Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms.
BACKGROUND:Currently, clinical examination, ultrasound scanning (with or without fine needle aspiration cytology), preoperative CT-scan and MRI are available for the differential diagnosis of parotid gland swelling. A preliminary non-invasive salivary diagnostic tool may be helpful in the clinical d...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4636154?pdf=render |
| _version_ | 1818337267971784704 |
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| author | Johannes H Matse Janice Yoshizawa Xiaoyan Wang David Elashoff Jan G M Bolscher Enno C I Veerman C René Leemans D Michiel Pegtel David T W Wong Elisabeth Bloemena |
| author_facet | Johannes H Matse Janice Yoshizawa Xiaoyan Wang David Elashoff Jan G M Bolscher Enno C I Veerman C René Leemans D Michiel Pegtel David T W Wong Elisabeth Bloemena |
| author_sort | Johannes H Matse |
| collection | DOAJ |
| description | BACKGROUND:Currently, clinical examination, ultrasound scanning (with or without fine needle aspiration cytology), preoperative CT-scan and MRI are available for the differential diagnosis of parotid gland swelling. A preliminary non-invasive salivary diagnostic tool may be helpful in the clinical decision making process. Altered salivary micro-RNA (miRNA) expression levels have been observed in saliva from patients with various cancers. Therefore, we investigated miRNA expression levels in saliva samples from patients with a parotid gland neoplasm using Human miRNA cards in comparison to controls. RESULTS:In the discovery phase, eight miRNAs were identified having different expression levels in patients compared to controls. In the validation phase, the differences in miRNA expression levels between patients and controls were confirmed for seven out of eight discovered miRNAs (p < 0.001). A combination of two miRNAs yielded a receiver-operator-characteristics curve with an AUC of 0.94 (95% CI: 0.87-1.00; sensitivity 91%; specificity 86%). Validation of discovered miRNAs in segregated collected parotid saliva revealed that expression of these miRNAs differ between whole saliva and parotid saliva. CONCLUSIONS:A two miRNA combination can predict the presence of a parotid gland neoplasm. Furthermore, this study suggested that the identified, patient-specific, salivary miRNAs were not derived from the parotid gland itself. |
| first_indexed | 2024-12-13T14:52:31Z |
| format | Article |
| id | doaj.art-5f4b85317a5b4ce28e26856471686bd2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:52:31Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5f4b85317a5b4ce28e26856471686bd22022-12-21T23:41:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014226410.1371/journal.pone.0142264Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms.Johannes H MatseJanice YoshizawaXiaoyan WangDavid ElashoffJan G M BolscherEnno C I VeermanC René LeemansD Michiel PegtelDavid T W WongElisabeth BloemenaBACKGROUND:Currently, clinical examination, ultrasound scanning (with or without fine needle aspiration cytology), preoperative CT-scan and MRI are available for the differential diagnosis of parotid gland swelling. A preliminary non-invasive salivary diagnostic tool may be helpful in the clinical decision making process. Altered salivary micro-RNA (miRNA) expression levels have been observed in saliva from patients with various cancers. Therefore, we investigated miRNA expression levels in saliva samples from patients with a parotid gland neoplasm using Human miRNA cards in comparison to controls. RESULTS:In the discovery phase, eight miRNAs were identified having different expression levels in patients compared to controls. In the validation phase, the differences in miRNA expression levels between patients and controls were confirmed for seven out of eight discovered miRNAs (p < 0.001). A combination of two miRNAs yielded a receiver-operator-characteristics curve with an AUC of 0.94 (95% CI: 0.87-1.00; sensitivity 91%; specificity 86%). Validation of discovered miRNAs in segregated collected parotid saliva revealed that expression of these miRNAs differ between whole saliva and parotid saliva. CONCLUSIONS:A two miRNA combination can predict the presence of a parotid gland neoplasm. Furthermore, this study suggested that the identified, patient-specific, salivary miRNAs were not derived from the parotid gland itself.http://europepmc.org/articles/PMC4636154?pdf=render |
| spellingShingle | Johannes H Matse Janice Yoshizawa Xiaoyan Wang David Elashoff Jan G M Bolscher Enno C I Veerman C René Leemans D Michiel Pegtel David T W Wong Elisabeth Bloemena Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. PLoS ONE |
| title | Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. |
| title_full | Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. |
| title_fullStr | Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. |
| title_full_unstemmed | Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. |
| title_short | Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. |
| title_sort | human salivary micro rna in patients with parotid salivary gland neoplasms |
| url | http://europepmc.org/articles/PMC4636154?pdf=render |
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