Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector
BACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which c...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2018-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018001200302&lng=en&tlng=en |
| _version_ | 1797713487198158848 |
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| author | Annhymariet Torrellas Elizabeth Ferrer Israel Cruz Héctor de Lima Olinda Delgado José Carrero Rangel José Arturo Bravo Carmen Chicharro Ivonne Pamela Llanes-Acevedo Michael A Miles María Dora Feliciangeli |
| author_facet | Annhymariet Torrellas Elizabeth Ferrer Israel Cruz Héctor de Lima Olinda Delgado José Carrero Rangel José Arturo Bravo Carmen Chicharro Ivonne Pamela Llanes-Acevedo Michael A Miles María Dora Feliciangeli |
| author_sort | Annhymariet Torrellas |
| collection | DOAJ |
| description | BACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which causes ACL in the Andean Region of Venezuela, by identifying the Leishmania and the sand fly species involved in human and dog infections. METHODS Thirty-one biopsies from patients in Mérida and Táchira states with suspected ACL were studied by both parasitological tests (cultures and hamster inoculation) and a molecular test [Internal transcribed spacer 1 (ITS1) nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)]. We also conducted a survey to detect Leishmania infection in dogs (Immunifluorescence antibody test and ITS1 nested PCR-RFLP) and sand flies (ITS1 nested PCR-RFLP) from El Carrizal, a highly endemic focus of ACL in Venezuela. FINDINGS Three different Leishmania species were identified in the clinical samples from humans (Leishmania braziliensis, L. guyanensis, and L. mexicana) and dogs (L. guyanensis and L. mexicana). The predominant sand fly species found were those from the Verrucarum group (infected with L. mexicana) and Lutzomyia migonei (infected with L. guyanensis and L. mexicana). MAIN CONCLUSIONS We show that Lu. migonei may be the putative vector in two ACL epidemiological cycles, involving L. guyanensis and L. mexicana. We also report for the first time the presence of L. guyanensis in domestic animals. |
| first_indexed | 2024-03-12T07:37:20Z |
| format | Article |
| id | doaj.art-5f3f15022b74420599a75bcc3a8a9452 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:37:20Z |
| publishDate | 2018-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-5f3f15022b74420599a75bcc3a8a94522023-09-02T21:28:08ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602018-12-011131210.1590/0074-02760180323S0074-02762018001200302Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vectorAnnhymariet TorrellasElizabeth FerrerIsrael CruzHéctor de LimaOlinda DelgadoJosé Carrero RangelJosé Arturo BravoCarmen ChicharroIvonne Pamela Llanes-AcevedoMichael A MilesMaría Dora FeliciangeliBACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which causes ACL in the Andean Region of Venezuela, by identifying the Leishmania and the sand fly species involved in human and dog infections. METHODS Thirty-one biopsies from patients in Mérida and Táchira states with suspected ACL were studied by both parasitological tests (cultures and hamster inoculation) and a molecular test [Internal transcribed spacer 1 (ITS1) nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)]. We also conducted a survey to detect Leishmania infection in dogs (Immunifluorescence antibody test and ITS1 nested PCR-RFLP) and sand flies (ITS1 nested PCR-RFLP) from El Carrizal, a highly endemic focus of ACL in Venezuela. FINDINGS Three different Leishmania species were identified in the clinical samples from humans (Leishmania braziliensis, L. guyanensis, and L. mexicana) and dogs (L. guyanensis and L. mexicana). The predominant sand fly species found were those from the Verrucarum group (infected with L. mexicana) and Lutzomyia migonei (infected with L. guyanensis and L. mexicana). MAIN CONCLUSIONS We show that Lu. migonei may be the putative vector in two ACL epidemiological cycles, involving L. guyanensis and L. mexicana. We also report for the first time the presence of L. guyanensis in domestic animals.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018001200302&lng=en&tlng=enLeishmaniaepidemiologydiagnosisPCR-RFLP |
| spellingShingle | Annhymariet Torrellas Elizabeth Ferrer Israel Cruz Héctor de Lima Olinda Delgado José Carrero Rangel José Arturo Bravo Carmen Chicharro Ivonne Pamela Llanes-Acevedo Michael A Miles María Dora Feliciangeli Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector Memorias do Instituto Oswaldo Cruz Leishmania epidemiology diagnosis PCR-RFLP |
| title | Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector |
| title_full | Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector |
| title_fullStr | Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector |
| title_full_unstemmed | Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector |
| title_short | Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector |
| title_sort | molecular typing reveals the co existence of two transmission cycles of american cutaneous leishmaniasis in the andean region of venezuela with lutzomyia migonei as the vector |
| topic | Leishmania epidemiology diagnosis PCR-RFLP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018001200302&lng=en&tlng=en |
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