Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein
Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specifc mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in vario...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2023-01-01
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| Series: | Сибирский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.siboncoj.ru/jour/article/view/2378 |
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| author | N. A. Rahmah H. Harliansyah F. D. Suyatna M. Kanoko P. Rustamadji J. Prihartono A. Bustami S. J. Haryono B. S. Hernowo |
| author_facet | N. A. Rahmah H. Harliansyah F. D. Suyatna M. Kanoko P. Rustamadji J. Prihartono A. Bustami S. J. Haryono B. S. Hernowo |
| author_sort | N. A. Rahmah |
| collection | DOAJ |
| description | Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specifc mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types.This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells.Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using fow cytometry.Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) signifcantly.Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax. |
| first_indexed | 2024-04-10T01:52:08Z |
| format | Article |
| id | doaj.art-5f3333a9b0124319a47cd23677c82a6a |
| institution | Directory Open Access Journal |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| last_indexed | 2024-04-10T01:52:08Z |
| publishDate | 2023-01-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj.art-5f3333a9b0124319a47cd23677c82a6a2023-03-13T09:05:56ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682023-01-01216919810.21294/1814-4861-2022-21-6-91-981066Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 proteinN. A. Rahmah0H. Harliansyah1F. D. Suyatna2M. Kanoko3P. Rustamadji4J. Prihartono5A. Bustami6S. J. Haryono7B. S. Hernowo8Университет ЯрсиУниверситет ЯрсиУниверситет ИндонезииУниверситет ИндонезииУниверситет ИндонезииУниверситет ИндонезииУниверситет ИндонезииБольница Национального онкологического центра DharmaisУниверситет ПаджаджаранBackground. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specifc mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types.This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells.Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using fow cytometry.Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) signifcantly.Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.https://www.siboncoj.ru/jour/article/view/2378baxcaspase-3куркуминcsa03rassf1a |
| spellingShingle | N. A. Rahmah H. Harliansyah F. D. Suyatna M. Kanoko P. Rustamadji J. Prihartono A. Bustami S. J. Haryono B. S. Hernowo Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein Сибирский онкологический журнал bax caspase-3 куркумин csa03 rassf1a |
| title | Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein |
| title_full | Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein |
| title_fullStr | Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein |
| title_full_unstemmed | Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein |
| title_short | Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 protein |
| title_sort | curcumin as an anti proliferative agent in breast cancer through rassf1a bax and caspase 3 protein |
| topic | bax caspase-3 куркумин csa03 rassf1a |
| url | https://www.siboncoj.ru/jour/article/view/2378 |
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