A new perfusion culture method with a self-organized capillary network.
A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0240552 |
| _version_ | 1818728477338107904 |
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| author | Kei Sugihara Yoshimi Yamaguchi Shiori Usui Yuji Nashimoto Sanshiro Hanada Etsuko Kiyokawa Akiyoshi Uemura Ryuji Yokokawa Koichi Nishiyama Takashi Miura |
| author_facet | Kei Sugihara Yoshimi Yamaguchi Shiori Usui Yuji Nashimoto Sanshiro Hanada Etsuko Kiyokawa Akiyoshi Uemura Ryuji Yokokawa Koichi Nishiyama Takashi Miura |
| author_sort | Kei Sugihara |
| collection | DOAJ |
| description | A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow. |
| first_indexed | 2024-12-17T22:30:37Z |
| format | Article |
| id | doaj.art-5f58b62a96c64c5a9dad79dd41e59ad2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:30:37Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5f58b62a96c64c5a9dad79dd41e59ad22022-12-21T21:30:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e024055210.1371/journal.pone.0240552A new perfusion culture method with a self-organized capillary network.Kei SugiharaYoshimi YamaguchiShiori UsuiYuji NashimotoSanshiro HanadaEtsuko KiyokawaAkiyoshi UemuraRyuji YokokawaKoichi NishiyamaTakashi MiuraA lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow.https://doi.org/10.1371/journal.pone.0240552 |
| spellingShingle | Kei Sugihara Yoshimi Yamaguchi Shiori Usui Yuji Nashimoto Sanshiro Hanada Etsuko Kiyokawa Akiyoshi Uemura Ryuji Yokokawa Koichi Nishiyama Takashi Miura A new perfusion culture method with a self-organized capillary network. PLoS ONE |
| title | A new perfusion culture method with a self-organized capillary network. |
| title_full | A new perfusion culture method with a self-organized capillary network. |
| title_fullStr | A new perfusion culture method with a self-organized capillary network. |
| title_full_unstemmed | A new perfusion culture method with a self-organized capillary network. |
| title_short | A new perfusion culture method with a self-organized capillary network. |
| title_sort | new perfusion culture method with a self organized capillary network |
| url | https://doi.org/10.1371/journal.pone.0240552 |
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