Effect of letrozole and raloxifene on in vitro human endometrial stromal cells
Introduction: Letrozole and raloxifene are used in female reproductive disease. They have special properties and side-effects. This study was performed with aim to compare the in vitro effects of letrozole and raloxifene on human endometrial stromal cells. Methods: This experimental study was perfor...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Mashhad University of Medical Sciences
2020-07-01
|
| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| Subjects: | |
| Online Access: | https://ijogi.mums.ac.ir/article_16611_44f6512b6e359aee28120348d0bc076f.pdf |
| _version_ | 1811328771324641280 |
|---|---|
| author | Maryam Pourheydar Zahra Rashidi Mozafar Khazaei |
| author_facet | Maryam Pourheydar Zahra Rashidi Mozafar Khazaei |
| author_sort | Maryam Pourheydar |
| collection | DOAJ |
| description | Introduction: Letrozole and raloxifene are used in female reproductive disease. They have special properties and side-effects. This study was performed with aim to compare the in vitro effects of letrozole and raloxifene on human endometrial stromal cells.
Methods: This experimental study was performed in 2019 on 16 samples of endometrial biopsy of women with normal menstrual cycles in secretory phase. Endometrial biopsies were digested by collagenase type I. Stromal cells were harvested by using serial filtration of cell suspension through cell strainer (70 and 40 µm) and ficoll layering. The cells were confirmed by immunohistochemical staining for vimentin. The cells of each biopsy were divided into: control and letrozole, or control and raloxifene. After 15 day, morphological changes and cell viability were determined. Data were analyzed by one way ANOVA and Tukey test.
Results: Cell viability in control and letrozole 0.1, 1 and 10 µM concentration was 14.7, 7.8, 5.6 and 4.2 ×104 cells, respectively. Cell viability in control and raloxifene 0.1, 1 and 10 µM was 21.8, 12.9, 9.1 and 3×104 cells, respectively; the difference was significant (P<0.05). The morphological changes score of cells were 3.86, 3.38, 2.68 and 2.08 in control and letrozole groups, and were 3.98, 3.77, 3.72, 1.18 in control and raloxifene; the difference was significant (P<0.05). The effect of letrozole and raloxifene on the bioavailability and morphology of endometrial stromal cells was different.
Conclusion: Letrozole and raloxifene showed anti-proliferative and dose-dependent effects on in vitro human endometrial stromal cells and raloxifene effect was more potent than letrozole. |
| first_indexed | 2024-04-13T15:32:08Z |
| format | Article |
| id | doaj.art-5f313d2b42c74097b89cc0770eab83d6 |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-04-13T15:32:08Z |
| publishDate | 2020-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-5f313d2b42c74097b89cc0770eab83d62022-12-22T02:41:22ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632020-07-01235263410.22038/ijogi.2020.1661116611Effect of letrozole and raloxifene on in vitro human endometrial stromal cellsMaryam Pourheydar0Zahra Rashidi1Mozafar Khazaei2PhD of Histology, Department of Anatomical Sciences, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.Assistant Professor, PhD of Reproductive Biology, Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Professor, PhD of Anatomical Sciences, Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Introduction: Letrozole and raloxifene are used in female reproductive disease. They have special properties and side-effects. This study was performed with aim to compare the in vitro effects of letrozole and raloxifene on human endometrial stromal cells. Methods: This experimental study was performed in 2019 on 16 samples of endometrial biopsy of women with normal menstrual cycles in secretory phase. Endometrial biopsies were digested by collagenase type I. Stromal cells were harvested by using serial filtration of cell suspension through cell strainer (70 and 40 µm) and ficoll layering. The cells were confirmed by immunohistochemical staining for vimentin. The cells of each biopsy were divided into: control and letrozole, or control and raloxifene. After 15 day, morphological changes and cell viability were determined. Data were analyzed by one way ANOVA and Tukey test. Results: Cell viability in control and letrozole 0.1, 1 and 10 µM concentration was 14.7, 7.8, 5.6 and 4.2 ×104 cells, respectively. Cell viability in control and raloxifene 0.1, 1 and 10 µM was 21.8, 12.9, 9.1 and 3×104 cells, respectively; the difference was significant (P<0.05). The morphological changes score of cells were 3.86, 3.38, 2.68 and 2.08 in control and letrozole groups, and were 3.98, 3.77, 3.72, 1.18 in control and raloxifene; the difference was significant (P<0.05). The effect of letrozole and raloxifene on the bioavailability and morphology of endometrial stromal cells was different. Conclusion: Letrozole and raloxifene showed anti-proliferative and dose-dependent effects on in vitro human endometrial stromal cells and raloxifene effect was more potent than letrozole.https://ijogi.mums.ac.ir/article_16611_44f6512b6e359aee28120348d0bc076f.pdfendometriumletrozoleraloxifenestromal cells |
| spellingShingle | Maryam Pourheydar Zahra Rashidi Mozafar Khazaei Effect of letrozole and raloxifene on in vitro human endometrial stromal cells Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān endometrium letrozole raloxifene stromal cells |
| title | Effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| title_full | Effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| title_fullStr | Effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| title_full_unstemmed | Effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| title_short | Effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| title_sort | effect of letrozole and raloxifene on in vitro human endometrial stromal cells |
| topic | endometrium letrozole raloxifene stromal cells |
| url | https://ijogi.mums.ac.ir/article_16611_44f6512b6e359aee28120348d0bc076f.pdf |
| work_keys_str_mv | AT maryampourheydar effectofletrozoleandraloxifeneoninvitrohumanendometrialstromalcells AT zahrarashidi effectofletrozoleandraloxifeneoninvitrohumanendometrialstromalcells AT mozafarkhazaei effectofletrozoleandraloxifeneoninvitrohumanendometrialstromalcells |