What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany
The aim of this study was to gain an overview of the genetic diversity of <i>Salmonella</i> found in wildlife in Germany. We were particularly interested in exploring whether wildlife acts as a reservoir of certain serovars/subtypes or antimicrobial resistance (AMR) genes. Moreover, we w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1911 |
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| author | Laura Uelze Angelina Bloch Maria Borowiak Mirjam Grobbel Carlus Deneke Matthias Fischer Burkhard Malorny Michael Pietsch Sandra Simon István Szabó Simon H. Tausch Jennie Fischer |
| author_facet | Laura Uelze Angelina Bloch Maria Borowiak Mirjam Grobbel Carlus Deneke Matthias Fischer Burkhard Malorny Michael Pietsch Sandra Simon István Szabó Simon H. Tausch Jennie Fischer |
| author_sort | Laura Uelze |
| collection | DOAJ |
| description | The aim of this study was to gain an overview of the genetic diversity of <i>Salmonella</i> found in wildlife in Germany. We were particularly interested in exploring whether wildlife acts as a reservoir of certain serovars/subtypes or antimicrobial resistance (AMR) genes. Moreover, we wanted to explore the potential of <i>Salmonella</i> in spreading from wildlife to livestock and humans. To answer these questions, we sequenced 260 <i>Salmonella enterica</i> subsp. <i>enterica</i> isolates sampled between 2002 and 2020 from wildlife across Germany, using short-read whole genome sequencing. We found, consistent with previous findings, that some <i>Salmonella</i> sequence types are associated with certain animal species, such as <i>S.</i> Choleraesuis ST145 with wild boar and <i>S.</i> Enteritidis ST183 with hedgehogs. Antibiotic resistance was detected in 14.2% of all isolates, with resistance against important WATCH group antibiotics present in a small number of isolates. We further found that wildlife isolates do not form separate phylogenetic clusters distant to isolates from domestic animals and foodstuff, thus indicating frequent transmission events between these reservoirs. Overall, our study shows that <i>Salmonella</i> in German wildlife are diverse, with a low AMR burden and close links to <i>Salmonella</i> populations of farm and food-production environments. |
| first_indexed | 2024-03-10T07:25:05Z |
| format | Article |
| id | doaj.art-5f44aff2ef7f480982139e37f59bbf47 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:05Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5f44aff2ef7f480982139e37f59bbf472023-11-22T14:18:59ZengMDPI AGMicroorganisms2076-26072021-09-0199191110.3390/microorganisms9091911What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in GermanyLaura Uelze0Angelina Bloch1Maria Borowiak2Mirjam Grobbel3Carlus Deneke4Matthias Fischer5Burkhard Malorny6Michael Pietsch7Sandra Simon8István Szabó9Simon H. Tausch10Jennie Fischer11Department of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyUnit for Enteropathogenic Bacteria and Legionella (FG11)/National Reference Centre for Salmonella and Other Bacterial Enteric Pathogens, Robert Koch Institute (RKI), Burgstr. 37, 38855 Wernigerode, GermanyUnit for Enteropathogenic Bacteria and Legionella (FG11)/National Reference Centre for Salmonella and Other Bacterial Enteric Pathogens, Robert Koch Institute (RKI), Burgstr. 37, 38855 Wernigerode, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, GermanyThe aim of this study was to gain an overview of the genetic diversity of <i>Salmonella</i> found in wildlife in Germany. We were particularly interested in exploring whether wildlife acts as a reservoir of certain serovars/subtypes or antimicrobial resistance (AMR) genes. Moreover, we wanted to explore the potential of <i>Salmonella</i> in spreading from wildlife to livestock and humans. To answer these questions, we sequenced 260 <i>Salmonella enterica</i> subsp. <i>enterica</i> isolates sampled between 2002 and 2020 from wildlife across Germany, using short-read whole genome sequencing. We found, consistent with previous findings, that some <i>Salmonella</i> sequence types are associated with certain animal species, such as <i>S.</i> Choleraesuis ST145 with wild boar and <i>S.</i> Enteritidis ST183 with hedgehogs. Antibiotic resistance was detected in 14.2% of all isolates, with resistance against important WATCH group antibiotics present in a small number of isolates. We further found that wildlife isolates do not form separate phylogenetic clusters distant to isolates from domestic animals and foodstuff, thus indicating frequent transmission events between these reservoirs. Overall, our study shows that <i>Salmonella</i> in German wildlife are diverse, with a low AMR burden and close links to <i>Salmonella</i> populations of farm and food-production environments.https://www.mdpi.com/2076-2607/9/9/1911<i>Salmonella enterica</i> subsp. <i>enterica</i>whole-genome sequencingwildlife |
| spellingShingle | Laura Uelze Angelina Bloch Maria Borowiak Mirjam Grobbel Carlus Deneke Matthias Fischer Burkhard Malorny Michael Pietsch Sandra Simon István Szabó Simon H. Tausch Jennie Fischer What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany Microorganisms <i>Salmonella enterica</i> subsp. <i>enterica</i> whole-genome sequencing wildlife |
| title | What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany |
| title_full | What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany |
| title_fullStr | What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany |
| title_full_unstemmed | What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany |
| title_short | What WGS Reveals about <i>Salmonella enterica</i> subsp. <i>enterica</i> in Wildlife in Germany |
| title_sort | what wgs reveals about i salmonella enterica i subsp i enterica i in wildlife in germany |
| topic | <i>Salmonella enterica</i> subsp. <i>enterica</i> whole-genome sequencing wildlife |
| url | https://www.mdpi.com/2076-2607/9/9/1911 |
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