Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis
Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/11/302 |
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| author | Rossana Scutari Lorenzo Piermatteo Matteo Ciancio Manuelli Marco Iannetta Romina Salpini Ada Bertoli Claudia Alteri Patrizia Saccomandi Maria Concetta Bellocchi Vincenzo Malagnino Elisabetta Teti Daniele Sforza Leandro Siragusa Michele Grande Loredana Sarmati Valentina Svicher Massimo Andreoni Francesca Ceccherini-Silberstein |
| author_facet | Rossana Scutari Lorenzo Piermatteo Matteo Ciancio Manuelli Marco Iannetta Romina Salpini Ada Bertoli Claudia Alteri Patrizia Saccomandi Maria Concetta Bellocchi Vincenzo Malagnino Elisabetta Teti Daniele Sforza Leandro Siragusa Michele Grande Loredana Sarmati Valentina Svicher Massimo Andreoni Francesca Ceccherini-Silberstein |
| author_sort | Rossana Scutari |
| collection | DOAJ |
| description | Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations. |
| first_indexed | 2024-03-10T14:38:53Z |
| format | Article |
| id | doaj.art-5f528399d0534166804889e60c822944 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T14:38:53Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-5f528399d0534166804889e60c8229442023-11-20T21:56:46ZengMDPI AGLife2075-17292020-11-01101130210.3390/life10110302Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute CholecystitisRossana Scutari0Lorenzo Piermatteo1Matteo Ciancio Manuelli2Marco Iannetta3Romina Salpini4Ada Bertoli5Claudia Alteri6Patrizia Saccomandi7Maria Concetta Bellocchi8Vincenzo Malagnino9Elisabetta Teti10Daniele Sforza11Leandro Siragusa12Michele Grande13Loredana Sarmati14Valentina Svicher15Massimo Andreoni16Francesca Ceccherini-Silberstein17Department of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalySurgical Emergency Unit, Emergency Department, Polyclinic Tor Vergata Foundation, 00133 Rome, ItalyDepartment of System Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Oncology and Hemato-oncology, University of Milan, 20122 Milan, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of System Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of System Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalySurgical Emergency Unit, Emergency Department, Polyclinic Tor Vergata Foundation, 00133 Rome, ItalyDepartment of Surgical Sciences, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Surgical Sciences, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of System Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of System Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyOur study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations.https://www.mdpi.com/2075-1729/10/11/302SARS-CoV-2RNA quantificationdroplet digital PCRRT-PCRbody compartment |
| spellingShingle | Rossana Scutari Lorenzo Piermatteo Matteo Ciancio Manuelli Marco Iannetta Romina Salpini Ada Bertoli Claudia Alteri Patrizia Saccomandi Maria Concetta Bellocchi Vincenzo Malagnino Elisabetta Teti Daniele Sforza Leandro Siragusa Michele Grande Loredana Sarmati Valentina Svicher Massimo Andreoni Francesca Ceccherini-Silberstein Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis Life SARS-CoV-2 RNA quantification droplet digital PCR RT-PCR body compartment |
| title | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
| title_full | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
| title_fullStr | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
| title_full_unstemmed | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
| title_short | Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute Cholecystitis |
| title_sort | long term sars cov 2 infection associated with viral dissemination in different body fluids including bile in two patients with acute cholecystitis |
| topic | SARS-CoV-2 RNA quantification droplet digital PCR RT-PCR body compartment |
| url | https://www.mdpi.com/2075-1729/10/11/302 |
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