Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities
The present research was performed to evaluate the bioactivity of Citrullus colocynthis (L.) and Melia azedarach L. extracts against three major stored grain insect pests including Tribolium castaneum (Herbst), Trogoderma granarium Everts, and Sitophilus granaries (L.). Toxicity and enzyme inhibitio...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2021-10-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-43356-en.html |
| _version_ | 1818212574435475456 |
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| author | Kazam Ali Muhammad Sagheer Mansoor ul Hasan Abdul Rashid Muhammad Shahid |
| author_facet | Kazam Ali Muhammad Sagheer Mansoor ul Hasan Abdul Rashid Muhammad Shahid |
| author_sort | Kazam Ali |
| collection | DOAJ |
| description | The present research was performed to evaluate the bioactivity of Citrullus colocynthis (L.) and Melia azedarach L. extracts against three major stored grain insect pests including Tribolium castaneum (Herbst), Trogoderma granarium Everts, and Sitophilus granaries (L.). Toxicity and enzyme inhibition activity of acetylcholinesterase (AChE), α-carboxylesterase (α-CE), β-carboxylesterase (β-CE), acid phosphatases (ACP) and alkaline phosphatases (ALP) in three insect species induced by both plant extracts were evaluated at four different dose rates viz., 5, 10, 15 and 20%. Results showed maximum mortality (34.29%) in S. granarius with M. azedarach at maximum interaction of time and dilution level. In T. castaneum and T. granarium maximum recorded values for mortality were 30.87% and 18.95%, respectively, with extract of M. azedarach. Plant extract of C. colocynthis reported a maximum mortality of 21.92%, 19.18% and 16.89% in T. castaneum, S. granarius and T. granarium, respectively. Findings proved that both plant extracts had decent lethal impacts on tested insect species. Exposure of studied insects to plants extracts also resulted in significant inhibition of AChE, α-CE, β-CE, ACP and ALP. All tested enzymes in three insects were maximally inhibited by plant extract of M. azedarach except α-CE which was slightly more inhibited in S. granarius and ACP which was highly inhibited in T. granarium and S. granarius, by plant extract of C. colocynthis. Outcomes exhibit that plant based extract of M. azedarach is more pronounced in stored grain insect pests and propose the capability of using these plant extracts for safety of stored commodities as a safe substitute for insecticides. |
| first_indexed | 2024-12-12T05:50:33Z |
| format | Article |
| id | doaj.art-5f1a8614fc5d4dea82d6f51fc06f810a |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-12-12T05:50:33Z |
| publishDate | 2021-10-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-5f1a8614fc5d4dea82d6f51fc06f810a2022-12-22T00:35:41ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2021-10-0110195109Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commoditiesKazam Ali0Muhammad Sagheer1Mansoor ul Hasan2Abdul Rashid3Muhammad Shahid4 Centre for Agriculture and Bioscience International, Central and West Asia, Rawalpindi, Pakistan. Department of Entomology, University of Agriculture, Faisalabad, Pakistan. Department of Entomology, University of Agriculture, Faisalabad, Pakistan. Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan. Department of Bio-Chemistry, University of Agriculture, Faisalabad, Pakistan. The present research was performed to evaluate the bioactivity of Citrullus colocynthis (L.) and Melia azedarach L. extracts against three major stored grain insect pests including Tribolium castaneum (Herbst), Trogoderma granarium Everts, and Sitophilus granaries (L.). Toxicity and enzyme inhibition activity of acetylcholinesterase (AChE), α-carboxylesterase (α-CE), β-carboxylesterase (β-CE), acid phosphatases (ACP) and alkaline phosphatases (ALP) in three insect species induced by both plant extracts were evaluated at four different dose rates viz., 5, 10, 15 and 20%. Results showed maximum mortality (34.29%) in S. granarius with M. azedarach at maximum interaction of time and dilution level. In T. castaneum and T. granarium maximum recorded values for mortality were 30.87% and 18.95%, respectively, with extract of M. azedarach. Plant extract of C. colocynthis reported a maximum mortality of 21.92%, 19.18% and 16.89% in T. castaneum, S. granarius and T. granarium, respectively. Findings proved that both plant extracts had decent lethal impacts on tested insect species. Exposure of studied insects to plants extracts also resulted in significant inhibition of AChE, α-CE, β-CE, ACP and ALP. All tested enzymes in three insects were maximally inhibited by plant extract of M. azedarach except α-CE which was slightly more inhibited in S. granarius and ACP which was highly inhibited in T. granarium and S. granarius, by plant extract of C. colocynthis. Outcomes exhibit that plant based extract of M. azedarach is more pronounced in stored grain insect pests and propose the capability of using these plant extracts for safety of stored commodities as a safe substitute for insecticides.http://jcp.modares.ac.ir/article-3-43356-en.htmlstored product pestsenzyme inhibitionlethal effectstoxicity |
| spellingShingle | Kazam Ali Muhammad Sagheer Mansoor ul Hasan Abdul Rashid Muhammad Shahid Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities Journal of Crop Protection stored product pests enzyme inhibition lethal effects toxicity |
| title | Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| title_full | Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| title_fullStr | Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| title_full_unstemmed | Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| title_short | Bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| title_sort | bioactivity of medicinal plant extracts as toxicants and enzyme inhibitors against insect pests of stored commodities |
| topic | stored product pests enzyme inhibition lethal effects toxicity |
| url | http://jcp.modares.ac.ir/article-3-43356-en.html |
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