Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis

Ovarian cancer is the second leading cause of death in women with gynecological malignancy in China. Circular RNAs are a class of noncoding regulatory RNAs reported to be involved in cancer development and progression. Previous studies, including our own, have indicated that hsa_circ_0007444 is down...

Full description

Bibliographic Details
Main Authors: Zhang Min, Sun Yu, Xu Hanzi, Shi Yaqian, Shen Rong, Teng Fang, Xu Juan, Jia Xuemei
Format: Article
Language:English
Published: China Science Publishing & Media Ltd. 2023-04-01
Series:Acta Biochimica et Biophysica Sinica
Subjects:
Online Access:https://www.sciengine.com/doi/10.3724/abbs.2023052
_version_ 1797635811494068224
author Zhang Min
Sun Yu
Xu Hanzi
Shi Yaqian
Shen Rong
Teng Fang
Xu Juan
Jia Xuemei
author_facet Zhang Min
Sun Yu
Xu Hanzi
Shi Yaqian
Shen Rong
Teng Fang
Xu Juan
Jia Xuemei
author_sort Zhang Min
collection DOAJ
description Ovarian cancer is the second leading cause of death in women with gynecological malignancy in China. Circular RNAs are a class of noncoding regulatory RNAs reported to be involved in cancer development and progression. Previous studies, including our own, have indicated that hsa_circ_0007444 is downregulated in ovarian cancer tissues. This study aims to elucidate the function and mechanism of hsa_circ_0007444 in ovarian cancer progression. The expression of hsa_circ_0007444 is determined by quantitative real-time PCR (qRT-PCR). Cell proliferation, invasion, migration and apoptosis are examined by cell counting-kit 8 (CCK-8), transwell and flow cytometry assays. Tumor growth and metastasis are assessed in vivo using Balb/c nude mouse xenograft model and tail vein injection model. And the mechanism of action of hsa_circ_0007444 is analysed by RNA-binding protein immunoprecipitation (RIP), luciferase reporter and rescue assays. hsa_circ_0007444 is downregulated in ovarian cancer tissues and cell lines compared with that in normal ovarian tissues and normal epithelial cell line. Gain- and loss-of-function results indicate that hsa_circ_0007444 inhibits cell proliferation, invasion, migration and increases cell apoptosis of ovarian cancer cells in vitro, and inhibits tumor growth and lung metastasis in vivo. Mechanistically, hsa_circ_0007444 can interact with AGO2 and sponge miR-23a-3p, thereby upregulating DICER1 expression, which is an important tumor suppressor in ovarian cancer. And miR-23a-3p mimics can rescue the inhibitory effect of hsa_circ_0007444 on ovarian cancer cell proliferation, invasion and migration. Therefore, hsa_circ_0007444 can inhibit ovarian cancer progression through the hsa_circ_0007444/miR-23a-3p/DICER1 axis.
first_indexed 2024-03-11T12:26:40Z
format Article
id doaj.art-5f2d301019464e4a95adf219f934330a
institution Directory Open Access Journal
issn 1672-9145
language English
last_indexed 2024-03-11T12:26:40Z
publishDate 2023-04-01
publisher China Science Publishing & Media Ltd.
record_format Article
series Acta Biochimica et Biophysica Sinica
spelling doaj.art-5f2d301019464e4a95adf219f934330a2023-11-06T08:49:34ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-04-015557458610.3724/abbs.202305220d259ccCircular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axisZhang Min0Sun Yu1Xu Hanzi2Shi Yaqian3Shen Rong4Teng Fang5Xu Juan6Jia Xuemei7["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China","Department of Gynecology, Gannan Medical University, Ganzhou 341000, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]["Jiangsu Institute of Cancer Research, the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]["Department of Gynecology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China"]Ovarian cancer is the second leading cause of death in women with gynecological malignancy in China. Circular RNAs are a class of noncoding regulatory RNAs reported to be involved in cancer development and progression. Previous studies, including our own, have indicated that hsa_circ_0007444 is downregulated in ovarian cancer tissues. This study aims to elucidate the function and mechanism of hsa_circ_0007444 in ovarian cancer progression. The expression of hsa_circ_0007444 is determined by quantitative real-time PCR (qRT-PCR). Cell proliferation, invasion, migration and apoptosis are examined by cell counting-kit 8 (CCK-8), transwell and flow cytometry assays. Tumor growth and metastasis are assessed in vivo using Balb/c nude mouse xenograft model and tail vein injection model. And the mechanism of action of hsa_circ_0007444 is analysed by RNA-binding protein immunoprecipitation (RIP), luciferase reporter and rescue assays. hsa_circ_0007444 is downregulated in ovarian cancer tissues and cell lines compared with that in normal ovarian tissues and normal epithelial cell line. Gain- and loss-of-function results indicate that hsa_circ_0007444 inhibits cell proliferation, invasion, migration and increases cell apoptosis of ovarian cancer cells in vitro, and inhibits tumor growth and lung metastasis in vivo. Mechanistically, hsa_circ_0007444 can interact with AGO2 and sponge miR-23a-3p, thereby upregulating DICER1 expression, which is an important tumor suppressor in ovarian cancer. And miR-23a-3p mimics can rescue the inhibitory effect of hsa_circ_0007444 on ovarian cancer cell proliferation, invasion and migration. Therefore, hsa_circ_0007444 can inhibit ovarian cancer progression through the hsa_circ_0007444/miR-23a-3p/DICER1 axis. https://www.sciengine.com/doi/10.3724/abbs.2023052ovarian cancerhsa_circ_0007444miR-23a-3pDICER1progression
spellingShingle Zhang Min
Sun Yu
Xu Hanzi
Shi Yaqian
Shen Rong
Teng Fang
Xu Juan
Jia Xuemei
Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
Acta Biochimica et Biophysica Sinica
ovarian cancer
hsa_circ_0007444
miR-23a-3p
DICER1
progression
title Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
title_full Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
title_fullStr Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
title_full_unstemmed Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
title_short Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis
title_sort circular rna hsa circ 0007444 inhibits ovarian cancer progression through mir 23a 3p dicer1 axis
topic ovarian cancer
hsa_circ_0007444
miR-23a-3p
DICER1
progression
url https://www.sciengine.com/doi/10.3724/abbs.2023052
work_keys_str_mv AT zhangmin circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT sunyu circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT xuhanzi circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT shiyaqian circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT shenrong circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT tengfang circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT xujuan circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis
AT jiaxuemei circularrnahsacirc0007444inhibitsovariancancerprogressionthroughmir23a3pdicer1axis