Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals
We report the presence of the <i>mcr-1</i> gene among 880 <i>Escherichia coli</i> clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/488 |
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| author | Elsa De La Cadena Mateo Mahecha Ana María Velandia Juan Carlos García-Betancur Laura J. Rojas Jessica Porras Christian Pallares María Virginia Villegas |
| author_facet | Elsa De La Cadena Mateo Mahecha Ana María Velandia Juan Carlos García-Betancur Laura J. Rojas Jessica Porras Christian Pallares María Virginia Villegas |
| author_sort | Elsa De La Cadena |
| collection | DOAJ |
| description | We report the presence of the <i>mcr-1</i> gene among 880 <i>Escherichia coli</i> clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were screened for the presence of the <i>mcr-1</i> gene; five carried the gene. These five isolates were subjected to whole genome sequencing (WGS) to identify additional resistomes and their ST. In addition, antimicrobial susceptibility testing revealed that all <i>E. coli</i> isolates carrying <i>mcr-1</i> were susceptible to third generation-cephalosporin and carbapenems, except one, which carried an extended-spectrum β-lactamase (CTX-M-55), along with the fosfomycin resistance encoding gene, <i>fosA</i>. WGS indicated that these isolates belonged to four distinct sequence types (ST58, ST46, ST393, and a newly described ST14315) and to phylogroups B1, A, and D. In this geographic region, the spread of <i>mcr-1</i> in <i>E. coli</i> is low and has not been inserted into high-risk clones such as ST131, which has been present in the country longer. |
| first_indexed | 2024-03-11T07:01:26Z |
| format | Article |
| id | doaj.art-5f2a9db4c47a440bbc4e191781b17981 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T07:01:26Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5f2a9db4c47a440bbc4e191781b179812023-11-17T09:13:42ZengMDPI AGAntibiotics2079-63822023-03-0112348810.3390/antibiotics12030488Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian HospitalsElsa De La Cadena0Mateo Mahecha1Ana María Velandia2Juan Carlos García-Betancur3Laura J. Rojas4Jessica Porras5Christian Pallares6María Virginia Villegas7Grupo de Investigación en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogotá 110121, ColombiaGrupo de Investigación REMA, Facultad de Ciencias de la Salud, Programa de Bacteriología y Laboratorio Clínico, Universidad Colegio Mayor de Cundinamarca, Bogotá 110311, ColombiaGrupo de Investigación REMA, Facultad de Ciencias de la Salud, Programa de Bacteriología y Laboratorio Clínico, Universidad Colegio Mayor de Cundinamarca, Bogotá 110311, ColombiaGrupo de Investigación en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogotá 110121, ColombiaDepartment of Microbiology and Molecular Biology, Case Western Reserve University, Cleveland, OH 44106-7164, USAGrupo de Investigación en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogotá 110121, ColombiaGrupo de Investigación en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogotá 110121, ColombiaGrupo de Investigación en Resistencia Antimicrobiana y Epidemiologia Hospitalaria, Universidad El Bosque, Bogotá 110121, ColombiaWe report the presence of the <i>mcr-1</i> gene among 880 <i>Escherichia coli</i> clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) isolates were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant isolates were screened for the presence of the <i>mcr-1</i> gene; five carried the gene. These five isolates were subjected to whole genome sequencing (WGS) to identify additional resistomes and their ST. In addition, antimicrobial susceptibility testing revealed that all <i>E. coli</i> isolates carrying <i>mcr-1</i> were susceptible to third generation-cephalosporin and carbapenems, except one, which carried an extended-spectrum β-lactamase (CTX-M-55), along with the fosfomycin resistance encoding gene, <i>fosA</i>. WGS indicated that these isolates belonged to four distinct sequence types (ST58, ST46, ST393, and a newly described ST14315) and to phylogroups B1, A, and D. In this geographic region, the spread of <i>mcr-1</i> in <i>E. coli</i> is low and has not been inserted into high-risk clones such as ST131, which has been present in the country longer.https://www.mdpi.com/2079-6382/12/3/488<i>Escherichia coli</i>colistin resistance<i>mcr-1</i> |
| spellingShingle | Elsa De La Cadena Mateo Mahecha Ana María Velandia Juan Carlos García-Betancur Laura J. Rojas Jessica Porras Christian Pallares María Virginia Villegas Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals Antibiotics <i>Escherichia coli</i> colistin resistance <i>mcr-1</i> |
| title | Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals |
| title_full | Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals |
| title_fullStr | Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals |
| title_full_unstemmed | Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals |
| title_short | Identification of <i>mcr-1</i> Genes and Characterization of Resistance Mechanisms to Colistin in <i>Escherichia coli</i> Isolates from Colombian Hospitals |
| title_sort | identification of i mcr 1 i genes and characterization of resistance mechanisms to colistin in i escherichia coli i isolates from colombian hospitals |
| topic | <i>Escherichia coli</i> colistin resistance <i>mcr-1</i> |
| url | https://www.mdpi.com/2079-6382/12/3/488 |
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