miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression
Intramuscular fat (IMF) is one of the crucial factors determining meat quality. IMF deposition depends on the hyperplasia and hypertrophy of intramuscular preadipocytes, in which genes and noncoding RNAs play an important regulatory role. According to previous transcriptome analysis, <i>ANXA6&...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/4/635 |
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| author | Zhongzhen Lin Yuan Tang Zhiqiang Li Jingjing Li Chunlin Yu Chaowu Yang Li Liu Yan Wang Yiping Liu |
| author_facet | Zhongzhen Lin Yuan Tang Zhiqiang Li Jingjing Li Chunlin Yu Chaowu Yang Li Liu Yan Wang Yiping Liu |
| author_sort | Zhongzhen Lin |
| collection | DOAJ |
| description | Intramuscular fat (IMF) is one of the crucial factors determining meat quality. IMF deposition depends on the hyperplasia and hypertrophy of intramuscular preadipocytes, in which genes and noncoding RNAs play an important regulatory role. According to previous transcriptome analysis, <i>ANXA6</i> and miR-24-3p were identified as involved in lipid metabolism in breast muscle. In this study, we further investigated their function in the proliferation and differentiation of chicken intramuscular preadipocytes. The results indicated that overexpression of <i>ANXA6</i> inhibited proliferation and promoted differentiation of intramuscular preadipocytes, while knockdown of <i>ANXA6</i> promoted cell proliferation and inhibited adipogenic differentiation. miR-24-3p was proved to directly bind to the 3′ untranslated region (3′UTR) of <i>ANXA6</i> by dual-luciferase reporter assay. The regulatory effect of miR-24-3p on the proliferation and differentiation of intramuscular preadipocytes was opposite to that of <i>ANXA6</i>. Besides, the overexpression vector of <i>ANXA6</i> eliminated the impact of miR-24-3p mimics on intramuscular preadipocytes. In brief, we revealed that miR-24-3p promoted proliferation but inhibited differentiation of intramuscular preadipocytes by blocking <i>ANXA6</i> expression, thus dominating IMF deposition in broilers. These findings may provide a novel target for improving chicken meat quality. |
| first_indexed | 2024-03-09T10:36:10Z |
| format | Article |
| id | doaj.art-5f27ff3ca1444bc3beb928549d96ec98 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:36:10Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-5f27ff3ca1444bc3beb928549d96ec982023-12-01T20:57:07ZengMDPI AGGenes2073-44252022-04-0113463510.3390/genes13040635miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> ExpressionZhongzhen Lin0Yuan Tang1Zhiqiang Li2Jingjing Li3Chunlin Yu4Chaowu Yang5Li Liu6Yan Wang7Yiping Liu8Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, ChinaAnimal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaIntramuscular fat (IMF) is one of the crucial factors determining meat quality. IMF deposition depends on the hyperplasia and hypertrophy of intramuscular preadipocytes, in which genes and noncoding RNAs play an important regulatory role. According to previous transcriptome analysis, <i>ANXA6</i> and miR-24-3p were identified as involved in lipid metabolism in breast muscle. In this study, we further investigated their function in the proliferation and differentiation of chicken intramuscular preadipocytes. The results indicated that overexpression of <i>ANXA6</i> inhibited proliferation and promoted differentiation of intramuscular preadipocytes, while knockdown of <i>ANXA6</i> promoted cell proliferation and inhibited adipogenic differentiation. miR-24-3p was proved to directly bind to the 3′ untranslated region (3′UTR) of <i>ANXA6</i> by dual-luciferase reporter assay. The regulatory effect of miR-24-3p on the proliferation and differentiation of intramuscular preadipocytes was opposite to that of <i>ANXA6</i>. Besides, the overexpression vector of <i>ANXA6</i> eliminated the impact of miR-24-3p mimics on intramuscular preadipocytes. In brief, we revealed that miR-24-3p promoted proliferation but inhibited differentiation of intramuscular preadipocytes by blocking <i>ANXA6</i> expression, thus dominating IMF deposition in broilers. These findings may provide a novel target for improving chicken meat quality.https://www.mdpi.com/2073-4425/13/4/635miRNAs<i>ANXA6</i>intramuscular fatlipogenesisavian |
| spellingShingle | Zhongzhen Lin Yuan Tang Zhiqiang Li Jingjing Li Chunlin Yu Chaowu Yang Li Liu Yan Wang Yiping Liu miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression Genes miRNAs <i>ANXA6</i> intramuscular fat lipogenesis avian |
| title | miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression |
| title_full | miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression |
| title_fullStr | miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression |
| title_full_unstemmed | miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression |
| title_short | miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking <i>ANXA6</i> Expression |
| title_sort | mir 24 3p dominates the proliferation and differentiation of chicken intramuscular preadipocytes by blocking i anxa6 i expression |
| topic | miRNAs <i>ANXA6</i> intramuscular fat lipogenesis avian |
| url | https://www.mdpi.com/2073-4425/13/4/635 |
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