In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes
Carrots are a multi-purpose vegetable; hence, they have become a very popular consumers’ choice, also thanks to their nutritional value with health benefits. Several varieties and landraces exist of carrot (<i>Daucus carota</i> L. ssp. <i>sativus</i>). In Apulia (Italy), for...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/12/1150 |
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| author | Claudia Ruta Angela Campanelli Giuseppe De Mastro Federica Blando |
| author_facet | Claudia Ruta Angela Campanelli Giuseppe De Mastro Federica Blando |
| author_sort | Claudia Ruta |
| collection | DOAJ |
| description | Carrots are a multi-purpose vegetable; hence, they have become a very popular consumers’ choice, also thanks to their nutritional value with health benefits. Several varieties and landraces exist of carrot (<i>Daucus carota</i> L. ssp. <i>sativus</i>). In Apulia (Italy), for example, a multicolored carrot landrace is present, the Polignano carrot (PC), threatened by the risk of genetic erosion. With the purpose of verifying the possibility of using in vitro culture for ex situ conservation purposes of PC, a rapid and reliable protocol for micropropagation and a highly efficient somatic embryogenesis protocol have been set up. For micropropagation studies, axillary buds from taproot were used as initial explants. Supplementation on basal medium (BM, a Murashige–Skoog modified medium) with low cytokinine (6-benzyladenine, BA, 0.22 μM) dosage was enough to obtain a good multiplication rate, also depending by the PC color. Rooting of proliferated shoots occurred easily in hormone-free medium and acclimatization was successfully reached in 6 weeks. Somatic embryos were formed from embryogenic callus induced by taproot sections placed on 2,4-dichlorophenoxyacetic acid (2,4-D)-added medium. After in vitro conversion in hormone-free medium, somatic embryos were also successfully acclimatized. Both microplants and somatic embryos produced true-to-type taproots (multicolored carrots), demonstrating the suitability of in vitro techniques for biodiversity conservation purposes. |
| first_indexed | 2024-03-09T16:21:40Z |
| format | Article |
| id | doaj.art-5f5b7fc65d7d4234a27471e7cea2ce26 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T16:21:40Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-5f5b7fc65d7d4234a27471e7cea2ce262023-11-24T15:15:26ZengMDPI AGHorticulturae2311-75242022-12-01812115010.3390/horticulturae8121150In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation PurposesClaudia Ruta0Angela Campanelli1Giuseppe De Mastro2Federica Blando3Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70125 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70125 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), 73100 Lecce, ItalyCarrots are a multi-purpose vegetable; hence, they have become a very popular consumers’ choice, also thanks to their nutritional value with health benefits. Several varieties and landraces exist of carrot (<i>Daucus carota</i> L. ssp. <i>sativus</i>). In Apulia (Italy), for example, a multicolored carrot landrace is present, the Polignano carrot (PC), threatened by the risk of genetic erosion. With the purpose of verifying the possibility of using in vitro culture for ex situ conservation purposes of PC, a rapid and reliable protocol for micropropagation and a highly efficient somatic embryogenesis protocol have been set up. For micropropagation studies, axillary buds from taproot were used as initial explants. Supplementation on basal medium (BM, a Murashige–Skoog modified medium) with low cytokinine (6-benzyladenine, BA, 0.22 μM) dosage was enough to obtain a good multiplication rate, also depending by the PC color. Rooting of proliferated shoots occurred easily in hormone-free medium and acclimatization was successfully reached in 6 weeks. Somatic embryos were formed from embryogenic callus induced by taproot sections placed on 2,4-dichlorophenoxyacetic acid (2,4-D)-added medium. After in vitro conversion in hormone-free medium, somatic embryos were also successfully acclimatized. Both microplants and somatic embryos produced true-to-type taproots (multicolored carrots), demonstrating the suitability of in vitro techniques for biodiversity conservation purposes.https://www.mdpi.com/2311-7524/8/12/1150carrotin vitro culturesomatic embryosendangered speciesex situ conservation |
| spellingShingle | Claudia Ruta Angela Campanelli Giuseppe De Mastro Federica Blando In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes Horticulturae carrot in vitro culture somatic embryos endangered species ex situ conservation |
| title | In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes |
| title_full | In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes |
| title_fullStr | In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes |
| title_full_unstemmed | In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes |
| title_short | In Vitro Propagation by Axillary Shoot Culture and Somatic Embryogenesis of <i>Daucus carota</i> L. subsp. <i>sativus</i>, ‘Polignano’ Landrace, for Biodiversity Conservation Purposes |
| title_sort | in vitro propagation by axillary shoot culture and somatic embryogenesis of i daucus carota i l subsp i sativus i polignano landrace for biodiversity conservation purposes |
| topic | carrot in vitro culture somatic embryos endangered species ex situ conservation |
| url | https://www.mdpi.com/2311-7524/8/12/1150 |
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