Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond
Signal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes. Signal peptides play a significant role in recombinant protein production. Previous studies have demonstrated that...
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00139/full |
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| author | Chong Peng Chong Peng Chong Peng Chong Peng Chaoshuo Shi Xue Cao Yu Li Yu Li Yu Li Yu Li Fufeng Liu Fufeng Liu Fufeng Liu Fufeng Liu Fuping Lu Fuping Lu Fuping Lu Fuping Lu |
| author_facet | Chong Peng Chong Peng Chong Peng Chong Peng Chaoshuo Shi Xue Cao Yu Li Yu Li Yu Li Yu Li Fufeng Liu Fufeng Liu Fufeng Liu Fufeng Liu Fuping Lu Fuping Lu Fuping Lu Fuping Lu |
| author_sort | Chong Peng |
| collection | DOAJ |
| description | Signal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes. Signal peptides play a significant role in recombinant protein production. Previous studies have demonstrated that the secretion amount of a given target protein varies significantly depending on the signal peptide that is fused to the protein. Signal peptide selection and signal peptide modification are the two main methods for the optimization of a recombinant protein secretion. However, the highly efficient signal peptide for a target protein with a specific bacterial expression host is not predictable so far. In this article, we collect several signal peptides that have previously performed well for recombinant protein secretion in gram-positive bacteria. We also discuss several factors influencing recombinant protein secretion efficiency in gram-positive bacteria. Signal peptides with a higher charge/length ratio in n-region, more consensus residues at the−3 and−1positions in c-region and a much higher proportion of coils are more likely to perform well in the secretion of recombinant proteins. These summaries can be utilized to the selection and directed modification of signal peptides for a given recombinant protein. |
| first_indexed | 2024-12-13T04:16:45Z |
| format | Article |
| id | doaj.art-5f3c14a5202e4101b26517c044facb45 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T04:16:45Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-5f3c14a5202e4101b26517c044facb452022-12-21T23:59:53ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-06-01710.3389/fbioe.2019.00139448926Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and BeyondChong Peng0Chong Peng1Chong Peng2Chong Peng3Chaoshuo Shi4Xue Cao5Yu Li6Yu Li7Yu Li8Yu Li9Fufeng Liu10Fufeng Liu11Fufeng Liu12Fufeng Liu13Fuping Lu14Fuping Lu15Fuping Lu16Fuping Lu17Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, Tianjin, ChinaNational Engineering Laboratory for Industrial Enzymes, Tianjin, ChinaTianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaKey Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, Tianjin, ChinaNational Engineering Laboratory for Industrial Enzymes, Tianjin, ChinaTianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaKey Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, Tianjin, ChinaNational Engineering Laboratory for Industrial Enzymes, Tianjin, ChinaTianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaKey Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, Tianjin, ChinaNational Engineering Laboratory for Industrial Enzymes, Tianjin, ChinaTianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaSignal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes. Signal peptides play a significant role in recombinant protein production. Previous studies have demonstrated that the secretion amount of a given target protein varies significantly depending on the signal peptide that is fused to the protein. Signal peptide selection and signal peptide modification are the two main methods for the optimization of a recombinant protein secretion. However, the highly efficient signal peptide for a target protein with a specific bacterial expression host is not predictable so far. In this article, we collect several signal peptides that have previously performed well for recombinant protein secretion in gram-positive bacteria. We also discuss several factors influencing recombinant protein secretion efficiency in gram-positive bacteria. Signal peptides with a higher charge/length ratio in n-region, more consensus residues at the−3 and−1positions in c-region and a much higher proportion of coils are more likely to perform well in the secretion of recombinant proteins. These summaries can be utilized to the selection and directed modification of signal peptides for a given recombinant protein.https://www.frontiersin.org/article/10.3389/fbioe.2019.00139/fullsignal peptiderecombinant proteinsecretory pathwaygram-positive bacteriasecretion efficiency |
| spellingShingle | Chong Peng Chong Peng Chong Peng Chong Peng Chaoshuo Shi Xue Cao Yu Li Yu Li Yu Li Yu Li Fufeng Liu Fufeng Liu Fufeng Liu Fufeng Liu Fuping Lu Fuping Lu Fuping Lu Fuping Lu Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond Frontiers in Bioengineering and Biotechnology signal peptide recombinant protein secretory pathway gram-positive bacteria secretion efficiency |
| title | Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond |
| title_full | Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond |
| title_fullStr | Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond |
| title_full_unstemmed | Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond |
| title_short | Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond |
| title_sort | factors influencing recombinant protein secretion efficiency in gram positive bacteria signal peptide and beyond |
| topic | signal peptide recombinant protein secretory pathway gram-positive bacteria secretion efficiency |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00139/full |
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