Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications
Superparamagnetic nanoparticles have seen increased potential in medical and environmental applications. Their preparation is traditionally made by the coprecipitation method, with limited control over the particle size distribution. Microemulsion methods could be advantageous due to the efficient c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/1/427 |
| _version_ | 1797542392166875136 |
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| author | María Salvador Gemma Gutiérrez Sara Noriega Amanda Moyano María Carmen Blanco-López María Matos |
| author_facet | María Salvador Gemma Gutiérrez Sara Noriega Amanda Moyano María Carmen Blanco-López María Matos |
| author_sort | María Salvador |
| collection | DOAJ |
| description | Superparamagnetic nanoparticles have seen increased potential in medical and environmental applications. Their preparation is traditionally made by the coprecipitation method, with limited control over the particle size distribution. Microemulsion methods could be advantageous due to the efficient control of the size, shape, and composition of the nanoparticles obtained. Water-in-oil (W/O) microemulsions consist of aqueous microdomains dispersed in a continuous oil phase, stabilized by surfactant molecules. These work as nanoreactors where the synthesis of the desired nanoparticles takes place through a co-precipitation chemical reaction. In this work, superparamagnetic magnetite nanoparticles with average diameters between 5.4 and 7.2 nm and large monodispersity have been synthesized through precipitation in a W/O microemulsion, with Cetyl Trimethyl Ammonium Bromide (CTAB) as a main surfactant, 1-butanol as a cosurfactant, and with 1-hexanol as the continuous oily phase. The optimization of the corresponding washing protocol has also been established since a strict control is required when using these materials for bioapplications. Their applicability in those has been proved by their encapsulation in liposomes, being tested as signal enhancers for lateral flow immunoassays by using the affinity neutravidin-biotin model system. Due to their magnetic behaviour, they were also tested for magnetic separation. These novel materials have been found to be useful for analytical applications requiring high sensitivity and the removal of interferences. |
| first_indexed | 2024-03-10T13:29:56Z |
| format | Article |
| id | doaj.art-5f2e4270c4d341f6b8cd867b254b4985 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:29:56Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f2e4270c4d341f6b8cd867b254b49852023-11-21T08:05:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122142710.3390/ijms22010427Microemulsion Synthesis of Superparamagnetic Nanoparticles for BioapplicationsMaría Salvador0Gemma Gutiérrez1Sara Noriega2Amanda Moyano3María Carmen Blanco-López4María Matos5Department of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, Julián Clavería 8, 33006 Oviedo, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, Julián Clavería 8, 33006 Oviedo, SpainInstituto Universitario de Biotecnología de Asturias, University of Oviedo, 33006 Oviedo, SpainInstituto Universitario de Biotecnología de Asturias, University of Oviedo, 33006 Oviedo, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, Julián Clavería 8, 33006 Oviedo, SpainSuperparamagnetic nanoparticles have seen increased potential in medical and environmental applications. Their preparation is traditionally made by the coprecipitation method, with limited control over the particle size distribution. Microemulsion methods could be advantageous due to the efficient control of the size, shape, and composition of the nanoparticles obtained. Water-in-oil (W/O) microemulsions consist of aqueous microdomains dispersed in a continuous oil phase, stabilized by surfactant molecules. These work as nanoreactors where the synthesis of the desired nanoparticles takes place through a co-precipitation chemical reaction. In this work, superparamagnetic magnetite nanoparticles with average diameters between 5.4 and 7.2 nm and large monodispersity have been synthesized through precipitation in a W/O microemulsion, with Cetyl Trimethyl Ammonium Bromide (CTAB) as a main surfactant, 1-butanol as a cosurfactant, and with 1-hexanol as the continuous oily phase. The optimization of the corresponding washing protocol has also been established since a strict control is required when using these materials for bioapplications. Their applicability in those has been proved by their encapsulation in liposomes, being tested as signal enhancers for lateral flow immunoassays by using the affinity neutravidin-biotin model system. Due to their magnetic behaviour, they were also tested for magnetic separation. These novel materials have been found to be useful for analytical applications requiring high sensitivity and the removal of interferences.https://www.mdpi.com/1422-0067/22/1/427superparamagnetic iron oxide nanoparticlesmicroemulsionco-precipitationliposomeslateral flow immunoassaypoint-of-care analytical platforms |
| spellingShingle | María Salvador Gemma Gutiérrez Sara Noriega Amanda Moyano María Carmen Blanco-López María Matos Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications International Journal of Molecular Sciences superparamagnetic iron oxide nanoparticles microemulsion co-precipitation liposomes lateral flow immunoassay point-of-care analytical platforms |
| title | Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications |
| title_full | Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications |
| title_fullStr | Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications |
| title_full_unstemmed | Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications |
| title_short | Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications |
| title_sort | microemulsion synthesis of superparamagnetic nanoparticles for bioapplications |
| topic | superparamagnetic iron oxide nanoparticles microemulsion co-precipitation liposomes lateral flow immunoassay point-of-care analytical platforms |
| url | https://www.mdpi.com/1422-0067/22/1/427 |
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