The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy
Vasorin (VASN), a transmembrane protein heavily expressed in endothelial cells, has garnered recent interest due to its key role in vascular development and pathology. The oligomeric state of VASN is a crucial piece of knowledge given that receptor clustering is a frequent regulatory mechanism in do...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/7/4115 |
| _version_ | 1797212431279194112 |
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| author | Junyi Liang Adam W. Smith |
| author_facet | Junyi Liang Adam W. Smith |
| author_sort | Junyi Liang |
| collection | DOAJ |
| description | Vasorin (VASN), a transmembrane protein heavily expressed in endothelial cells, has garnered recent interest due to its key role in vascular development and pathology. The oligomeric state of VASN is a crucial piece of knowledge given that receptor clustering is a frequent regulatory mechanism in downstream signaling activation and amplification. However, documentation of VASN oligomerization is currently absent. In this brief report, we describe the measurement of VASN oligomerization in its native membranous environment, leveraging a class of fluorescence fluctuation spectroscopy. Our investigation revealed that the majority of VASN resides in a monomeric state, while a minority of VASN forms homodimers in the cellular membrane. This result raises the intriguing possibility that ligand-independent clustering of VASN may play a role in transforming growth factor signaling. |
| first_indexed | 2024-04-24T10:42:16Z |
| format | Article |
| id | doaj.art-5f5474f8a84b4a93bf012e8b9a42db3d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T10:42:16Z |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f5474f8a84b4a93bf012e8b9a42db3d2024-04-12T13:20:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257411510.3390/ijms25074115The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation SpectroscopyJunyi Liang0Adam W. Smith1Department of Chemistry, University of Akron, Akron, OH 44325, USADepartment of Chemistry, University of Akron, Akron, OH 44325, USAVasorin (VASN), a transmembrane protein heavily expressed in endothelial cells, has garnered recent interest due to its key role in vascular development and pathology. The oligomeric state of VASN is a crucial piece of knowledge given that receptor clustering is a frequent regulatory mechanism in downstream signaling activation and amplification. However, documentation of VASN oligomerization is currently absent. In this brief report, we describe the measurement of VASN oligomerization in its native membranous environment, leveraging a class of fluorescence fluctuation spectroscopy. Our investigation revealed that the majority of VASN resides in a monomeric state, while a minority of VASN forms homodimers in the cellular membrane. This result raises the intriguing possibility that ligand-independent clustering of VASN may play a role in transforming growth factor signaling.https://www.mdpi.com/1422-0067/25/7/4115vasorin (VASN)fluorescent proteinsfluorescence fluctuation spectroscopymembrane protein multimerization |
| spellingShingle | Junyi Liang Adam W. Smith The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy International Journal of Molecular Sciences vasorin (VASN) fluorescent proteins fluorescence fluctuation spectroscopy membrane protein multimerization |
| title | The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy |
| title_full | The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy |
| title_fullStr | The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy |
| title_full_unstemmed | The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy |
| title_short | The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy |
| title_sort | oligomeric state of vasorin in the plasma membrane measured non invasively by quantitative fluorescence fluctuation spectroscopy |
| topic | vasorin (VASN) fluorescent proteins fluorescence fluctuation spectroscopy membrane protein multimerization |
| url | https://www.mdpi.com/1422-0067/25/7/4115 |
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