Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth
Intraductal radiofrequency (RF) ablation combined with placement of a self-expandable metal stent (SEMS) for malignant biliary obstruction has risks such as stent- and heat-induced biliary sludge and restenosis. Here, we investigated the efficacy of a silver nanoparticles (AgNPs)-coated SEMS to inhi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/412 |
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| author | Yubeen Park Dong-Sung Won Ga-Hyun Bae Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Wooram Park Sang Soo Lee Jung-Hoon Park |
| author_facet | Yubeen Park Dong-Sung Won Ga-Hyun Bae Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Wooram Park Sang Soo Lee Jung-Hoon Park |
| author_sort | Yubeen Park |
| collection | DOAJ |
| description | Intraductal radiofrequency (RF) ablation combined with placement of a self-expandable metal stent (SEMS) for malignant biliary obstruction has risks such as stent- and heat-induced biliary sludge and restenosis. Here, we investigated the efficacy of a silver nanoparticles (AgNPs)-coated SEMS to inhibit tissue hyperplasia and bacterial growth caused by RF ablation with stent placement in the rabbit bile duct. The release behavior and antibacterial effects of AgNPs-coated SEMSs were evaluated. Then, SEMSs were successfully placed in all rabbits immediately after RF ablation. Ag ions were rapidly released at the beginning and then showed a gradual release behavior. The AgNPs-coated SEMS significantly inhibited bacterial activity compared to the uncoated SEMS (<i>p</i> < 0.05). Cholangiography and histological examination confirmed that the level of tissue hyperplasia was significantly lower in the AgNPs group than in the control group (all <i>p</i> < 0.05). Immunohistochemistry analyses revealed that TUNEL-, HSP 70-, and α-SMA-positive areas were significantly lower in the AgNPs group than in the control group (all <i>p</i> < 0.05). Intraductal RF ablation combined with nanofunctionalized stent placement represents a promising new approach for suppressing thermal damage as well as stent-induced tissue hyperplasia and bacterial growth. |
| first_indexed | 2024-03-09T21:13:56Z |
| format | Article |
| id | doaj.art-5f1b49ceefb04ad5b012b6be344e2634 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:13:56Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-5f1b49ceefb04ad5b012b6be344e26342023-11-23T21:38:55ZengMDPI AGPharmaceutics1999-49232022-02-0114241210.3390/pharmaceutics14020412Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial GrowthYubeen Park0Dong-Sung Won1Ga-Hyun Bae2Dae Sung Ryu3Jeon Min Kang4Ji Won Kim5Song Hee Kim6Chu Hui Zeng7Wooram Park8Sang Soo Lee9Jung-Hoon Park10Biomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaDepartment of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Bucheon 14662, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaDepartment of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Bucheon 14662, KoreaAsan Medical Center, Department of Gastroenterology, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaBiomedical Engineering Research Center, Asan Medical Center, Asan Institute for Life Science, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, KoreaIntraductal radiofrequency (RF) ablation combined with placement of a self-expandable metal stent (SEMS) for malignant biliary obstruction has risks such as stent- and heat-induced biliary sludge and restenosis. Here, we investigated the efficacy of a silver nanoparticles (AgNPs)-coated SEMS to inhibit tissue hyperplasia and bacterial growth caused by RF ablation with stent placement in the rabbit bile duct. The release behavior and antibacterial effects of AgNPs-coated SEMSs were evaluated. Then, SEMSs were successfully placed in all rabbits immediately after RF ablation. Ag ions were rapidly released at the beginning and then showed a gradual release behavior. The AgNPs-coated SEMS significantly inhibited bacterial activity compared to the uncoated SEMS (<i>p</i> < 0.05). Cholangiography and histological examination confirmed that the level of tissue hyperplasia was significantly lower in the AgNPs group than in the control group (all <i>p</i> < 0.05). Immunohistochemistry analyses revealed that TUNEL-, HSP 70-, and α-SMA-positive areas were significantly lower in the AgNPs group than in the control group (all <i>p</i> < 0.05). Intraductal RF ablation combined with nanofunctionalized stent placement represents a promising new approach for suppressing thermal damage as well as stent-induced tissue hyperplasia and bacterial growth.https://www.mdpi.com/1999-4923/14/2/412malignant biliary obstruction (MBO)radiofrequency (RF) ablationsilver nanoparticle (AgNP)self-expandable metal stent (SEMS)tissue hyperplasiaantibacterial effect |
| spellingShingle | Yubeen Park Dong-Sung Won Ga-Hyun Bae Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Wooram Park Sang Soo Lee Jung-Hoon Park Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth Pharmaceutics malignant biliary obstruction (MBO) radiofrequency (RF) ablation silver nanoparticle (AgNP) self-expandable metal stent (SEMS) tissue hyperplasia antibacterial effect |
| title | Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth |
| title_full | Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth |
| title_fullStr | Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth |
| title_full_unstemmed | Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth |
| title_short | Silver Nanofunctionalized Stent after Radiofrequency Ablation Suppresses Tissue Hyperplasia and Bacterial Growth |
| title_sort | silver nanofunctionalized stent after radiofrequency ablation suppresses tissue hyperplasia and bacterial growth |
| topic | malignant biliary obstruction (MBO) radiofrequency (RF) ablation silver nanoparticle (AgNP) self-expandable metal stent (SEMS) tissue hyperplasia antibacterial effect |
| url | https://www.mdpi.com/1999-4923/14/2/412 |
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