Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone
The objective of the present study was to investigate the effects of chronic lead exposure on the mineral properties of alveolar bone. For this purpose, female Wistar rats (<i>n</i> = 8) were exposed to 1000 ppm lead acetate in drinking water for 90 days, while the control group (<i&g...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Minerals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-163X/11/6/642 |
| _version_ | 1797529898787536896 |
|---|---|
| author | Pedro Álvarez-Lloret Cristina Benavides-Reyes Ching Ming Lee María Pilar Martínez María Inés Conti Alejandro B. Rodríguez-Navarro Santiago González-López Alberto Perez-Huerta Antonela Romina Terrizzi |
| author_facet | Pedro Álvarez-Lloret Cristina Benavides-Reyes Ching Ming Lee María Pilar Martínez María Inés Conti Alejandro B. Rodríguez-Navarro Santiago González-López Alberto Perez-Huerta Antonela Romina Terrizzi |
| author_sort | Pedro Álvarez-Lloret |
| collection | DOAJ |
| description | The objective of the present study was to investigate the effects of chronic lead exposure on the mineral properties of alveolar bone. For this purpose, female Wistar rats (<i>n</i> = 8) were exposed to 1000 ppm lead acetate in drinking water for 90 days, while the control group (<i>n</i> = 5) was treated with sodium acetate. The alveolar bone structure and chemical composition of the dissected mandibles were examined using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), inductively coupled plasma optical emission spectrometry (ICP-OES), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD) techniques to determine possible alterations in alveolar bone due to lead exposure. In addition, changes in bone mechanical properties were analysed using a three-point bending test. Exposure to lead induced notable changes in bone mineralization and properties, specifically a reduction of the trabecular thickness and bone mineral density. Furthermore, there was a reduction in carbonate content and an increase in bone mineral crystallinity. These changes in bone mineralization could be explained by an alteration in bone turnover due to lead exposure. Three-point bending showed a trend of decreased displacement at failure in the mandibles of lead-exposed rats, which could compromise the mechanical stability and normal development of the dentition. |
| first_indexed | 2024-03-10T10:20:24Z |
| format | Article |
| id | doaj.art-5f2b89fa54ee4900be37245b0d6fb11f |
| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-10T10:20:24Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Minerals |
| spelling | doaj.art-5f2b89fa54ee4900be37245b0d6fb11f2023-11-22T00:26:19ZengMDPI AGMinerals2075-163X2021-06-0111664210.3390/min11060642Chronic Lead Exposure Alters Mineral Properties in Alveolar BonePedro Álvarez-Lloret0Cristina Benavides-Reyes1Ching Ming Lee2María Pilar Martínez3María Inés Conti4Alejandro B. Rodríguez-Navarro5Santiago González-López6Alberto Perez-Huerta7Antonela Romina Terrizzi8Department of Geology, Faculty of Geology, University of Oviedo, 33005 Oviedo, SpainDepartment of Operative Dentistry, School of Dentistry, University of Granada, 18071 Granada, SpainDepartment of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires C1122, ArgentinaDepartment of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires C1122, ArgentinaDepartment of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires C1122, ArgentinaDepartment of Mineralogy and Petrology, Faculty of Sciences, University of Granada, 18002 Granada, SpainDepartment of Operative Dentistry, School of Dentistry, University of Granada, 18071 Granada, SpainDepartment of Geological Sciences, University of Alabama, Tuscaloosa, AL 35487, USADepartment of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires C1122, ArgentinaThe objective of the present study was to investigate the effects of chronic lead exposure on the mineral properties of alveolar bone. For this purpose, female Wistar rats (<i>n</i> = 8) were exposed to 1000 ppm lead acetate in drinking water for 90 days, while the control group (<i>n</i> = 5) was treated with sodium acetate. The alveolar bone structure and chemical composition of the dissected mandibles were examined using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), inductively coupled plasma optical emission spectrometry (ICP-OES), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD) techniques to determine possible alterations in alveolar bone due to lead exposure. In addition, changes in bone mechanical properties were analysed using a three-point bending test. Exposure to lead induced notable changes in bone mineralization and properties, specifically a reduction of the trabecular thickness and bone mineral density. Furthermore, there was a reduction in carbonate content and an increase in bone mineral crystallinity. These changes in bone mineralization could be explained by an alteration in bone turnover due to lead exposure. Three-point bending showed a trend of decreased displacement at failure in the mandibles of lead-exposed rats, which could compromise the mechanical stability and normal development of the dentition.https://www.mdpi.com/2075-163X/11/6/642lead intoxicationalveolar processbone mineralizationtrabecular morphologyapatite crystallinitymechanical properties |
| spellingShingle | Pedro Álvarez-Lloret Cristina Benavides-Reyes Ching Ming Lee María Pilar Martínez María Inés Conti Alejandro B. Rodríguez-Navarro Santiago González-López Alberto Perez-Huerta Antonela Romina Terrizzi Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone Minerals lead intoxication alveolar process bone mineralization trabecular morphology apatite crystallinity mechanical properties |
| title | Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone |
| title_full | Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone |
| title_fullStr | Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone |
| title_full_unstemmed | Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone |
| title_short | Chronic Lead Exposure Alters Mineral Properties in Alveolar Bone |
| title_sort | chronic lead exposure alters mineral properties in alveolar bone |
| topic | lead intoxication alveolar process bone mineralization trabecular morphology apatite crystallinity mechanical properties |
| url | https://www.mdpi.com/2075-163X/11/6/642 |
| work_keys_str_mv | AT pedroalvarezlloret chronicleadexposurealtersmineralpropertiesinalveolarbone AT cristinabenavidesreyes chronicleadexposurealtersmineralpropertiesinalveolarbone AT chingminglee chronicleadexposurealtersmineralpropertiesinalveolarbone AT mariapilarmartinez chronicleadexposurealtersmineralpropertiesinalveolarbone AT mariainesconti chronicleadexposurealtersmineralpropertiesinalveolarbone AT alejandrobrodrigueznavarro chronicleadexposurealtersmineralpropertiesinalveolarbone AT santiagogonzalezlopez chronicleadexposurealtersmineralpropertiesinalveolarbone AT albertoperezhuerta chronicleadexposurealtersmineralpropertiesinalveolarbone AT antonelarominaterrizzi chronicleadexposurealtersmineralpropertiesinalveolarbone |