Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms
<p><span class="fontstyle0">Little is known about the antibacterial activity of licorice root extract. </span><span class="fontstyle2"><strong>Objective</strong>: </span><span class="fontstyle0">To investigate the antimicr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Faculty of Dentistry, Universitas Indonesia
2017-12-01
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| Series: | Journal of Dentistry Indonesia |
| Subjects: | |
| Online Access: | http://jdentistry.ui.ac.id/index.php/JDI/article/view/1075 |
| _version_ | 1818149524962541568 |
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| author | Suttipalin Suwannakul Plykaeow Chaibenjawong |
| author_facet | Suttipalin Suwannakul Plykaeow Chaibenjawong |
| author_sort | Suttipalin Suwannakul |
| collection | DOAJ |
| description | <p><span class="fontstyle0">Little is known about the antibacterial activity of licorice root extract. </span><span class="fontstyle2"><strong>Objective</strong>: </span><span class="fontstyle0">To investigate the antimicrobial and anti-proteolytic activities of root extract on </span><span class="fontstyle3">Porphyromonas gingivalis </span><span class="fontstyle0">in both planktonics and bioflm cells. </span><span class="fontstyle2"><strong>Methods</strong>: </span><span class="fontstyle3">Glycyrrhiza glabra </span><span class="fontstyle0">(</span><span class="fontstyle3">G. glabra</span><span class="fontstyle0">) roots were extracted by 95% ethanol freeze dried and kept at -20˚C prior experiments. </span><span class="fontstyle3">P.gingvalis </span><span class="fontstyle0">(ATCC 33277) were cultured and used for experiments. Determination of antibacterial activities of </span><span class="fontstyle3">G.glabra </span><span class="fontstyle0">extracts (lico rice) against </span><span class="fontstyle3">P.gingvalis </span><span class="fontstyle0">planktonic the MIC and MBC were evaluated by agar well diffusion, broth microdilution, and time-killing methods. The crystal violet assay was used to assess the bioflm growth inhibition and the disruption of established bioflm. The Arg - specifc proteolytic activities were analyzed using the chromogenic substrates assays using N-benzoyl-DL-arginine-4-nitroanilide hydrochloride and N-(p-tosyl)-Gly Pro-Lys 4-nitroanilide acetate salt to assess the enzymatic inhibition effects of the extracts compared with the controls. </span><span class="fontstyle2"><strong>Results</strong>: </span><span class="fontstyle0">The licorice root extract had antimicrobial activities on </span><span class="fontstyle3">P.gingivalis </span><span class="fontstyle0">with MIC and MBC of 62.5µg/ml and 25 µg/ml respectively. The assay showed that Licorice root extronidazole. Licorice root extract also had effect on </span><span class="fontstyle3">P.gingivalis </span><span class="fontstyle0">bioflms. Quantifcation by crystal violate staining showed the reduction of bioflm mass in the presence of Licorice root extract. The Arg-and Kgp- proteases activities were also inhibited by the extract in dose dependent manner. </span><span class="fontstyle2"><strong>Conclusion</strong>: </span><span class="fontstyle0">The results suggested that licorice root extract may has potential therapeutics values as a candidate for periodontal disease</span> <br /><br /></p> |
| first_indexed | 2024-12-11T13:08:25Z |
| format | Article |
| id | doaj.art-5f5c9bc93716442dba329d0a4f4b003d |
| institution | Directory Open Access Journal |
| issn | 1693-9697 2355-4800 |
| language | English |
| last_indexed | 2024-12-11T13:08:25Z |
| publishDate | 2017-12-01 |
| publisher | Faculty of Dentistry, Universitas Indonesia |
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| series | Journal of Dentistry Indonesia |
