Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae)
Abstract Background Molecular taxonomy studies and barcoding projects can provide rapid means of detecting cryptic diversity. Nevertheless, the use of molecular data for species delimitation should be undertaken with caution. Especially the single-gene approaches are linked with certain pitfalls for...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | BMC Evolutionary Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12862-017-1032-x |
| _version_ | 1819115429584437248 |
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| author | Anja Palandačić Alexander Naseka David Ramler Harald Ahnelt |
| author_facet | Anja Palandačić Alexander Naseka David Ramler Harald Ahnelt |
| author_sort | Anja Palandačić |
| collection | DOAJ |
| description | Abstract Background Molecular taxonomy studies and barcoding projects can provide rapid means of detecting cryptic diversity. Nevertheless, the use of molecular data for species delimitation should be undertaken with caution. Especially the single-gene approaches are linked with certain pitfalls for taxonomical inference. In the present study, recent and historical species descriptions based upon morphology were used as primary species hypotheses, which were then evaluated with molecular data (including in type and historical museum material) to form secondary species hypotheses. As an example of cryptic diversity and taxonomic controversy, the European Phoxinus phoxinus species complex was used. Results The results of the revision showed that of the fourteen primary species hypotheses, three were rejected, namely P. ketmaieri, P. likai, and P. apollonicus. For three species (P. strandjae, P. strymonicus, P. morella), further investigation with increased data sampling was suggested, while two primary hypotheses, P. bigerri and P. colchicus, were supported as secondary species hypotheses. Finally, six of the primary species hypotheses (P. phoxinus, P. lumaireul, P. karsticus, P. septimanae, P. marsilii and P. csikii) were well supported by mitochondrial but only limitedly corroborated by nuclear data analysis. Conclusion The approach has proven useful for revision of species complexes, and the study can serve as an overview of the Phoxinus genus in Europe, as well as a solid basis for further work. |
| first_indexed | 2024-12-22T05:01:03Z |
| format | Article |
| id | doaj.art-5f4b511612824dd5834b6accb98d55f2 |
| institution | Directory Open Access Journal |
| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-12-22T05:01:03Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Evolutionary Biology |
| spelling | doaj.art-5f4b511612824dd5834b6accb98d55f22022-12-21T18:38:15ZengBMCBMC Evolutionary Biology1471-21482017-08-0117111710.1186/s12862-017-1032-xContrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae)Anja Palandačić0Alexander Naseka1David Ramler2Harald Ahnelt3First Zoological Department, Vienna Museum of Natural HistoryFirst Zoological Department, Vienna Museum of Natural HistoryDepartment of Limnology and Bio-Oceanography, University of ViennaFirst Zoological Department, Vienna Museum of Natural HistoryAbstract Background Molecular taxonomy studies and barcoding projects can provide rapid means of detecting cryptic diversity. Nevertheless, the use of molecular data for species delimitation should be undertaken with caution. Especially the single-gene approaches are linked with certain pitfalls for taxonomical inference. In the present study, recent and historical species descriptions based upon morphology were used as primary species hypotheses, which were then evaluated with molecular data (including in type and historical museum material) to form secondary species hypotheses. As an example of cryptic diversity and taxonomic controversy, the European Phoxinus phoxinus species complex was used. Results The results of the revision showed that of the fourteen primary species hypotheses, three were rejected, namely P. ketmaieri, P. likai, and P. apollonicus. For three species (P. strandjae, P. strymonicus, P. morella), further investigation with increased data sampling was suggested, while two primary hypotheses, P. bigerri and P. colchicus, were supported as secondary species hypotheses. Finally, six of the primary species hypotheses (P. phoxinus, P. lumaireul, P. karsticus, P. septimanae, P. marsilii and P. csikii) were well supported by mitochondrial but only limitedly corroborated by nuclear data analysis. Conclusion The approach has proven useful for revision of species complexes, and the study can serve as an overview of the Phoxinus genus in Europe, as well as a solid basis for further work.http://link.springer.com/article/10.1186/s12862-017-1032-xCryptic diversitySpecies delimitationMolecular taxonomyPhoxinus (Cyprinidae) |
| spellingShingle | Anja Palandačić Alexander Naseka David Ramler Harald Ahnelt Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) BMC Evolutionary Biology Cryptic diversity Species delimitation Molecular taxonomy Phoxinus (Cyprinidae) |
| title | Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) |
| title_full | Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) |
| title_fullStr | Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) |
| title_full_unstemmed | Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) |
| title_short | Contrasting morphology with molecular data: an approach to revision of species complexes based on the example of European Phoxinus (Cyprinidae) |
| title_sort | contrasting morphology with molecular data an approach to revision of species complexes based on the example of european phoxinus cyprinidae |
| topic | Cryptic diversity Species delimitation Molecular taxonomy Phoxinus (Cyprinidae) |
| url | http://link.springer.com/article/10.1186/s12862-017-1032-x |
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