Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers
1-hydroxypyrene (1-OHP) is a metabolite of pyrene, which is a type of polycyclic aromatic hydrocarbons (PAHs) found in tobacco smoke and preferred use as a biomarker to determine exposure to PAHs from smoking. This paper reports a new and simple microextraction technique for 1-hydroxypyrene (1-OHP)...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2023-12-01
|
| Series: | Emerging Contaminants |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405665023000483 |
| _version_ | 1797615922815434752 |
|---|---|
| author | Nabil N. AL-Hashimi Husam Abed Alfattah Amjad H. El-Sheikh Jafar I. Abdelghani Abdelrahim M.A. Alqudah Alaa S. Alfawaz |
| author_facet | Nabil N. AL-Hashimi Husam Abed Alfattah Amjad H. El-Sheikh Jafar I. Abdelghani Abdelrahim M.A. Alqudah Alaa S. Alfawaz |
| author_sort | Nabil N. AL-Hashimi |
| collection | DOAJ |
| description | 1-hydroxypyrene (1-OHP) is a metabolite of pyrene, which is a type of polycyclic aromatic hydrocarbons (PAHs) found in tobacco smoke and preferred use as a biomarker to determine exposure to PAHs from smoking. This paper reports a new and simple microextraction technique for 1-hydroxypyrene (1-OHP) determination in smokers' urines using hydrophobic natural deep eutectic solvent (HNDES) in developing solvent bar microextraction (SBME) and high performance liquid chromatography-diode array detection (HPLC-DAD). Terpenoids based-HNDES using various ratios of (thymol/menthol) were synthesized and investigated as extraction solvents. The variables affecting HNDE-SBME steps were optimized by response surface methodology (RSM) based on central composite design (CCD). Under the optimum conditions, the calibration graph of spiked urine samples was linear in the range of 0.3–25.0 μg L−1 1-OHP with a correlation coefficient of 0.999. Additionally, the detection limit, quantitation limit, and extraction recovery were found to be 0.06 μg L−1, 0.22 μg L−1, and ≥100%, respectively. In comparison with referenced methods and based on the satisfactory results of applications, the proposed method can be considered as a potential method for the detection of urinary 1-OHP for health risk assessment of PAHs exposures. |
| first_indexed | 2024-03-11T07:33:52Z |
| format | Article |
| id | doaj.art-5f19cd0edf6445469aa7253ca6c58e58 |
| institution | Directory Open Access Journal |
| issn | 2405-6650 |
| language | English |
| last_indexed | 2024-03-11T07:33:52Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Emerging Contaminants |
| spelling | doaj.art-5f19cd0edf6445469aa7253ca6c58e582023-11-17T05:27:26ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502023-12-0194100250Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokersNabil N. AL-Hashimi0Husam Abed Alfattah1Amjad H. El-Sheikh2Jafar I. Abdelghani3Abdelrahim M.A. Alqudah4Alaa S. Alfawaz5The Hashemite University, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, P.O. Box 330127, Al-Zarqa, 13133, Jordan; Corresponding author.The Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa, 13115, JordanThe Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa, 13115, JordanThe Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa, 13115, JordanThe Hashemite University, Faculty of Pharmaceutical Sciences, Department of Clinical Pharmacy and Pharmacy Practice, P.O. Box 330127, Al-Zarqa, 13133, JordanThe Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa, 13115, Jordan1-hydroxypyrene (1-OHP) is a metabolite of pyrene, which is a type of polycyclic aromatic hydrocarbons (PAHs) found in tobacco smoke and preferred use as a biomarker to determine exposure to PAHs from smoking. This paper reports a new and simple microextraction technique for 1-hydroxypyrene (1-OHP) determination in smokers' urines using hydrophobic natural deep eutectic solvent (HNDES) in developing solvent bar microextraction (SBME) and high performance liquid chromatography-diode array detection (HPLC-DAD). Terpenoids based-HNDES using various ratios of (thymol/menthol) were synthesized and investigated as extraction solvents. The variables affecting HNDE-SBME steps were optimized by response surface methodology (RSM) based on central composite design (CCD). Under the optimum conditions, the calibration graph of spiked urine samples was linear in the range of 0.3–25.0 μg L−1 1-OHP with a correlation coefficient of 0.999. Additionally, the detection limit, quantitation limit, and extraction recovery were found to be 0.06 μg L−1, 0.22 μg L−1, and ≥100%, respectively. In comparison with referenced methods and based on the satisfactory results of applications, the proposed method can be considered as a potential method for the detection of urinary 1-OHP for health risk assessment of PAHs exposures.http://www.sciencedirect.com/science/article/pii/S2405665023000483Hydrophobic natural deep eutectic solventSolvent bar microextraction1-HydroxypyreneCentral composite designHPLC-DADUrine |
| spellingShingle | Nabil N. AL-Hashimi Husam Abed Alfattah Amjad H. El-Sheikh Jafar I. Abdelghani Abdelrahim M.A. Alqudah Alaa S. Alfawaz Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers Emerging Contaminants Hydrophobic natural deep eutectic solvent Solvent bar microextraction 1-Hydroxypyrene Central composite design HPLC-DAD Urine |
| title | Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers |
| title_full | Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers |
| title_fullStr | Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers |
| title_full_unstemmed | Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers |
| title_short | Hydrophobic natural deep eutectic-solvent bar microextraction combined with HPLC-DAD for determination of urinary 1-hydroxypyrene of smokers |
| title_sort | hydrophobic natural deep eutectic solvent bar microextraction combined with hplc dad for determination of urinary 1 hydroxypyrene of smokers |
| topic | Hydrophobic natural deep eutectic solvent Solvent bar microextraction 1-Hydroxypyrene Central composite design HPLC-DAD Urine |
| url | http://www.sciencedirect.com/science/article/pii/S2405665023000483 |
| work_keys_str_mv | AT nabilnalhashimi hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers AT husamabedalfattah hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers AT amjadhelsheikh hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers AT jafariabdelghani hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers AT abdelrahimmaalqudah hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers AT alaasalfawaz hydrophobicnaturaldeepeutecticsolventbarmicroextractioncombinedwithhplcdadfordeterminationofurinary1hydroxypyreneofsmokers |