| spelling | doaj.art-5f5c9bc93716442dba329d0a4f4b003d2022-12-22T01:06:15ZengFaculty of Dentistry, Universitas IndonesiaJournal of Dentistry Indonesia1693-96972355-48002017-12-01243859210.14693/jdi.v24i3.1075888Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and BiofilmsSuttipalin Suwannakul0Plykaeow Chaibenjawong1Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Thailand<p><span class="fontstyle0">Little is known about the antibacterial activity of licorice root extract. </span><span class="fontstyle2"><strong>Objective</strong>: </span><span class="fontstyle0">To investigate the antimicrobial and anti-proteolytic activities of root extract on </span><span class="fontstyle3">Porphyromonas gingivalis </span><span class="fontstyle0">in both planktonics and bioflm cells. </span><span class="fontstyle2"><strong>Methods</strong>: </span><span class="fontstyle3">Glycyrrhiza glabra </span><span class="fontstyle0">(</span><span class="fontstyle3">G. glabra</span><span class="fontstyle0">) roots were extracted by 95% ethanol freeze dried and kept at -20˚C prior experiments. </span><span class="fontstyle3">P.gingvalis </span><span class="fontstyle0">(ATCC 33277) were cultured and used for experiments. Determination of antibacterial activities of </span><span class="fontstyle3">G.glabra </span><span class="fontstyle0">extracts (lico rice) against </span><span class="fontstyle3">P.gingvalis </span><span class="fontstyle0">planktonic the MIC and MBC were evaluated by agar well diffusion, broth microdilution, and time-killing methods. The crystal violet assay was used to assess the bioflm growth inhibition and the disruption of established bioflm. The Arg - specifc proteolytic activities were analyzed using the chromogenic substrates assays using N-benzoyl-DL-arginine-4-nitroanilide hydrochloride and N-(p-tosyl)-Gly Pro-Lys 4-nitroanilide acetate salt to assess the enzymatic inhibition effects of the extracts compared with the controls. </span><span class="fontstyle2"><strong>Results</strong>: </span><span class="fontstyle0">The licorice root extract had antimicrobial activities on </span><span class="fontstyle3">P.gingivalis </span><span class="fontstyle0">with MIC and MBC of 62.5µg/ml and 25 µg/ml respectively. The assay showed that Licorice root extronidazole. Licorice root extract also had effect on </span><span class="fontstyle3">P.gingivalis </span><span class="fontstyle0">bioflms. Quantifcation by crystal violate staining showed the reduction of bioflm mass in the presence of Licorice root extract. The Arg-and Kgp- proteases activities were also inhibited by the extract in dose dependent manner. </span><span class="fontstyle2"><strong>Conclusion</strong>: </span><span class="fontstyle0">The results suggested that licorice root extract may has potential therapeutics values as a candidate for periodontal disease</span> <br /><br /></p>http://jdentistry.ui.ac.id/index.php/JDI/article/view/1075antimicrobialbiofilmlicorice root extractPorphyromonas gingivalisproteases activities |
| spellingShingle | Suttipalin Suwannakul Plykaeow Chaibenjawong Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms Journal of Dentistry Indonesia antimicrobial biofilm licorice root extract Porphyromonas gingivalis proteases activities |
| title | Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms |
| title_full | Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms |
| title_fullStr | Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms |
| title_full_unstemmed | Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms |
| title_short | Antibacterial Activities of Glycyrrhiza gabra Linn. (Licorice) Root Extract against Porphyromonas gingivalis rand Its Inhibitory Effects on Cysteine Proteases and Biofilms |
| title_sort | antibacterial activities of glycyrrhiza gabra linn licorice root extract against porphyromonas gingivalis rand its inhibitory effects on cysteine proteases and biofilms |
| topic | antimicrobial biofilm licorice root extract Porphyromonas gingivalis proteases activities |
| url | http://jdentistry.ui.ac.id/index.php/JDI/article/view/1075 |
| work_keys_str_mv | AT suttipalinsuwannakul antibacterialactivitiesofglycyrrhizagabralinnlicoricerootextractagainstporphyromonasgingivalisranditsinhibitoryeffectsoncysteineproteasesandbiofilms AT plykaeowchaibenjawong antibacterialactivitiesofglycyrrhizagabralinnlicoricerootextractagainstporphyromonasgingivalisranditsinhibitoryeffectsoncysteineproteasesandbiofilms |