The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research
Cardiac fibroblasts’ (FBs) and cardiomyocytes’ (CMs) behaviour and morphology are influenced by their environment such as remodelling of the myocardium, thus highlighting the importance of biomaterial substrates in cell culture. Biomaterials have emerged as important tools for the development of phy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/9995 |
| _version_ | 1797594352563781632 |
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| author | Leena Patel Joshua C. Worch Andrew P. Dove Katja Gehmlich |
| author_facet | Leena Patel Joshua C. Worch Andrew P. Dove Katja Gehmlich |
| author_sort | Leena Patel |
| collection | DOAJ |
| description | Cardiac fibroblasts’ (FBs) and cardiomyocytes’ (CMs) behaviour and morphology are influenced by their environment such as remodelling of the myocardium, thus highlighting the importance of biomaterial substrates in cell culture. Biomaterials have emerged as important tools for the development of physiological models, due to the range of adaptable properties of these materials, such as degradability and biocompatibility. Biomaterial hydrogels can act as alternative substrates for cellular studies, which have been particularly key to the progression of the cardiovascular field. This review will focus on the role of hydrogels in cardiac research, specifically the use of natural and synthetic biomaterials such as hyaluronic acid, polydimethylsiloxane and polyethylene glycol for culturing induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). The ability to fine-tune mechanical properties such as stiffness and the versatility of biomaterials is assessed, alongside applications of hydrogels with iPSC-CMs. Natural hydrogels often display higher biocompatibility with iPSC-CMs but often degrade quicker, whereas synthetic hydrogels can be modified to facilitate cell attachment and decrease degradation rates. iPSC-CM structure and electrophysiology can be assessed on natural and synthetic hydrogels, often resolving issues such as immaturity of iPSC-CMs. Biomaterial hydrogels can thus provide a more physiological model of the cardiac extracellular matrix compared to traditional 2D models, with the cardiac field expansively utilising hydrogels to recapitulate disease conditions such as stiffness, encourage alignment of iPSC-CMs and facilitate further model development such as engineered heart tissues (EHTs). |
| first_indexed | 2024-03-11T02:21:21Z |
| format | Article |
| id | doaj.art-5f37852f3bd747f0a17b0db1878fc78d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:21:21Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f37852f3bd747f0a17b0db1878fc78d2023-11-18T10:47:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012412999510.3390/ijms24129995The Utilisation of Hydrogels for iPSC-Cardiomyocyte ResearchLeena Patel0Joshua C. Worch1Andrew P. Dove2Katja Gehmlich3Institute of Cardiovascular Science, University of Birmingham, Birmingham B15 2TT, UKSchool of Chemistry, University of Birmingham, Birmingham B15 2TT, UKSchool of Chemistry, University of Birmingham, Birmingham B15 2TT, UKInstitute of Cardiovascular Science, University of Birmingham, Birmingham B15 2TT, UKCardiac fibroblasts’ (FBs) and cardiomyocytes’ (CMs) behaviour and morphology are influenced by their environment such as remodelling of the myocardium, thus highlighting the importance of biomaterial substrates in cell culture. Biomaterials have emerged as important tools for the development of physiological models, due to the range of adaptable properties of these materials, such as degradability and biocompatibility. Biomaterial hydrogels can act as alternative substrates for cellular studies, which have been particularly key to the progression of the cardiovascular field. This review will focus on the role of hydrogels in cardiac research, specifically the use of natural and synthetic biomaterials such as hyaluronic acid, polydimethylsiloxane and polyethylene glycol for culturing induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). The ability to fine-tune mechanical properties such as stiffness and the versatility of biomaterials is assessed, alongside applications of hydrogels with iPSC-CMs. Natural hydrogels often display higher biocompatibility with iPSC-CMs but often degrade quicker, whereas synthetic hydrogels can be modified to facilitate cell attachment and decrease degradation rates. iPSC-CM structure and electrophysiology can be assessed on natural and synthetic hydrogels, often resolving issues such as immaturity of iPSC-CMs. Biomaterial hydrogels can thus provide a more physiological model of the cardiac extracellular matrix compared to traditional 2D models, with the cardiac field expansively utilising hydrogels to recapitulate disease conditions such as stiffness, encourage alignment of iPSC-CMs and facilitate further model development such as engineered heart tissues (EHTs).https://www.mdpi.com/1422-0067/24/12/9995extracellular matrixhydrogelsbiomaterialsstiffnesscardiovascular |
| spellingShingle | Leena Patel Joshua C. Worch Andrew P. Dove Katja Gehmlich The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research International Journal of Molecular Sciences extracellular matrix hydrogels biomaterials stiffness cardiovascular |
| title | The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research |
| title_full | The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research |
| title_fullStr | The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research |
| title_full_unstemmed | The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research |
| title_short | The Utilisation of Hydrogels for iPSC-Cardiomyocyte Research |
| title_sort | utilisation of hydrogels for ipsc cardiomyocyte research |
| topic | extracellular matrix hydrogels biomaterials stiffness cardiovascular |
| url | https://www.mdpi.com/1422-0067/24/12/9995 |
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