Nanofibrous materials affect the reaction of cytotoxicity assays
Abstract Nanofibrous materials are widely investigated as a replacement for the extracellular matrix, the 3D foundation for cells in all tissues. However, as with every medical material, nanofibers too must pass all safety evaluations like in vitro cytotoxicity assays or in vivo animal tests. Our li...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13002-w |
| _version_ | 1818532565165801472 |
|---|---|
| author | Rafał Podgórski Michał Wojasiński Tomasz Ciach |
| author_facet | Rafał Podgórski Michał Wojasiński Tomasz Ciach |
| author_sort | Rafał Podgórski |
| collection | DOAJ |
| description | Abstract Nanofibrous materials are widely investigated as a replacement for the extracellular matrix, the 3D foundation for cells in all tissues. However, as with every medical material, nanofibers too must pass all safety evaluations like in vitro cytotoxicity assays or in vivo animal tests. Our literature research showed that differences in results of widely used cytotoxicity assays applied to evaluate nanofibrous materials are poorly understood. To better explore this issue, we prepared three nanofibrous materials with similar physical properties made of poly-L-lactic acid, polyurethane, and polycaprolactone. We tested five metabolic cytotoxicity assays (MTT, XTT, CCK-8, alamarBlue, PrestoBlue) and obtained different viability results for the same nanofibrous materials. Further, the study revealed that nanofibrous materials affect the reaction of cytotoxicity assays. Considering the results of both described experiments, it is evident that validating all available cytotoxicity assays for nanofibrous materials and possibly other highly porous materials should be carefully planned and verified using an additional analytical tool, like scanning electron microscopy or, more preferably, confocal microscopy. |
| first_indexed | 2024-12-11T17:47:11Z |
| format | Article |
| id | doaj.art-5f535d26dcd84f8a98e404bd4a422b7f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T17:47:11Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5f535d26dcd84f8a98e404bd4a422b7f2022-12-22T00:56:19ZengNature PortfolioScientific Reports2045-23222022-05-0112111210.1038/s41598-022-13002-wNanofibrous materials affect the reaction of cytotoxicity assaysRafał Podgórski0Michał Wojasiński1Tomasz Ciach2Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Laboratory of Biomedical Engineering, Warsaw University of TechnologyFaculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Laboratory of Biomedical Engineering, Warsaw University of TechnologyFaculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Laboratory of Biomedical Engineering, Warsaw University of TechnologyAbstract Nanofibrous materials are widely investigated as a replacement for the extracellular matrix, the 3D foundation for cells in all tissues. However, as with every medical material, nanofibers too must pass all safety evaluations like in vitro cytotoxicity assays or in vivo animal tests. Our literature research showed that differences in results of widely used cytotoxicity assays applied to evaluate nanofibrous materials are poorly understood. To better explore this issue, we prepared three nanofibrous materials with similar physical properties made of poly-L-lactic acid, polyurethane, and polycaprolactone. We tested five metabolic cytotoxicity assays (MTT, XTT, CCK-8, alamarBlue, PrestoBlue) and obtained different viability results for the same nanofibrous materials. Further, the study revealed that nanofibrous materials affect the reaction of cytotoxicity assays. Considering the results of both described experiments, it is evident that validating all available cytotoxicity assays for nanofibrous materials and possibly other highly porous materials should be carefully planned and verified using an additional analytical tool, like scanning electron microscopy or, more preferably, confocal microscopy.https://doi.org/10.1038/s41598-022-13002-w |
| spellingShingle | Rafał Podgórski Michał Wojasiński Tomasz Ciach Nanofibrous materials affect the reaction of cytotoxicity assays Scientific Reports |
| title | Nanofibrous materials affect the reaction of cytotoxicity assays |
| title_full | Nanofibrous materials affect the reaction of cytotoxicity assays |
| title_fullStr | Nanofibrous materials affect the reaction of cytotoxicity assays |
| title_full_unstemmed | Nanofibrous materials affect the reaction of cytotoxicity assays |
| title_short | Nanofibrous materials affect the reaction of cytotoxicity assays |
| title_sort | nanofibrous materials affect the reaction of cytotoxicity assays |
| url | https://doi.org/10.1038/s41598-022-13002-w |
| work_keys_str_mv | AT rafałpodgorski nanofibrousmaterialsaffectthereactionofcytotoxicityassays AT michałwojasinski nanofibrousmaterialsaffectthereactionofcytotoxicityassays AT tomaszciach nanofibrousmaterialsaffectthereactionofcytotoxicityassays |