borca |
| author_sort | Elizabeth Ramirez-Medina |
| collection | DOAJ |
| description | African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia. Genetically modified ASFVs containing deletions of virulence-associated genes have produced attenuated phenotypes and induced protective immunity in swine. Here we describe the differential behavior of two viral genes, NL (DP71L) and UK (DP96R), both originally described as being involved in virus virulence. Deletion of either of these genes efficiently attenuated ASFV strain E70. We demonstrated that deletion of the UK gene from the ASFV-G genome did not decrease virulence when compared to the parental virus. Conversely, deletion of the NL gene produced a heterogeneous response, with early death in one of the animals and transient fever in the other animals. With this knowledge, we attempted to increase the safety profile of the previously reported experimental vaccine ASFV-GΔ9GL/ΔUK by deleting the NL gene. A triple gene-deletion virus was produced, ASFV-GΔ9GL/ΔNL/ΔUK. Although ASFV-GΔ9GL/ΔNL/ΔUK replicated in primary cell cultures of swine macrophages, it demonstrated a severe replication deficiency in pigs, failing to induce protection against challenge with parental ASFV-G. |
| first_indexed | 2024-12-21T19:35:21Z |
| format | Article |
| id | doaj.art-5f10db02c1b345028b3a763214677c34 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T19:35:21Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-5f10db02c1b345028b3a763214677c342022-12-21T18:52:37ZengMDPI AGViruses1999-49152019-07-0111759910.3390/v11070599v11070599Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia StrainElizabeth Ramirez-Medina0Elizabeth Vuono1Vivian O’Donnell2Lauren G. Holinka3Ediane Silva4Ayushi Rai5Sarah Pruitt6Consuelo Carrillo7Douglas P. Gladue8Manuel V. Borca9Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAnimal and Plant Health Inspection, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAgricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USAAfrican swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs, African swine fever (ASF). The ASFV Georgia 2007 isolate (ASFV-G) is responsible for the current epidemic situation in Europe and Asia. Genetically modified ASFVs containing deletions of virulence-associated genes have produced attenuated phenotypes and induced protective immunity in swine. Here we describe the differential behavior of two viral genes, NL (DP71L) and UK (DP96R), both originally described as being involved in virus virulence. Deletion of either of these genes efficiently attenuated ASFV strain E70. We demonstrated that deletion of the UK gene from the ASFV-G genome did not decrease virulence when compared to the parental virus. Conversely, deletion of the NL gene produced a heterogeneous response, with early death in one of the animals and transient fever in the other animals. With this knowledge, we attempted to increase the safety profile of the previously reported experimental vaccine ASFV-GΔ9GL/ΔUK by deleting the NL gene. A triple gene-deletion virus was produced, ASFV-GΔ9GL/ΔNL/ΔUK. Although ASFV-GΔ9GL/ΔNL/ΔUK replicated in primary cell cultures of swine macrophages, it demonstrated a severe replication deficiency in pigs, failing to induce protection against challenge with parental ASFV-G.https://www.mdpi.com/1999-4915/11/7/599ASFVASFAfrican swine feverporcine |
| spellingShingle | Elizabeth Ramirez-Medina Elizabeth Vuono Vivian O’Donnell Lauren G. Holinka Ediane Silva Ayushi Rai Sarah Pruitt Consuelo Carrillo Douglas P. Gladue Manuel V. Borca Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain Viruses ASFV ASF African swine fever porcine |
| title | Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain |
| title_full | Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain |
| title_fullStr | Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain |
| title_full_unstemmed | Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain |
| title_short | Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain |
| title_sort | differential effect of the deletion of african swine fever virus virulence associated genes in the induction of attenuation of the highly virulent georgia strain |
| topic | ASFV ASF African swine fever porcine |
| url | https://www.mdpi.com/1999-4915/11/7/599 |
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