<i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety
<i>Brucella</i> RB51 is a live modified vaccine. Its use in water buffalo has been proposed using a vaccination protocol different to that used for cattle, but knowledge of the long-term effects of RB51 vaccination in this species remains incomplete. The aim of the study was to evaluate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/2078 |
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| author | Fabrizio De Massis Flavio Sacchini Nicola D’Alterio Giacomo Migliorati Nicola Ferri Emanuela Rossi Daniela Averaimo Antonio Petrini Michele Podaliri Vulpiani Fabrizia Perletta Diamante Rodomonti Mirella Luciani Giovanni Befacchia Marta Maggetti Tiziana Di Febo Chiara Di Pancrazio Ivanka Marinova Krasteva Romolo Salini Giacomo Vincifori Simona Iannetti Manuela Tittarelli |
| author_facet | Fabrizio De Massis Flavio Sacchini Nicola D’Alterio Giacomo Migliorati Nicola Ferri Emanuela Rossi Daniela Averaimo Antonio Petrini Michele Podaliri Vulpiani Fabrizia Perletta Diamante Rodomonti Mirella Luciani Giovanni Befacchia Marta Maggetti Tiziana Di Febo Chiara Di Pancrazio Ivanka Marinova Krasteva Romolo Salini Giacomo Vincifori Simona Iannetti Manuela Tittarelli |
| author_sort | Fabrizio De Massis |
| collection | DOAJ |
| description | <i>Brucella</i> RB51 is a live modified vaccine. Its use in water buffalo has been proposed using a vaccination protocol different to that used for cattle, but knowledge of the long-term effects of RB51 vaccination in this species remains incomplete. The aim of the study was to evaluate the safety and kinetics of antibody responses in water buffaloes vaccinated according to the protocol described for the bovine species in the WOAH Manual, modified with the use of a triple dose. Water buffaloes were vaccinated with the vaccine RB51. A booster vaccination was administered at 12 months of age. When turning 23–25 months old, female animals were induced to pregnancy. RB51-specific antibodies were detected and quantified using a CFT based on the RB51 antigen. Vaccinated animals showed a positive serological reaction following each vaccine injection, but titers and the duration of the antibody differed among animals. For 36 weeks after booster vaccination, the comparison of CFT values between vaccinated and control groups remained constantly significant. Afterwards, antibody titers decreased. No relevant changes in antibody response were recorded during pregnancy or lactation. In conclusion, results indicated that the vaccination schedule applied is safe and allows for vaccinated and unvaccinated controls to be discriminated between for up to 8 months after booster vaccination. |
| first_indexed | 2024-03-10T23:42:36Z |
| format | Article |
| id | doaj.art-5f14f18db9be497393ad066092826df0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:42:36Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5f14f18db9be497393ad066092826df02023-11-19T02:18:50ZengMDPI AGMicroorganisms2076-26072023-08-01118207810.3390/microorganisms11082078<i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine SafetyFabrizio De Massis0Flavio Sacchini1Nicola D’Alterio2Giacomo Migliorati3Nicola Ferri4Emanuela Rossi5Daniela Averaimo6Antonio Petrini7Michele Podaliri Vulpiani8Fabrizia Perletta9Diamante Rodomonti10Mirella Luciani11Giovanni Befacchia12Marta Maggetti13Tiziana Di Febo14Chiara Di Pancrazio15Ivanka Marinova Krasteva16Romolo Salini17Giacomo Vincifori18Simona Iannetti19Manuela Tittarelli20Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy<i>Brucella</i> RB51 is a live modified vaccine. Its use in water buffalo has been proposed using a vaccination protocol different to that used for cattle, but knowledge of the long-term effects of RB51 vaccination in this species remains incomplete. The aim of the study was to evaluate the safety and kinetics of antibody responses in water buffaloes vaccinated according to the protocol described for the bovine species in the WOAH Manual, modified with the use of a triple dose. Water buffaloes were vaccinated with the vaccine RB51. A booster vaccination was administered at 12 months of age. When turning 23–25 months old, female animals were induced to pregnancy. RB51-specific antibodies were detected and quantified using a CFT based on the RB51 antigen. Vaccinated animals showed a positive serological reaction following each vaccine injection, but titers and the duration of the antibody differed among animals. For 36 weeks after booster vaccination, the comparison of CFT values between vaccinated and control groups remained constantly significant. Afterwards, antibody titers decreased. No relevant changes in antibody response were recorded during pregnancy or lactation. In conclusion, results indicated that the vaccination schedule applied is safe and allows for vaccinated and unvaccinated controls to be discriminated between for up to 8 months after booster vaccination.https://www.mdpi.com/2076-2607/11/8/2078brucellosiswater buffalo<i>Brucella abortus</i> strain RB51vaccine safetyvaccinationcomplement fixation test |
| spellingShingle | Fabrizio De Massis Flavio Sacchini Nicola D’Alterio Giacomo Migliorati Nicola Ferri Emanuela Rossi Daniela Averaimo Antonio Petrini Michele Podaliri Vulpiani Fabrizia Perletta Diamante Rodomonti Mirella Luciani Giovanni Befacchia Marta Maggetti Tiziana Di Febo Chiara Di Pancrazio Ivanka Marinova Krasteva Romolo Salini Giacomo Vincifori Simona Iannetti Manuela Tittarelli <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety Microorganisms brucellosis water buffalo <i>Brucella abortus</i> strain RB51 vaccine safety vaccination complement fixation test |
| title | <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety |
| title_full | <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety |
| title_fullStr | <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety |
| title_full_unstemmed | <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety |
| title_short | <i>Brucella abortus</i> Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety |
| title_sort | i brucella abortus i strain rb51 administered to prepubescent water buffaloes from vaccination to lactation kinetics of antibody response and vaccine safety |
| topic | brucellosis water buffalo <i>Brucella abortus</i> strain RB51 vaccine safety vaccination complement fixation test |
| url | https://www.mdpi.com/2076-2607/11/8/2078 |
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