Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However,...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/10/3343 |
| _version_ | 1827667496430206976 |
|---|---|
| author | Viktoriia Korniienko Yevheniia Husak Julia Radwan-Pragłowska Viktoriia Holubnycha Yevhen Samokhin Anna Yanovska Julia Varava Kateryna Diedkova Łukasz Janus Maksym Pogorielov |
| author_facet | Viktoriia Korniienko Yevheniia Husak Julia Radwan-Pragłowska Viktoriia Holubnycha Yevhen Samokhin Anna Yanovska Julia Varava Kateryna Diedkova Łukasz Janus Maksym Pogorielov |
| author_sort | Viktoriia Korniienko |
| collection | DOAJ |
| description | Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both <i>E. coli</i> and <i>S. aureus</i> in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism. |
| first_indexed | 2024-03-10T03:18:21Z |
| format | Article |
| id | doaj.art-5f1bb8a874bf44ee9c0b5e0fbcfdb56e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:18:21Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5f1bb8a874bf44ee9c0b5e0fbcfdb56e2023-11-23T12:24:56ZengMDPI AGMolecules1420-30492022-05-012710334310.3390/molecules27103343Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan NanofibersViktoriia Korniienko0Yevheniia Husak1Julia Radwan-Pragłowska2Viktoriia Holubnycha3Yevhen Samokhin4Anna Yanovska5Julia Varava6Kateryna Diedkova7Łukasz Janus8Maksym Pogorielov9Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandBiomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, UkraineChitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both <i>E. coli</i> and <i>S. aureus</i> in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.https://www.mdpi.com/1420-3049/27/10/3343natural productschitosan electrospinningantibacterial biomaterials |
| spellingShingle | Viktoriia Korniienko Yevheniia Husak Julia Radwan-Pragłowska Viktoriia Holubnycha Yevhen Samokhin Anna Yanovska Julia Varava Kateryna Diedkova Łukasz Janus Maksym Pogorielov Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers Molecules natural products chitosan electrospinning antibacterial biomaterials |
| title | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
| title_full | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
| title_fullStr | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
| title_full_unstemmed | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
| title_short | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
| title_sort | impact of electrospinning parameters and post treatment method on antibacterial and antibiofilm activity of chitosan nanofibers |
| topic | natural products chitosan electrospinning antibacterial biomaterials |
| url | https://www.mdpi.com/1420-3049/27/10/3343 |
| work_keys_str_mv | AT viktoriiakorniienko impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT yevheniiahusak impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT juliaradwanpragłowska impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT viktoriiaholubnycha impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT yevhensamokhin impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT annayanovska impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT juliavarava impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT katerynadiedkova impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT łukaszjanus impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers AT maksympogorielov impactofelectrospinningparametersandposttreatmentmethodonantibacterialandantibiofilmactivityofchitosannanofibers |