Packaging of DNA Integrated with Metal Nanoparticles in Solution
The transformation of high-molecular DNA from a random swollen coil in a solution to a discrete nanosized particle with the ordered packaging of a rigid and highly charged double-stranded molecule is one of the amazing phenomena of polymer physics. DNA condensation is a well-known phenomenon in biol...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Entropy |
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| Online Access: | https://www.mdpi.com/1099-4300/25/7/1052 |
| _version_ | 1797589369502040064 |
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| author | Nina Kasyanenko Andrei Baryshev Daria Artamonova Petr Sokolov |
| author_facet | Nina Kasyanenko Andrei Baryshev Daria Artamonova Petr Sokolov |
| author_sort | Nina Kasyanenko |
| collection | DOAJ |
| description | The transformation of high-molecular DNA from a random swollen coil in a solution to a discrete nanosized particle with the ordered packaging of a rigid and highly charged double-stranded molecule is one of the amazing phenomena of polymer physics. DNA condensation is a well-known phenomenon in biological systems, yet its molecular mechanism is not clear. Understanding the processes occurring in vivo is necessary for the usage of DNA in the fabrication of new biologically significant nanostructures. Entropy plays a very important role in DNA condensation. DNA conjugates with metal nanoparticles are useful in various fields of nanotechnology. In particular, they can serve as a basis for creating multicomponent nanoplatforms for theranostics. DNA must be in a compact state in such constructions. In this paper, we tested the methods of DNA integration with silver, gold and palladium nanoparticles and analyzed the properties of DNA conjugates with metal nanoparticles using the methods of atomic force microscopy, spectroscopy, viscometry and dynamic light scattering. DNA size, stability and rigidity (persistence length), as well as plasmon resonance peaks in the absorption spectra of systems were studied. The methods for DNA condensation with metal nanoparticles were analyzed. |
| first_indexed | 2024-03-11T01:05:38Z |
| format | Article |
| id | doaj.art-5f5b1369c7fb4d39a519a373a0353e74 |
| institution | Directory Open Access Journal |
| issn | 1099-4300 |
| language | English |
| last_indexed | 2024-03-11T01:05:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Entropy |
| spelling | doaj.art-5f5b1369c7fb4d39a519a373a0353e742023-11-18T19:14:03ZengMDPI AGEntropy1099-43002023-07-01257105210.3390/e25071052Packaging of DNA Integrated with Metal Nanoparticles in SolutionNina Kasyanenko0Andrei Baryshev1Daria Artamonova2Petr Sokolov3Faculty of Physics, Saint Petersburg State University, Saint Petersburg 199034, RussiaFaculty of Physics, Saint Petersburg State University, Saint Petersburg 199034, RussiaFaculty of Physics, Saint Petersburg State University, Saint Petersburg 199034, RussiaFaculty of Physics, Saint Petersburg State University, Saint Petersburg 199034, RussiaThe transformation of high-molecular DNA from a random swollen coil in a solution to a discrete nanosized particle with the ordered packaging of a rigid and highly charged double-stranded molecule is one of the amazing phenomena of polymer physics. DNA condensation is a well-known phenomenon in biological systems, yet its molecular mechanism is not clear. Understanding the processes occurring in vivo is necessary for the usage of DNA in the fabrication of new biologically significant nanostructures. Entropy plays a very important role in DNA condensation. DNA conjugates with metal nanoparticles are useful in various fields of nanotechnology. In particular, they can serve as a basis for creating multicomponent nanoplatforms for theranostics. DNA must be in a compact state in such constructions. In this paper, we tested the methods of DNA integration with silver, gold and palladium nanoparticles and analyzed the properties of DNA conjugates with metal nanoparticles using the methods of atomic force microscopy, spectroscopy, viscometry and dynamic light scattering. DNA size, stability and rigidity (persistence length), as well as plasmon resonance peaks in the absorption spectra of systems were studied. The methods for DNA condensation with metal nanoparticles were analyzed.https://www.mdpi.com/1099-4300/25/7/1052DNA conjugates with metal nanoparticlessilver nanoparticlesgold nanoparticlesplatinum nanoparticlesDNA rigidityDNA condensation |
| spellingShingle | Nina Kasyanenko Andrei Baryshev Daria Artamonova Petr Sokolov Packaging of DNA Integrated with Metal Nanoparticles in Solution Entropy DNA conjugates with metal nanoparticles silver nanoparticles gold nanoparticles platinum nanoparticles DNA rigidity DNA condensation |
| title | Packaging of DNA Integrated with Metal Nanoparticles in Solution |
| title_full | Packaging of DNA Integrated with Metal Nanoparticles in Solution |
| title_fullStr | Packaging of DNA Integrated with Metal Nanoparticles in Solution |
| title_full_unstemmed | Packaging of DNA Integrated with Metal Nanoparticles in Solution |
| title_short | Packaging of DNA Integrated with Metal Nanoparticles in Solution |
| title_sort | packaging of dna integrated with metal nanoparticles in solution |
| topic | DNA conjugates with metal nanoparticles silver nanoparticles gold nanoparticles platinum nanoparticles DNA rigidity DNA condensation |
| url | https://www.mdpi.com/1099-4300/25/7/1052 |
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