Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota
Abstract Chondroitin sulfate (CS) has widely been used as a symptomatic slow-acting drug or a dietary supplement for the treatment and prevention of osteoarthritis. However, CS could not be absorbed after oral intake due to its polyanionic nature and large molecular weight. Gut microbiota has recent...
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| Format: | Article |
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BMC
2024-02-01
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| Series: | Microbiome |
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| Online Access: | https://doi.org/10.1186/s40168-024-01768-2 |
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| author | Yamin Wang Mingfeng Ma Wei Dai Qingsen Shang Guangli Yu |
| author_facet | Yamin Wang Mingfeng Ma Wei Dai Qingsen Shang Guangli Yu |
| author_sort | Yamin Wang |
| collection | DOAJ |
| description | Abstract Chondroitin sulfate (CS) has widely been used as a symptomatic slow-acting drug or a dietary supplement for the treatment and prevention of osteoarthritis. However, CS could not be absorbed after oral intake due to its polyanionic nature and large molecular weight. Gut microbiota has recently been proposed to play a pivotal role in the metabolism of drugs and nutrients. Nonetheless, how CS is degraded by the human gut microbiota has not been fully characterized. In the present study, we demonstrated that each human gut microbiota was characterized with a unique capability for CS degradation. Degradation and fermentation of CS by the human gut microbiota produced significant amounts of unsaturated CS oligosaccharides (CSOSs) and short-chain fatty acids. To uncover which microbes were responsible for CS degradation, we isolated a total of 586 bacterial strains with a potential CS-degrading capability from 23 human fecal samples. Bacteroides salyersiae was a potent species for CS degradation in the human gut microbiota and produced the highest amount of CSOSs as compared to other well-recognized CS-degraders, including Bacteroides finegoldii, Bacteroides thetaiotaomicron, Bacteroides xylanisolvens, and Bacteroides ovatus. Genomic analysis suggested that B. salyersiae was armed with multiple carbohydrate-active enzymes that could potentially degrade CS into CSOSs. By using a spent medium assay, we further demonstrated that the unsaturated tetrasaccharide (udp4) produced by the primary degrader B. salyersiae could serve as a “public goods” molecule for the growth of Bacteroides stercoris, a secondary CS-degrader that was proficient at fermenting CSOSs but not CS. Taken together, our study provides insights into the metabolism of CS by the human gut microbiota, which has promising implications for the development of medical and nutritional therapies for osteoarthritis. Video Abstract |
| first_indexed | 2024-03-07T14:49:35Z |
| format | Article |
| id | doaj.art-5f49503d34d74a4f8ab8e854e1138708 |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-03-07T14:49:35Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-5f49503d34d74a4f8ab8e854e11387082024-03-05T19:45:19ZengBMCMicrobiome2049-26182024-02-0112111210.1186/s40168-024-01768-2Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiotaYamin Wang0Mingfeng Ma1Wei Dai2Qingsen Shang3Guangli Yu4Key Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of ChinaKey Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of ChinaKey Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of ChinaKey Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of ChinaKey Laboratory of Marine Drugs of Ministry of Education, Shandong Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of ChinaAbstract Chondroitin sulfate (CS) has widely been used as a symptomatic slow-acting drug or a dietary supplement for the treatment and prevention of osteoarthritis. However, CS could not be absorbed after oral intake due to its polyanionic nature and large molecular weight. Gut microbiota has recently been proposed to play a pivotal role in the metabolism of drugs and nutrients. Nonetheless, how CS is degraded by the human gut microbiota has not been fully characterized. In the present study, we demonstrated that each human gut microbiota was characterized with a unique capability for CS degradation. Degradation and fermentation of CS by the human gut microbiota produced significant amounts of unsaturated CS oligosaccharides (CSOSs) and short-chain fatty acids. To uncover which microbes were responsible for CS degradation, we isolated a total of 586 bacterial strains with a potential CS-degrading capability from 23 human fecal samples. Bacteroides salyersiae was a potent species for CS degradation in the human gut microbiota and produced the highest amount of CSOSs as compared to other well-recognized CS-degraders, including Bacteroides finegoldii, Bacteroides thetaiotaomicron, Bacteroides xylanisolvens, and Bacteroides ovatus. Genomic analysis suggested that B. salyersiae was armed with multiple carbohydrate-active enzymes that could potentially degrade CS into CSOSs. By using a spent medium assay, we further demonstrated that the unsaturated tetrasaccharide (udp4) produced by the primary degrader B. salyersiae could serve as a “public goods” molecule for the growth of Bacteroides stercoris, a secondary CS-degrader that was proficient at fermenting CSOSs but not CS. Taken together, our study provides insights into the metabolism of CS by the human gut microbiota, which has promising implications for the development of medical and nutritional therapies for osteoarthritis. Video Abstracthttps://doi.org/10.1186/s40168-024-01768-2Bacteroides salyersiaeChondroitin sulfateGut microbiotaBacteroides stercorisGlycosaminoglycansDegradation |
| spellingShingle | Yamin Wang Mingfeng Ma Wei Dai Qingsen Shang Guangli Yu Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota Microbiome Bacteroides salyersiae Chondroitin sulfate Gut microbiota Bacteroides stercoris Glycosaminoglycans Degradation |
| title | Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota |
| title_full | Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota |
| title_fullStr | Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota |
| title_full_unstemmed | Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota |
| title_short | Bacteroides salyersiae is a potent chondroitin sulfate-degrading species in the human gut microbiota |
| title_sort | bacteroides salyersiae is a potent chondroitin sulfate degrading species in the human gut microbiota |
| topic | Bacteroides salyersiae Chondroitin sulfate Gut microbiota Bacteroides stercoris Glycosaminoglycans Degradation |
| url | https://doi.org/10.1186/s40168-024-01768-2 |
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