Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway
In China, the national-level protected pig, the Min pig, is characterized by the development of secondary hairs and hair follicles in winter. Factors that dominate the genotype in the growth of secondary hairs are not clear through the concrete cell signaling pathways. This study compared hair pheno...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/7/1239 |
| _version_ | 1827747130342637568 |
|---|---|
| author | Xinmiao He Ziliang Qin Ran Teng Ming Tian Wentao Wang Yanzhong Feng Heshu Chen Haijuan He Haifeng Zhang Di Liu Xinpeng Jiang |
| author_facet | Xinmiao He Ziliang Qin Ran Teng Ming Tian Wentao Wang Yanzhong Feng Heshu Chen Haijuan He Haifeng Zhang Di Liu Xinpeng Jiang |
| author_sort | Xinmiao He |
| collection | DOAJ |
| description | In China, the national-level protected pig, the Min pig, is characterized by the development of secondary hairs and hair follicles in winter. Factors that dominate the genotype in the growth of secondary hairs are not clear through the concrete cell signaling pathways. This study compared hair phenotypes based on morphological structure, transcriptomics, and potential targeting molecules in the breeds of Min, Berkshire, and Yorkshire pigs. The results indicated that Min pigs have specific characteristics for the growth of secondary hairs compared with the Berkshire and Yorkshire pigs. The transcriptome analyses and quantitative reverse transcription-polymerase chain reaction results revealed that secondary hair growth was activated by follicle stem cells. The specific inhibitors of Wnt and BMP were studied using respective signals. The density of follicles, activity of follicle stem cells, and relative gene expression results have shown that Wnt and BMP stimulate the activity of follicle stem cells, and the Wnt signaling molecule has a significantly better effect than the BMP signaling molecule on stem cells. Wnt and BMP can promote the growth of local secondary hair and gene expression. Therefore, this study was conducted to verify the development mechanisms of secondary hairs, which have potential applications in laboratory animals and comparative medicine. |
| first_indexed | 2024-03-11T05:43:46Z |
| format | Article |
| id | doaj.art-5f3c3faf4c554e3788957a26bedfdc1e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:43:46Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5f3c3faf4c554e3788957a26bedfdc1e2023-11-17T16:14:41ZengMDPI AGAnimals2076-26152023-04-01137123910.3390/ani13071239Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling PathwayXinmiao He0Ziliang Qin1Ran Teng2Ming Tian3Wentao Wang4Yanzhong Feng5Heshu Chen6Haijuan He7Haifeng Zhang8Di Liu9Xinpeng Jiang10Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture and Rural Affairs, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Harbin 150086, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaIn China, the national-level protected pig, the Min pig, is characterized by the development of secondary hairs and hair follicles in winter. Factors that dominate the genotype in the growth of secondary hairs are not clear through the concrete cell signaling pathways. This study compared hair phenotypes based on morphological structure, transcriptomics, and potential targeting molecules in the breeds of Min, Berkshire, and Yorkshire pigs. The results indicated that Min pigs have specific characteristics for the growth of secondary hairs compared with the Berkshire and Yorkshire pigs. The transcriptome analyses and quantitative reverse transcription-polymerase chain reaction results revealed that secondary hair growth was activated by follicle stem cells. The specific inhibitors of Wnt and BMP were studied using respective signals. The density of follicles, activity of follicle stem cells, and relative gene expression results have shown that Wnt and BMP stimulate the activity of follicle stem cells, and the Wnt signaling molecule has a significantly better effect than the BMP signaling molecule on stem cells. Wnt and BMP can promote the growth of local secondary hair and gene expression. Therefore, this study was conducted to verify the development mechanisms of secondary hairs, which have potential applications in laboratory animals and comparative medicine.https://www.mdpi.com/2076-2615/13/7/1239Min pigfollicle stem cellBMPWnt |
| spellingShingle | Xinmiao He Ziliang Qin Ran Teng Ming Tian Wentao Wang Yanzhong Feng Heshu Chen Haijuan He Haifeng Zhang Di Liu Xinpeng Jiang Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway Animals Min pig follicle stem cell BMP Wnt |
| title | Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway |
| title_full | Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway |
| title_fullStr | Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway |
| title_full_unstemmed | Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway |
| title_short | Characterization of Growth Secondary Hair in Min Pig Activated by Follicle Stem Cell Stimulated by Wnt and BMP Signaling Pathway |
| title_sort | characterization of growth secondary hair in min pig activated by follicle stem cell stimulated by wnt and bmp signaling pathway |
| topic | Min pig follicle stem cell BMP Wnt |
| url | https://www.mdpi.com/2076-2615/13/7/1239 |
| work_keys_str_mv | AT xinmiaohe characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT ziliangqin characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT ranteng characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT mingtian characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT wentaowang characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT yanzhongfeng characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT heshuchen characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT haijuanhe characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT haifengzhang characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT diliu characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway AT xinpengjiang characterizationofgrowthsecondaryhairinminpigactivatedbyfolliclestemcellstimulatedbywntandbmpsignalingpathway |