Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips
In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifica...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/10/2/129 |
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| author | Tatyana O. Pleshakova Anna L. Kaysheva Ivan D. Shumov Vadim S. Ziborov Jana M. Bayzyanova Vladimir A. Konev Vasiliy F. Uchaikin Alexander I. Archakov Yuri D. Ivanov |
| author_facet | Tatyana O. Pleshakova Anna L. Kaysheva Ivan D. Shumov Vadim S. Ziborov Jana M. Bayzyanova Vladimir A. Konev Vasiliy F. Uchaikin Alexander I. Archakov Yuri D. Ivanov |
| author_sort | Tatyana O. Pleshakova |
| collection | DOAJ |
| description | In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifically captured onto the chip surface from 1 mL of test solution containing 10 µL of serum collected from a hepatitis C patient. The registration of aptamer/antigen complexes on the chip surface was performed by AFM. The aptamers used in the present study were initially developed for therapeutic purposes; herein, these aptamers have been successfully utilized as probe molecules for HCVcoreAg detection in the presence of a complex protein matrix (human serum). The results obtained herein can be used for the development of detection systems that employ affine enrichment for protein detection. |
| first_indexed | 2024-12-23T14:30:14Z |
| format | Article |
| id | doaj.art-5f553ab4d5054fd58f51cf425d1b344d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-23T14:30:14Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-5f553ab4d5054fd58f51cf425d1b344d2022-12-21T17:43:32ZengMDPI AGMicromachines2072-666X2019-02-0110212910.3390/mi10020129mi10020129Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM ChipsTatyana O. Pleshakova0Anna L. Kaysheva1Ivan D. Shumov2Vadim S. Ziborov3Jana M. Bayzyanova4Vladimir A. Konev5Vasiliy F. Uchaikin6Alexander I. Archakov7Yuri D. Ivanov8Institute of Biomedical Chemistry, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Moscow 119121, RussiaPirogov Russian National Research Medical University (RNRMU), Moscow 117997, RussiaPirogov Russian National Research Medical University (RNRMU), Moscow 117997, RussiaPirogov Russian National Research Medical University (RNRMU), Moscow 117997, RussiaInstitute of Biomedical Chemistry, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Moscow 119121, RussiaIn the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifically captured onto the chip surface from 1 mL of test solution containing 10 µL of serum collected from a hepatitis C patient. The registration of aptamer/antigen complexes on the chip surface was performed by AFM. The aptamers used in the present study were initially developed for therapeutic purposes; herein, these aptamers have been successfully utilized as probe molecules for HCVcoreAg detection in the presence of a complex protein matrix (human serum). The results obtained herein can be used for the development of detection systems that employ affine enrichment for protein detection.https://www.mdpi.com/2072-666X/10/2/129atomic force microscopyaptamerprotein detectionviral hepatitismass spectrometry |
| spellingShingle | Tatyana O. Pleshakova Anna L. Kaysheva Ivan D. Shumov Vadim S. Ziborov Jana M. Bayzyanova Vladimir A. Konev Vasiliy F. Uchaikin Alexander I. Archakov Yuri D. Ivanov Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips Micromachines atomic force microscopy aptamer protein detection viral hepatitis mass spectrometry |
| title | Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips |
| title_full | Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips |
| title_fullStr | Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips |
| title_full_unstemmed | Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips |
| title_short | Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips |
| title_sort | detection of hepatitis c virus core protein in serum using aptamer functionalized afm chips |
| topic | atomic force microscopy aptamer protein detection viral hepatitis mass spectrometry |
| url | https://www.mdpi.com/2072-666X/10/2/129 |
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