Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips

Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips

In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifica...

Full description

Bibliographic Details
Main Authors:	Tatyana O. Pleshakova, Anna L. Kaysheva, Ivan D. Shumov, Vadim S. Ziborov, Jana M. Bayzyanova, Vladimir A. Konev, Vasiliy F. Uchaikin, Alexander I. Archakov, Yuri D. Ivanov
Format:	Article
Language:	English
Published:	MDPI AG 2019-02-01
Series:	Micromachines
Subjects:	atomic force microscopy aptamer protein detection viral hepatitis mass spectrometry
Online Access:	https://www.mdpi.com/2072-666X/10/2/129

Similar Items

Selection of Aptamers for Use as Molecular Probes in AFM Detection of Proteins
by: Maria O. Ershova, et al.
Published: (2023-12-01)

Detection of Circulating Serum microRNA/Protein Complexes in ASD Using Functionalized Chips for an Atomic Force Microscope
by: Anna L. Kaysheva, et al.
Published: (2021-10-01)

Mass Spectrometric Identification of BSA Covalently Captured onto a Chip for Atomic Force Microscopy
by: Arina I. Gordeeva, et al.
Published: (2023-05-01)

The Study of Performance of a Nanoribbon Biosensor, Sensitized with Aptamers and Antibodies, upon Detection of Core Antigen of Hepatitis C Virus
by: Yuri D. Ivanov, et al.
Published: (2023-10-01)

AFM Study of the Influence of Glycerol Flow on Horseradish Peroxidase near the in/out Linear Sections of a Coil
by: Yuri D. Ivanov, et al.
Published: (2021-02-01)

Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker
by: Anastasia A. Valueva, et al.
Published: (2020-05-01)

Aptamer-Sensitized Nanoribbon Biosensor for Ovarian Cancer Marker Detection in Plasma
by: Yuri D. Ivanov, et al.
Published: (2021-08-01)

Mass Spectrometric Identification of Proteins Enhanced by the Atomic Force Microscopy Immobilization Surface
by: Anna L. Kaysheva, et al.
Published: (2021-01-01)

MS Identification of Blood Plasma Proteins Concentrated on a Photocrosslinker-Modified Surface
by: Arina I. Gordeeva, et al.
Published: (2023-12-01)

A review on the therapeutic applications of aptamers and aptamer-conjugated nanoparticles in cancer, inflammatory and viral diseases
by: Suliman Khan, et al.
Published: (2022-02-01)

Selection and Characterization of DNA Aptamers for Constructing Aptamer-AuNPs Colorimetric Method for Detection of AFM1
by: Ruobing Liu, et al.
Published: (2022-06-01)

Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow
by: Yuri D. Ivanov, et al.
Published: (2020-06-01)

Aptamers: Biomedical Interest and Applications
by: Cristina Romero-López, et al.
Published: (2017-03-01)

Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents
by: Prabir Kumar Kulabhusan, et al.
Published: (2020-07-01)

Novel Photochrome Aptamer Switch Assay (PHASA) for Adaptive Binding to Aptamers
by: Papper, Vladislav, et al.
Published: (2017)

Aptamer Applications in Emerging Viral Diseases
by: Arne Krüger, et al.
Published: (2021-06-01)

Selection and Characterization of Single-Stranded DNA Aptamers of Diagnostic Potential against the Whole Zika Virus
by: Liliane Monteiro de Morais, et al.
Published: (2022-08-01)

AFM Investigation of the Influence of Steam Flow through a Conical Coil Heat Exchanger on Enzyme Properties
by: Yuri D. Ivanov, et al.
Published: (2022-11-01)

An Aptamer for Broad Cancer Targeting and Therapy
by: Bethany Powell Gray, et al.
Published: (2020-10-01)

Aptamer-Mediated Antiviral Approaches for SARS-CoV-2
by: Kei Xian Tan, et al.
Published: (2022-11-01)

Influence of Chip Materials on Charge Generation in Flowing Solution in Nanobiosensors
by: Yuri D. Ivanov, et al.
Published: (2019-02-01)

Optimization of Short RNA Aptamers for TNBC Cell Targeting
by: Simona Camorani, et al.
Published: (2022-03-01)

Radiolabeling of Nucleic Acid Aptamers for Highly Sensitive Disease-Specific Molecular Imaging
by: Leila Hassanzadeh, et al.
Published: (2018-10-01)

Aptamers and Cancer
by: Nasrin Yazdanpanahi, et al.
Published: (2016-04-01)

CD28 Aptamers as Powerful Immune Response Modulators
by: Fernando Pastor, et al.
Published: (2013-01-01)

Highly Selective Aptamer‐Molecularly Imprinted Polymer Hybrids for Recognition of SARS‐CoV‐2 Spike Protein Variants
by: Mark V. Sullivan, et al.
Published: (2023-06-01)

Thermal Stability of siRNA Modulates Aptamer- conjugated siRNA Inhibition
by: Alexey Berezhnoy, et al.
Published: (2012-01-01)

Improvement of Aptamer Affinity by Dimerization
by: Kazunori Ikebukuro, et al.
Published: (2008-02-01)

Two Examples of RNA Aptamers with Antiviral Activity. Are Aptamers the Wished Antiviral Drugs?
by: Alfredo Berzal-Herranz, et al.
Published: (2020-07-01)

Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels
by: Ya Di, et al.
Published: (2020-10-01)

Competitive aptamer switch for modulating ligand binding affinity
by: Derek Puyat, et al.
Published: (2023-09-01)

AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase
by: Yuri D. Ivanov, et al.
Published: (2021-01-01)

Aptamer-Based Imaging of Polyisoprenoids in the Malaria Parasite
by: Flavia M. Zimbres, et al.
Published: (2023-12-01)

G-Quadruplex Aptamer-Ligand Characterization
by: David Moreira, et al.
Published: (2022-10-01)

Methods for Improving Aptamer Binding Affinity
by: Hijiri Hasegawa, et al.
Published: (2016-03-01)

Two Interconvertible Folds Modulate the Activity of a DNA Aptamer Against Transferrin Receptor
by: David Porciani, et al.
Published: (2014-01-01)

Aptamers and cancer
by: Nasrin Yazdanpanahi, et al.
Published: (2016-04-01)

Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase
by: Alice Sosic, et al.
Published: (2011-10-01)

AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
by: Yuri D. Ivanov, et al.
Published: (2021-05-01)

Design and Prediction of Aptamers Assisted by In Silico Methods
by: Su Jin Lee, et al.
Published: (2023-01-01)