Study of the inhibitory effect of some thiourea compounds on the activity of enzyme purified polyamine oxidase from the blood of patients with type 1 diabetes
<strong>ABSTRACT</strong><br /> The research included determination of polyamine oxidase (PAO) activity in normal and diabeti patients type I females. It was found that the activity of PAO in red blood cells (RBC) and plasma in patients were significantly higher than that of nor...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Arabic |
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College of Education for Pure Sciences
2013-12-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_163091_d63b5cfa1f267ef1079b4e8ad94081e6.pdf |
| Summary: | <strong>ABSTRACT</strong><br /> The research included determination of polyamine oxidase (PAO) activity in normal and diabeti patients type I females. It was found that the activity of PAO in red blood cells (RBC) and plasma in patients were significantly higher than that of normal(p<0.05).<br /> The partial purification of PAO from RBC of diabetic and normal females were included in this study. This was achieved by using different biochemical techniques. Three proteinous peaks with PAO activities in RBC (I,II,III) from each of normal and diabetic with specific activities (Umg protein) (0.194,0.183 and 0.098), (0.349, 0.237 and 0.176) respectively were isolated from ion exchange chromatography, with molecular weights (72588, 74512 and 69339), (141458, 100671 and 104018) Da respectively.<br /> This study didn't show the existence of Cu<sup>2+</sup> ion as a cofactor for any PAO isoenzymes, but indicated the existence of FAD as a cofactor for all PAO isoenzymes.<br /> On the other hand the research included study the effect of some thiourea derivatives which contains semicarbazide compound , N[(Benzoylamino)]thioxymethyl-semicarbazide and N[(<em>p-</em>toluine sulfonylamino)] thioxymethyl-semicarbazide, and N[(<em>p-</em>toluine sulfonylamino)] thioxymethyl-hexylamine which contained hexylamine . Semicarbazide showed inhibitory effect on partially purified PAO activity. Thiourea derivatives from Semicarbazide showed competitive inhibition of PAO activity but with hexylamine was non- competitive. |
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| ISSN: | 1812-125X 2664-2530 |