CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli

CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli

Abstract Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We used Cas9‐mediated recombineering to introduce a libra...

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Bibliographic Details
Main Authors: Alaksh Choudhury, Jacob A Fenster, Reilly G Fankhauser, Joel L Kaar, Olivier Tenaillon, Ryan T Gill
Format: Article
Language:English
Published: Springer Nature 2020-03-01
Series:Molecular Systems Biology
Subjects:
CRISPR‐Cas9
deep mutational scanning
essential genes
genome editing
recombineering
Online Access:https://doi.org/10.15252/msb.20199265

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