Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia
<p>Nucleophosmin (<i>NPM1</i>) gene mutations resulting in cytoplasmic delocalization of Nucleophosmin (NPMc+) are the most common genetic alteration in acute myeloid leukemia (AML). Here, we attempted to prepare monoclonal antibodies (mAbs) against <i>NPM1 </i>mutation...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2011-01-01
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| Series: | International Journal of Medical Sciences |
| Online Access: | http://www.medsci.org/v08p0309.htm |
| _version_ | 1818305392170500096 |
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| author | Shi Tan, Ling Zhang, Xiao-Ming Zhong, Zai-Lin Yang, Liu-Yang Zhao, Yu-Jie Gao, Hui-Yuan Shao, Feng-Xian Qin, Xian-Chun Chen, Hui-Juan Zhang, Hui Chen, Li Wang |
| author_facet | Shi Tan, Ling Zhang, Xiao-Ming Zhong, Zai-Lin Yang, Liu-Yang Zhao, Yu-Jie Gao, Hui-Yuan Shao, Feng-Xian Qin, Xian-Chun Chen, Hui-Juan Zhang, Hui Chen, Li Wang |
| author_sort | Shi Tan, Ling Zhang, Xiao-Ming Zhong, Zai-Lin Yang, Liu-Yang Zhao, Yu-Jie Gao, Hui-Yuan Shao, Feng-Xian Qin, Xian-Chun Chen, Hui-Juan Zhang, Hui Chen, Li Wang |
| collection | DOAJ |
| description | <p>Nucleophosmin (<i>NPM1</i>) gene mutations resulting in cytoplasmic delocalization of Nucleophosmin (NPMc+) are the most common genetic alteration in acute myeloid leukemia (AML). Here, we attempted to prepare monoclonal antibodies (mAbs) against <i>NPM1 </i>mutation A (NPM-mA) and investigated the mAbs' clinical utility in immunohistochemical detection of NPMc+AML. The pET-32a-NPM-mA vector with the whole open reading frame of the NPM-mA gene was constructed.<i> E.coli</i> BL21 transformed with the vector were induced to express the NPM-mA recombinant protein. BALB/c mice were immunized with the recombinant NPM-mA. Positive clones were selected by indirect ELISA and the mAbs were obtained. Immunohistochemistry was performed to detect the NPMc+ in bone marrow smears from 10 AML patients with NPM-mA. The results showed that the pET-32a-NPM-mA vector was successfully constructed and the NPM-mA recombinant protein was used to immunize the mice. Two positive clones (2G3 and 3F9) were selected. The mAbs against NPM-mA were raised, but did cross-react with wild type NPM1. The mAbs can be used to detect the cytoplasmic dislocation of NPM1 in all AMLs carrying NPM-mA. Our results show that anti-NPM-mA mAbs were produced. Though they would cross-react with wild type NPM1, the mAbs may still have potential in the detection of NPMc+AMLs.</p> |
| first_indexed | 2024-12-13T06:25:51Z |
| format | Article |
| id | doaj.art-5f23f077996a4709a9b6205efc33886c |
| institution | Directory Open Access Journal |
| issn | 1449-1907 |
| language | English |
| last_indexed | 2024-12-13T06:25:51Z |
| publishDate | 2011-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Medical Sciences |
| spelling | doaj.art-5f23f077996a4709a9b6205efc33886c2022-12-21T23:56:44ZengIvyspring International PublisherInternational Journal of Medical Sciences1449-19072011-01-0184309314Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid LeukemiaShi Tan, Ling Zhang, Xiao-Ming Zhong, Zai-Lin Yang, Liu-Yang Zhao, Yu-Jie Gao, Hui-Yuan Shao, Feng-Xian Qin, Xian-Chun Chen, Hui-Juan Zhang, Hui Chen, Li Wang<p>Nucleophosmin (<i>NPM1</i>) gene mutations resulting in cytoplasmic delocalization of Nucleophosmin (NPMc+) are the most common genetic alteration in acute myeloid leukemia (AML). Here, we attempted to prepare monoclonal antibodies (mAbs) against <i>NPM1 </i>mutation A (NPM-mA) and investigated the mAbs' clinical utility in immunohistochemical detection of NPMc+AML. The pET-32a-NPM-mA vector with the whole open reading frame of the NPM-mA gene was constructed.<i> E.coli</i> BL21 transformed with the vector were induced to express the NPM-mA recombinant protein. BALB/c mice were immunized with the recombinant NPM-mA. Positive clones were selected by indirect ELISA and the mAbs were obtained. Immunohistochemistry was performed to detect the NPMc+ in bone marrow smears from 10 AML patients with NPM-mA. The results showed that the pET-32a-NPM-mA vector was successfully constructed and the NPM-mA recombinant protein was used to immunize the mice. Two positive clones (2G3 and 3F9) were selected. The mAbs against NPM-mA were raised, but did cross-react with wild type NPM1. The mAbs can be used to detect the cytoplasmic dislocation of NPM1 in all AMLs carrying NPM-mA. Our results show that anti-NPM-mA mAbs were produced. Though they would cross-react with wild type NPM1, the mAbs may still have potential in the detection of NPMc+AMLs.</p>http://www.medsci.org/v08p0309.htm |
| spellingShingle | Shi Tan, Ling Zhang, Xiao-Ming Zhong, Zai-Lin Yang, Liu-Yang Zhao, Yu-Jie Gao, Hui-Yuan Shao, Feng-Xian Qin, Xian-Chun Chen, Hui-Juan Zhang, Hui Chen, Li Wang Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia International Journal of Medical Sciences |
| title | Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia |
| title_full | Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia |
| title_fullStr | Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia |
| title_full_unstemmed | Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia |
| title_short | Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia |
| title_sort | monoclonal antibodies against nucleophosmin mutants potentials for the detection of acute myeloid leukemia |
| url | http://www.medsci.org/v08p0309.htm |
| work_keys_str_mv | AT shitanlingzhangxiaomingzhongzailinyangliuyangzhaoyujiegaohuiyuanshaofengxianqinxianchunchenhuijuanzhanghuichenliwang monoclonalantibodiesagainstnucleophosminmutantspotentialsforthedetectionofacutemyeloidleukemia |