A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin
Pasteurellaceae family members obtain iron directly from host proteins or through siderophore-dependent mechanisms. Although Gallibacterum anatis expresses different virulence factors, its response to growth under iron restriction is unknown. G. anatis cultured in the presence of 2,2′-dipyridyl, up-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.951173/full |
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| author | Alberto Chantes-Guerra Samantha Maldonado-Puga Norma Rojas-Ruiz Ismael Rea-Hernandez Fernando J. Montes-Garcia Hector Trujillo-Ruiz Ivan E. Yañez-Aguilar Candelario Vazquez-Cruz Patricia Sanchez-Alonso Erasmo Negrete-Abascal |
| author_facet | Alberto Chantes-Guerra Samantha Maldonado-Puga Norma Rojas-Ruiz Ismael Rea-Hernandez Fernando J. Montes-Garcia Hector Trujillo-Ruiz Ivan E. Yañez-Aguilar Candelario Vazquez-Cruz Patricia Sanchez-Alonso Erasmo Negrete-Abascal |
| author_sort | Alberto Chantes-Guerra |
| collection | DOAJ |
| description | Pasteurellaceae family members obtain iron directly from host proteins or through siderophore-dependent mechanisms. Although Gallibacterum anatis expresses different virulence factors, its response to growth under iron restriction is unknown. G. anatis cultured in the presence of 2,2′-dipyridyl, up-expressed an approximately 65 kDa protein and repressed the expression of a 70 kDa protein. MALDI-TOF analysis of those proteins indicated homology with CirA (65 kDa), a protein involved in iron-siderophore acquisition in Mannheimia succinoproducens and a TonB-dependent receptor (70 kDa protein), a protein that binds chicken hemoglobin; however, G. anatis siderophore production was not detected by chromo azurol S (CAS)-BHI agar determination. This putative G. anatis siderophore receptor is under Fur control, but not the hemoglobin binding protein, as observed in G. anatis 12656-12 fur mutant (Ω fur 126.13) grown in the presence or not of 2,2′-dipyridyl. The addition of FeCl3 to the culture medium diminished the growth and biofilm production in approximately 30% and 35%, respectively, in the wild-type strain, but the growth of Ω fur 126.13 strain was not affected and biofilm production increased in 35%. G. anatis Ω fur 126.13 presented lower virulence when it was inoculated to 35-day-old chickens in comparison to the wild-type strain. The induction of more than one iron uptake mechanism could benefit pathogenic microorganisms such as Gallibacterium. |
| first_indexed | 2024-04-13T13:16:09Z |
| format | Article |
| id | doaj.art-5f5ce3b78996406c8b1be60ea10f06b0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T13:16:09Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5f5ce3b78996406c8b1be60ea10f06b02022-12-22T02:45:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.951173951173A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobinAlberto Chantes-Guerra0Samantha Maldonado-Puga1Norma Rojas-Ruiz2Ismael Rea-Hernandez3Fernando J. Montes-Garcia4Hector Trujillo-Ruiz5Ivan E. Yañez-Aguilar6Candelario Vazquez-Cruz7Patricia Sanchez-Alonso8Erasmo Negrete-Abascal9Instituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, MexicoInstituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, MexicoInstituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, MexicoCentro de Investigaciones y Estudios Avanzados, Instituto Politécnico Nacional de México (CINVESTAV), Mexico City, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autonóma de Mexico, Los Reyes Iztacala, Tlalnepantla de Baz, Edo de México, MexicoCientíficos Veterinarios de México, Tepatitlán, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autonóma de Mexico, Los Reyes Iztacala, Tlalnepantla de Baz, Edo de México, MexicoInstituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, MexicoInstituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autonóma de Mexico, Los Reyes Iztacala, Tlalnepantla de Baz, Edo de México, MexicoPasteurellaceae family members obtain iron directly from host proteins or through siderophore-dependent mechanisms. Although Gallibacterum anatis expresses different virulence factors, its response to growth under iron restriction is unknown. G. anatis cultured in the presence of 2,2′-dipyridyl, up-expressed an approximately 65 kDa protein and repressed the expression of a 70 kDa protein. MALDI-TOF analysis of those proteins indicated homology with CirA (65 kDa), a protein involved in iron-siderophore acquisition in Mannheimia succinoproducens and a TonB-dependent receptor (70 kDa protein), a protein that binds chicken hemoglobin; however, G. anatis siderophore production was not detected by chromo azurol S (CAS)-BHI agar determination. This putative G. anatis siderophore receptor is under Fur control, but not the hemoglobin binding protein, as observed in G. anatis 12656-12 fur mutant (Ω fur 126.13) grown in the presence or not of 2,2′-dipyridyl. The addition of FeCl3 to the culture medium diminished the growth and biofilm production in approximately 30% and 35%, respectively, in the wild-type strain, but the growth of Ω fur 126.13 strain was not affected and biofilm production increased in 35%. G. anatis Ω fur 126.13 presented lower virulence when it was inoculated to 35-day-old chickens in comparison to the wild-type strain. The induction of more than one iron uptake mechanism could benefit pathogenic microorganisms such as Gallibacterium.https://www.frontiersin.org/articles/10.3389/fmicb.2022.951173/fullGallibacteriumFuriron restrictionG. anatis fur-mutantbiofilmvirulence |
| spellingShingle | Alberto Chantes-Guerra Samantha Maldonado-Puga Norma Rojas-Ruiz Ismael Rea-Hernandez Fernando J. Montes-Garcia Hector Trujillo-Ruiz Ivan E. Yañez-Aguilar Candelario Vazquez-Cruz Patricia Sanchez-Alonso Erasmo Negrete-Abascal A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin Frontiers in Microbiology Gallibacterium Fur iron restriction G. anatis fur-mutant biofilm virulence |
| title | A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin |
| title_full | A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin |
| title_fullStr | A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin |
| title_full_unstemmed | A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin |
| title_short | A putative siderophore receptor of Gallibacterium anatis 12656-12 under Fur control also binds hemoglobin |
| title_sort | putative siderophore receptor of gallibacterium anatis 12656 12 under fur control also binds hemoglobin |
| topic | Gallibacterium Fur iron restriction G. anatis fur-mutant biofilm virulence |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.951173/full |
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