The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time
Objective(s): Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. Material and Methods: Adult female NMRI mice were divided in three...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_6643_ecf4e772ecee6f903c99377a9be94d64.pdf |
| _version_ | 1818394861324206080 |
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| author | Mandana Beigi Boroujeni Nasim Beigi Boroujeni Mohammadreza Gholami |
| author_facet | Mandana Beigi Boroujeni Nasim Beigi Boroujeni Mohammadreza Gholami |
| author_sort | Mandana Beigi Boroujeni |
| collection | DOAJ |
| description | Objective(s): Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. Material and Methods: Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimulation]. Uterine horn samples removed at pre-implantation time in each group. Motic image Plus 2 software was used to assess the quantitative vascular parameters of endometrium. Gene expression was determined for vascular endothelial growth factor (VEGF), FMS-like tyrosine kinase (FLT) and Kinase insert domain protein receptor (FLK)genes using the real time PCR method. Data analysis was done with LinReg PCR and Rest-RG software. Results: Comparison between progestrone treated mice after ovarian stimulation with control group showed that increase in rate of VEGF gene expression [0.775] and decrease in rate of FLK [6.072] and FLT [1.711] gene expression. Analysis of the data on quantitative vascular parameters were indicated remarkable increase in quantitative vascular parameters of progestrone treated mice compare to control group. Conclusion: Biological effect of progestrone on the vascular changes after ovarian stimulation resulted in an increase in VEGF receptors experession, it seems that induced angiogenesis by progesterone could result in better condition for implantation. |
| first_indexed | 2024-12-14T06:07:56Z |
| format | Article |
| id | doaj.art-5f2746299c77436abac96831042edfb2 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-14T06:07:56Z |
| publishDate | 2016-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-5f2746299c77436abac96831042edfb22022-12-21T23:14:13ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-03-011932522576643The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation timeMandana Beigi Boroujeni0Nasim Beigi Boroujeni1Mohammadreza Gholami2Deptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, IranRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranDeptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, IranObjective(s): Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. Material and Methods: Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimulation]. Uterine horn samples removed at pre-implantation time in each group. Motic image Plus 2 software was used to assess the quantitative vascular parameters of endometrium. Gene expression was determined for vascular endothelial growth factor (VEGF), FMS-like tyrosine kinase (FLT) and Kinase insert domain protein receptor (FLK)genes using the real time PCR method. Data analysis was done with LinReg PCR and Rest-RG software. Results: Comparison between progestrone treated mice after ovarian stimulation with control group showed that increase in rate of VEGF gene expression [0.775] and decrease in rate of FLK [6.072] and FLT [1.711] gene expression. Analysis of the data on quantitative vascular parameters were indicated remarkable increase in quantitative vascular parameters of progestrone treated mice compare to control group. Conclusion: Biological effect of progestrone on the vascular changes after ovarian stimulation resulted in an increase in VEGF receptors experession, it seems that induced angiogenesis by progesterone could result in better condition for implantation.http://ijbms.mums.ac.ir/article_6643_ecf4e772ecee6f903c99377a9be94d64.pdfAngiogenesisEndometriumGene expressionProgesteronePre-implantation timeVascular parameter |
| spellingShingle | Mandana Beigi Boroujeni Nasim Beigi Boroujeni Mohammadreza Gholami The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time Iranian Journal of Basic Medical Sciences Angiogenesis Endometrium Gene expression Progesterone Pre-implantation time Vascular parameter |
| title | The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time |
| title_full | The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time |
| title_fullStr | The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time |
| title_full_unstemmed | The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time |
| title_short | The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implatation time |
| title_sort | effect of progesterone treatment after ovarian induction on endometrial vegf gene expression and its receptors in mice at pre implatation time |
| topic | Angiogenesis Endometrium Gene expression Progesterone Pre-implantation time Vascular parameter |
| url | http://ijbms.mums.ac.ir/article_6643_ecf4e772ecee6f903c99377a9be94d64.pdf |
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