Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation
We report, for the first time, the full-field 3D strain distribution of the muscle-tendon junction (MTJ). Understanding the strain distribution at the junction is crucial for the treatment of injuries and to predict tear formation at this location. Three-dimensional full-field strain distribution of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/11/2/162 |
| _version_ | 1797298888816721920 |
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| author | Nodoka Iwasaki Aikaterina Karali Marta Roldo Gordon Blunn |
| author_facet | Nodoka Iwasaki Aikaterina Karali Marta Roldo Gordon Blunn |
| author_sort | Nodoka Iwasaki |
| collection | DOAJ |
| description | We report, for the first time, the full-field 3D strain distribution of the muscle-tendon junction (MTJ). Understanding the strain distribution at the junction is crucial for the treatment of injuries and to predict tear formation at this location. Three-dimensional full-field strain distribution of mouse MTJ was measured using X-ray computer tomography (XCT) combined with digital volume correlation (DVC) with the aim of understanding the mechanical behavior of the junction under tensile loading. The interface between the Achilles tendon and the gastrocnemius muscle was harvested from adult mice and stained using 1% phosphotungstic acid in 70% ethanol. In situ XCT combined with DVC was used to image and compute strain distribution at the MTJ under a tensile load (2.4 N). High strain measuring 120,000 µε, 160,000 µε, and 120,000 µε for the first principal stain (ε<sub>p1</sub>), shear strain (γ), and von Mises strain (ε<sub>VM</sub>), respectively, was measured at the MTJ and these values reduced into the body of the muscle or into the tendon. Strain is concentrated at the MTJ, which is at risk of being damaged in activities associated with excessive physical activity. |
| first_indexed | 2024-03-07T22:41:39Z |
| format | Article |
| id | doaj.art-5f3a64fb7b434caf823366b9e4a69b68 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-07T22:41:39Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-5f3a64fb7b434caf823366b9e4a69b682024-02-23T15:07:59ZengMDPI AGBioengineering2306-53542024-02-0111216210.3390/bioengineering11020162Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume CorrelationNodoka Iwasaki0Aikaterina Karali1Marta Roldo2Gordon Blunn3School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UKSchool of Mechanical and Design Engineering, University of Portsmouth, Portsmouth PO1 3DJ, UKSchool of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UKSchool of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UKWe report, for the first time, the full-field 3D strain distribution of the muscle-tendon junction (MTJ). Understanding the strain distribution at the junction is crucial for the treatment of injuries and to predict tear formation at this location. Three-dimensional full-field strain distribution of mouse MTJ was measured using X-ray computer tomography (XCT) combined with digital volume correlation (DVC) with the aim of understanding the mechanical behavior of the junction under tensile loading. The interface between the Achilles tendon and the gastrocnemius muscle was harvested from adult mice and stained using 1% phosphotungstic acid in 70% ethanol. In situ XCT combined with DVC was used to image and compute strain distribution at the MTJ under a tensile load (2.4 N). High strain measuring 120,000 µε, 160,000 µε, and 120,000 µε for the first principal stain (ε<sub>p1</sub>), shear strain (γ), and von Mises strain (ε<sub>VM</sub>), respectively, was measured at the MTJ and these values reduced into the body of the muscle or into the tendon. Strain is concentrated at the MTJ, which is at risk of being damaged in activities associated with excessive physical activity.https://www.mdpi.com/2306-5354/11/2/162strain3D strain measurementmuscle–tendon junction (MTJ)X-ray computed tomography (XCT)digital volume correlation (DVC)in situ mechanical testing |
| spellingShingle | Nodoka Iwasaki Aikaterina Karali Marta Roldo Gordon Blunn Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation Bioengineering strain 3D strain measurement muscle–tendon junction (MTJ) X-ray computed tomography (XCT) digital volume correlation (DVC) in situ mechanical testing |
| title | Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation |
| title_full | Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation |
| title_fullStr | Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation |
| title_full_unstemmed | Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation |
| title_short | Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation |
| title_sort | full field strain measurements of the muscle tendon junction using x ray computed tomography and digital volume correlation |
| topic | strain 3D strain measurement muscle–tendon junction (MTJ) X-ray computed tomography (XCT) digital volume correlation (DVC) in situ mechanical testing |
| url | https://www.mdpi.com/2306-5354/11/2/162 |
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