Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i>
Z,Z-farnesol (Z,Z-FOH), the all-<i>cis</i> isomer of farnesol, holds enormous potential for application in cosmetics, daily chemicals, and pharmaceuticals. In this study, we aimed to metabolically engineer <i>Escherichia coli</i> to produce Z,Z-FOH. First, we tested five Z,Z-...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1583 |
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| author | Mengyang Lei Zetian Qiu Leilei Guan Zheng Xiang Guang-Rong Zhao |
| author_facet | Mengyang Lei Zetian Qiu Leilei Guan Zheng Xiang Guang-Rong Zhao |
| author_sort | Mengyang Lei |
| collection | DOAJ |
| description | Z,Z-farnesol (Z,Z-FOH), the all-<i>cis</i> isomer of farnesol, holds enormous potential for application in cosmetics, daily chemicals, and pharmaceuticals. In this study, we aimed to metabolically engineer <i>Escherichia coli</i> to produce Z,Z-FOH. First, we tested five Z,Z-farnesyl diphosphate (Z,Z-FPP) synthases that catalyze neryl diphosphate to form Z,Z-FPP in <i>E. coli</i>. Furthermore, we screened thirteen phosphatases that could facilitate the dephosphorylation of Z,Z-FPP to produce Z,Z-FOH. Finally, through site-directed mutagenesis of <i>cis</i>-prenyltransferase, the optimal mutant strain was able to produce 572.13 mg/L Z,Z-FOH by batch fermentation in a shake flask. This achievement represents the highest reported titer of Z,Z-FOH in microbes to date. Notably, this is the first report on the de novo biosynthesis of Z,Z-FOH in <i>E. coli</i>. This work represents a promising step toward developing synthetic <i>E. coli</i> cell factories for the de novo biosynthesis of Z,Z-FOH and other <i>cis</i>-configuration terpenoids. |
| first_indexed | 2024-03-11T02:08:14Z |
| format | Article |
| id | doaj.art-5f1eee264b0345f298cb2d27b6b56f67 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:14Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5f1eee264b0345f298cb2d27b6b56f672023-11-18T11:43:57ZengMDPI AGMicroorganisms2076-26072023-06-01116158310.3390/microorganisms11061583Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i>Mengyang Lei0Zetian Qiu1Leilei Guan2Zheng Xiang3Guang-Rong Zhao4Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, ChinaFrontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, ChinaFrontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, ChinaState Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, ChinaFrontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, ChinaZ,Z-farnesol (Z,Z-FOH), the all-<i>cis</i> isomer of farnesol, holds enormous potential for application in cosmetics, daily chemicals, and pharmaceuticals. In this study, we aimed to metabolically engineer <i>Escherichia coli</i> to produce Z,Z-FOH. First, we tested five Z,Z-farnesyl diphosphate (Z,Z-FPP) synthases that catalyze neryl diphosphate to form Z,Z-FPP in <i>E. coli</i>. Furthermore, we screened thirteen phosphatases that could facilitate the dephosphorylation of Z,Z-FPP to produce Z,Z-FOH. Finally, through site-directed mutagenesis of <i>cis</i>-prenyltransferase, the optimal mutant strain was able to produce 572.13 mg/L Z,Z-FOH by batch fermentation in a shake flask. This achievement represents the highest reported titer of Z,Z-FOH in microbes to date. Notably, this is the first report on the de novo biosynthesis of Z,Z-FOH in <i>E. coli</i>. This work represents a promising step toward developing synthetic <i>E. coli</i> cell factories for the de novo biosynthesis of Z,Z-FOH and other <i>cis</i>-configuration terpenoids.https://www.mdpi.com/2076-2607/11/6/1583(2Z,6Z)-farnesolenzyme engineeringmetabolic engineering<i>Escherichia coli</i>synthetic biology |
| spellingShingle | Mengyang Lei Zetian Qiu Leilei Guan Zheng Xiang Guang-Rong Zhao Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> Microorganisms (2Z,6Z)-farnesol enzyme engineering metabolic engineering <i>Escherichia coli</i> synthetic biology |
| title | Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> |
| title_full | Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> |
| title_fullStr | Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> |
| title_full_unstemmed | Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> |
| title_short | Metabolic Engineering for Efficient Production of Z,Z-Farnesol in <i>E. coli</i> |
| title_sort | metabolic engineering for efficient production of z z farnesol in i e coli i |
| topic | (2Z,6Z)-farnesol enzyme engineering metabolic engineering <i>Escherichia coli</i> synthetic biology |
| url | https://www.mdpi.com/2076-2607/11/6/1583 |
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