DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.
Norwegian lobster, Nephrops norvegicus, are a generalist scavenger and predator capable of short foraging excursions but can also suspension feed. Existing knowledge about their diet relies on a combination of methods including morphology-based stomach content analysis and stable isotopes, which oft...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0289221&type=printable |
| _version_ | 1797635190782164992 |
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| author | Peter Shum Janine Wäge-Recchioni Graham S Sellers Magnus L Johnson Domino A Joyce |
| author_facet | Peter Shum Janine Wäge-Recchioni Graham S Sellers Magnus L Johnson Domino A Joyce |
| author_sort | Peter Shum |
| collection | DOAJ |
| description | Norwegian lobster, Nephrops norvegicus, are a generalist scavenger and predator capable of short foraging excursions but can also suspension feed. Existing knowledge about their diet relies on a combination of methods including morphology-based stomach content analysis and stable isotopes, which often lack the resolution to distinguish prey items to species level particularly in species that thoroughly masticate their prey. DNA metabarcoding overcomes many of the challenges associated with traditional methods and it is an attractive approach to study the dietary profiles of animals. Here, we present the diet of the commercially valuable Nephrops norvegicus using DNA metabarcoding of gut contents. Despite difficulties associated with host amplification, our cytochrome oxidase I (COI) molecular assay successfully achieves higher resolution information than traditional approaches. We detected taxa that were likely consumed during different feeding strategies. Dinoflagellata, Chlorophyta and Bacillariophyta accounted for almost 50% of the prey items consumed, and are associated with suspension feeding, while fish with high fisheries discard rates were detected which are linked to active foraging. In addition, we were able to characterise biodiversity patterns by considering Nephrops as natural samplers, as well as detecting parasitic dinoflagellates (e.g., Hematodinium sp.), which are known to influence burrow related behaviour in infected individuals in over 50% of the samples. The metabarcoding data presented here greatly enhances a better understanding of a species' ecological role and could be applied as a routine procedure in future studies for proper consideration in the management and decision-making of fisheries. |
| first_indexed | 2024-03-11T12:18:41Z |
| format | Article |
| id | doaj.art-5f3ff54302ab4388a3519f9d91d9a11e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T12:18:41Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5f3ff54302ab4388a3519f9d91d9a11e2023-11-07T05:34:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011811e028922110.1371/journal.pone.0289221DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.Peter ShumJanine Wäge-RecchioniGraham S SellersMagnus L JohnsonDomino A JoyceNorwegian lobster, Nephrops norvegicus, are a generalist scavenger and predator capable of short foraging excursions but can also suspension feed. Existing knowledge about their diet relies on a combination of methods including morphology-based stomach content analysis and stable isotopes, which often lack the resolution to distinguish prey items to species level particularly in species that thoroughly masticate their prey. DNA metabarcoding overcomes many of the challenges associated with traditional methods and it is an attractive approach to study the dietary profiles of animals. Here, we present the diet of the commercially valuable Nephrops norvegicus using DNA metabarcoding of gut contents. Despite difficulties associated with host amplification, our cytochrome oxidase I (COI) molecular assay successfully achieves higher resolution information than traditional approaches. We detected taxa that were likely consumed during different feeding strategies. Dinoflagellata, Chlorophyta and Bacillariophyta accounted for almost 50% of the prey items consumed, and are associated with suspension feeding, while fish with high fisheries discard rates were detected which are linked to active foraging. In addition, we were able to characterise biodiversity patterns by considering Nephrops as natural samplers, as well as detecting parasitic dinoflagellates (e.g., Hematodinium sp.), which are known to influence burrow related behaviour in infected individuals in over 50% of the samples. The metabarcoding data presented here greatly enhances a better understanding of a species' ecological role and could be applied as a routine procedure in future studies for proper consideration in the management and decision-making of fisheries.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0289221&type=printable |
| spellingShingle | Peter Shum Janine Wäge-Recchioni Graham S Sellers Magnus L Johnson Domino A Joyce DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. PLoS ONE |
| title | DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. |
| title_full | DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. |
| title_fullStr | DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. |
| title_full_unstemmed | DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. |
| title_short | DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. |
| title_sort | dna metabarcoding reveals the dietary profiles of a benthic marine crustacean nephrops norvegicus |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0289221&type=printable |
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