Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study
Background and Objective: Group A beta-hemolytic streptococcus (GABHS) is an important pharyngotonsillitis etiologic agent in children. The objective of this study was diagnosis of streptococcal pharyngitis based on rapid antigen detection test and conventional pharyngeal culture. Materials and Meth...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-06-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/95 |
| _version_ | 1818871238730186752 |
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| author | S Noorbakhsh A Tabatabaei M Farhadi F Ebrahimi Taj |
| author_facet | S Noorbakhsh A Tabatabaei M Farhadi F Ebrahimi Taj |
| author_sort | S Noorbakhsh |
| collection | DOAJ |
| description | Background and Objective: Group A beta-hemolytic streptococcus (GABHS) is an important pharyngotonsillitis etiologic agent in children. The objective of this study was diagnosis of streptococcal pharyngitis based on rapid antigen detection test and conventional pharyngeal culture.
Materials and Methods: The rapid GABHS antigen detection test was compared to culture on blood agar, the gold standard for the diagnosis of this etiologic agent.
Results: Streptococcal antigen was detected in pharyngeal specimens of 34.5% of cases by rapid strip test. We detected group A Streptococcus in 17.2% of pharyngeal culture. There was no agreement between two methods ( PV < 0.1). The negative pharyngeal culture results are probably due to antibiotic usage in 43.2 % of patients. Positive rapid test results in pharyngeal swab was age dependent ( P < 0.05). There was good correlation between observing the "petechia in pharynx of patients" and positive rapid test in pharyngeal swab (P < 0.004). Throat culture results were relatated to previous antibiotic usage ( P < 0.03).
Conclusion: The rapid test in pharyngeal swab is helpful for rapid diagnosis and treatment of GABHS pharyngitis. Diagnosis of GABHS pharyngitis based on soley clinical findings is misleading in the majority of cases. Petechia observed in pharynx of the cases was highly predictive of streptococcal pharyngitis. |
| first_indexed | 2024-12-19T12:19:45Z |
| format | Article |
| id | doaj.art-5f3104b9d9dd46219ed69357c6b9ac85 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T12:19:45Z |
| publishDate | 2011-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-5f3104b9d9dd46219ed69357c6b9ac852022-12-21T20:21:51ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472011-06-0132Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional studyS Noorbakhsh0A Tabatabaei1M Farhadi2F Ebrahimi Taj3Pediatric Infectious Diseases Research center,Tehran University of Medical Sciences, Tehran, Iran.Pediatric Infectious Diseases Research center,Tehran University of Medical Sciences, Tehran, Iran.Research Center for Diseases of Ear, Nose and Throat, Tehran University of Medical Sciences, Tehran,Iran.Pediatric Infectious Diseases Research center,Tehran University of Medical Sciences, Tehran, Iran.Background and Objective: Group A beta-hemolytic streptococcus (GABHS) is an important pharyngotonsillitis etiologic agent in children. The objective of this study was diagnosis of streptococcal pharyngitis based on rapid antigen detection test and conventional pharyngeal culture. Materials and Methods: The rapid GABHS antigen detection test was compared to culture on blood agar, the gold standard for the diagnosis of this etiologic agent. Results: Streptococcal antigen was detected in pharyngeal specimens of 34.5% of cases by rapid strip test. We detected group A Streptococcus in 17.2% of pharyngeal culture. There was no agreement between two methods ( PV < 0.1). The negative pharyngeal culture results are probably due to antibiotic usage in 43.2 % of patients. Positive rapid test results in pharyngeal swab was age dependent ( P < 0.05). There was good correlation between observing the "petechia in pharynx of patients" and positive rapid test in pharyngeal swab (P < 0.004). Throat culture results were relatated to previous antibiotic usage ( P < 0.03). Conclusion: The rapid test in pharyngeal swab is helpful for rapid diagnosis and treatment of GABHS pharyngitis. Diagnosis of GABHS pharyngitis based on soley clinical findings is misleading in the majority of cases. Petechia observed in pharynx of the cases was highly predictive of streptococcal pharyngitis.https://ijm.tums.ac.ir/index.php/ijm/article/view/95PharyngitisGroup A beta hemolytic Streptococcus (GABHS)Immunochromatograhic rapid test |
| spellingShingle | S Noorbakhsh A Tabatabaei M Farhadi F Ebrahimi Taj Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study Iranian Journal of Microbiology Pharyngitis Group A beta hemolytic Streptococcus (GABHS) Immunochromatograhic rapid test |
| title | Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study |
| title_full | Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study |
| title_fullStr | Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study |
| title_full_unstemmed | Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study |
| title_short | Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus pharyngitis in children: A cross/ sectional study |
| title_sort | immunoasssay chromatographic antigen test for rapid diagnosis of group a beta hemolytic streptococcus pharyngitis in children a cross sectional study |
| topic | Pharyngitis Group A beta hemolytic Streptococcus (GABHS) Immunochromatograhic rapid test |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/95 |
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