Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for th...
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.677842/full |
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| author | Anusha Rai Uppada Jagadeeshwari Gupta Deepshikha Nandardhane Smita Chintalapati Sasikala Chintalapati Venkata Ramana |
| author_facet | Anusha Rai Uppada Jagadeeshwari Gupta Deepshikha Nandardhane Smita Chintalapati Sasikala Chintalapati Venkata Ramana |
| author_sort | Anusha Rai |
| collection | DOAJ |
| description | The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Roseomonas. Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing Roseomonas species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (dDDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus Roseomonas and other interspersing members of the family Acetobacteraceae were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus Roseomonas and propose six novel genera as Pararoseomonas gen. nov., Falsiroseomonas gen. nov., Paeniroseomonas gen. nov., Plastoroseomonas gen. nov., Neoroseomonas gen. nov., and Pseudoroseomonas gen. nov. |
| first_indexed | 2024-12-16T18:49:56Z |
| format | Article |
| id | doaj.art-5f29e8ec012a4b8aa4923c159a8d9156 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T18:49:56Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5f29e8ec012a4b8aa4923c159a8d91562022-12-21T22:20:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.677842677842Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New GeneraAnusha Rai0Uppada Jagadeeshwari1Gupta Deepshikha2Nandardhane Smita3Chintalapati Sasikala4Chintalapati Venkata Ramana5Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaBacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology (IST), Jawaharlal Nehru Technological (JNT) University Hyderabad, Hyderabad, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaBacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology (IST), Jawaharlal Nehru Technological (JNT) University Hyderabad, Hyderabad, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaThe genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Roseomonas. Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing Roseomonas species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (dDDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus Roseomonas and other interspersing members of the family Acetobacteraceae were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus Roseomonas and propose six novel genera as Pararoseomonas gen. nov., Falsiroseomonas gen. nov., Paeniroseomonas gen. nov., Plastoroseomonas gen. nov., Neoroseomonas gen. nov., and Pseudoroseomonas gen. nov.https://www.frontiersin.org/articles/10.3389/fmicb.2021.677842/fullphylotaxogenomicsaverage amino acid Identity (AAI)percentage of conserved proteins (POCP)reclassificationRoseomonas |
| spellingShingle | Anusha Rai Uppada Jagadeeshwari Gupta Deepshikha Nandardhane Smita Chintalapati Sasikala Chintalapati Venkata Ramana Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera Frontiers in Microbiology phylotaxogenomics average amino acid Identity (AAI) percentage of conserved proteins (POCP) reclassification Roseomonas |
| title | Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera |
| title_full | Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera |
| title_fullStr | Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera |
| title_full_unstemmed | Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera |
| title_short | Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera |
| title_sort | phylotaxogenomics for the reappraisal of the genus roseomonas with the creation of six new genera |
| topic | phylotaxogenomics average amino acid Identity (AAI) percentage of conserved proteins (POCP) reclassification Roseomonas |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.677842/full |
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