Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses
Phosphate-based glasses (PBGs) offer significant therapeutic potential due to their bioactivity, controllable compositions, and degradation rates. Several PBGs have already demonstrated their ability to support direct cell growth and in vivo cytocompatibility for bone repair applications. This study...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/837 |
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| author | Nigel De Melo Lauren Murrell Md Towhidul Islam Jeremy J. Titman Laura Macri-Pellizzeri Ifty Ahmed Virginie Sottile |
| author_facet | Nigel De Melo Lauren Murrell Md Towhidul Islam Jeremy J. Titman Laura Macri-Pellizzeri Ifty Ahmed Virginie Sottile |
| author_sort | Nigel De Melo |
| collection | DOAJ |
| description | Phosphate-based glasses (PBGs) offer significant therapeutic potential due to their bioactivity, controllable compositions, and degradation rates. Several PBGs have already demonstrated their ability to support direct cell growth and in vivo cytocompatibility for bone repair applications. This study investigated development of PBG formulations with pyro- and orthophosphate species within the glass system (40 − x)P<sub>2</sub>O<sub>5</sub>·(16 + x)CaO·20Na<sub>2</sub>O·24MgO (x = 0, 5, 10 mol%) and their effect on stem cell adhesion properties. Substitution of phosphate for calcium revealed a gradual transition within the glass structure from Q<sup>2</sup> to Q<sup>0</sup> phosphate species. Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. Analysis of cells seeded onto the discs revealed that PBG with higher concentration of pyro- and orthophosphate content (61% Q<sup>1</sup> and 39% Q<sup>0</sup>) supported a 4.3-fold increase in adhered cells compared to glasses with metaphosphate connectivity (49% Q<sup>2</sup> and 51% Q<sup>1</sup>). This study highlights that tuning the composition of PBGs to possess pyro- and orthophosphate species only, enables the possibility to control cell adhesion performance. PBGs with superior cell adhesion profiles represent ideal candidates for biomedical applications, where cell recruitment and support for tissue ingrowth are of critical importance for orthopaedic interventions. |
| first_indexed | 2024-03-09T04:38:03Z |
| format | Article |
| id | doaj.art-5f45933e57dd44c3abf17ce083f73522 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:38:03Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f45933e57dd44c3abf17ce083f735222023-12-03T13:25:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122283710.3390/ijms22020837Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate GlassesNigel De Melo0Lauren Murrell1Md Towhidul Islam2Jeremy J. Titman3Laura Macri-Pellizzeri4Ifty Ahmed5Virginie Sottile6School of Medicine, University of Nottingham, Nottingham NG7 2RD, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UKSchool of Chemistry, University of Nottingham, Nottingham NG7 2RD, UKSchool of Medicine, University of Nottingham, Nottingham NG7 2RD, UKAdvanced Materials Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UKSchool of Medicine, University of Nottingham, Nottingham NG7 2RD, UKPhosphate-based glasses (PBGs) offer significant therapeutic potential due to their bioactivity, controllable compositions, and degradation rates. Several PBGs have already demonstrated their ability to support direct cell growth and in vivo cytocompatibility for bone repair applications. This study investigated development of PBG formulations with pyro- and orthophosphate species within the glass system (40 − x)P<sub>2</sub>O<sub>5</sub>·(16 + x)CaO·20Na<sub>2</sub>O·24MgO (x = 0, 5, 10 mol%) and their effect on stem cell adhesion properties. Substitution of phosphate for calcium revealed a gradual transition within the glass structure from Q<sup>2</sup> to Q<sup>0</sup> phosphate species. Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. Analysis of cells seeded onto the discs revealed that PBG with higher concentration of pyro- and orthophosphate content (61% Q<sup>1</sup> and 39% Q<sup>0</sup>) supported a 4.3-fold increase in adhered cells compared to glasses with metaphosphate connectivity (49% Q<sup>2</sup> and 51% Q<sup>1</sup>). This study highlights that tuning the composition of PBGs to possess pyro- and orthophosphate species only, enables the possibility to control cell adhesion performance. PBGs with superior cell adhesion profiles represent ideal candidates for biomedical applications, where cell recruitment and support for tissue ingrowth are of critical importance for orthopaedic interventions.https://www.mdpi.com/1422-0067/22/2/837biomaterialsstem cellsphosphate-based glassescell culturematerial degradation |
| spellingShingle | Nigel De Melo Lauren Murrell Md Towhidul Islam Jeremy J. Titman Laura Macri-Pellizzeri Ifty Ahmed Virginie Sottile Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses International Journal of Molecular Sciences biomaterials stem cells phosphate-based glasses cell culture material degradation |
| title | Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses |
| title_full | Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses |
| title_fullStr | Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses |
| title_full_unstemmed | Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses |
| title_short | Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses |
| title_sort | tailoring pyro and orthophosphate species to enhance stem cell adhesion to phosphate glasses |
| topic | biomaterials stem cells phosphate-based glasses cell culture material degradation |
| url | https://www.mdpi.com/1422-0067/22/2/837 |
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