Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs
The relationship between m6A-related lncRNAs and prognosis in hepatocellular carcinoma (HCC) is not yet clear. We used Lasso regression to establish a prognostic signature based on m6A-related lncRNAs using a training set from TCGA, and then verified the signature efficacy in a test set. Fluorescenc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.691372/full |
| _version_ | 1819078842296303616 |
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| author | Ting Guo Kun He Yifei Wang Jingjing Sun Yong Chen Zelong Yang |
| author_facet | Ting Guo Kun He Yifei Wang Jingjing Sun Yong Chen Zelong Yang |
| author_sort | Ting Guo |
| collection | DOAJ |
| description | The relationship between m6A-related lncRNAs and prognosis in hepatocellular carcinoma (HCC) is not yet clear. We used Lasso regression to establish a prognostic signature based on m6A-related lncRNAs using a training set from TCGA, and then verified the signature efficacy in a test set. Fluorescence quantitative real-time PCR (qPCR), Survival analysis, clinical risk difference analysis, immune-related analysis, and drug-sensitivity analysis were conducted. The results revealed that 1,651 lncRNAs were differentially expressed in HCC tissues, among which, 163 were m6A-related. Univariate analysis showed that 87 lncRNAs were associated with the overall survival. Six differential m6A-related lncRNAs were validated and selected via Lasso regression to construct a prognostic signature which demonstrated a satisfactory predictive efficacy. In the clinically relevant pathologic stage, histologic grade, and T stage, the risk scores obtained based on this signature showed a statistically significant difference. The high- and low-risk groups exhibited a difference in the tumor immune infiltrating cells, immune checkpoint gene expression, and sensitivity to chemotherapy. In summary, the prognostic signature based on the m6A-related lncRNAs can effectively predict the prognosis of patients and might provide a new vista for the chemotherapy and immunotherapy of HCC. |
| first_indexed | 2024-12-21T19:19:31Z |
| format | Article |
| id | doaj.art-5f5eaa35b1794dff814892ac2fa7274b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-21T19:19:31Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-5f5eaa35b1794dff814892ac2fa7274b2022-12-21T18:52:58ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-08-011110.3389/fonc.2021.691372691372Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAsTing Guo0Kun He1Yifei Wang2Jingjing Sun3Yong Chen4Zelong Yang5Department of Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, ChinaDepartment of Hepatobiliary Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Hepatobiliary Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Hepatobiliary Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Hepatobiliary Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Hepatobiliary Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi’an, ChinaThe relationship between m6A-related lncRNAs and prognosis in hepatocellular carcinoma (HCC) is not yet clear. We used Lasso regression to establish a prognostic signature based on m6A-related lncRNAs using a training set from TCGA, and then verified the signature efficacy in a test set. Fluorescence quantitative real-time PCR (qPCR), Survival analysis, clinical risk difference analysis, immune-related analysis, and drug-sensitivity analysis were conducted. The results revealed that 1,651 lncRNAs were differentially expressed in HCC tissues, among which, 163 were m6A-related. Univariate analysis showed that 87 lncRNAs were associated with the overall survival. Six differential m6A-related lncRNAs were validated and selected via Lasso regression to construct a prognostic signature which demonstrated a satisfactory predictive efficacy. In the clinically relevant pathologic stage, histologic grade, and T stage, the risk scores obtained based on this signature showed a statistically significant difference. The high- and low-risk groups exhibited a difference in the tumor immune infiltrating cells, immune checkpoint gene expression, and sensitivity to chemotherapy. In summary, the prognostic signature based on the m6A-related lncRNAs can effectively predict the prognosis of patients and might provide a new vista for the chemotherapy and immunotherapy of HCC.https://www.frontiersin.org/articles/10.3389/fonc.2021.691372/fullhepatocellular carcinomalong noncoding RNAprognostic signatureimmunotherapym6A |
| spellingShingle | Ting Guo Kun He Yifei Wang Jingjing Sun Yong Chen Zelong Yang Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs Frontiers in Oncology hepatocellular carcinoma long noncoding RNA prognostic signature immunotherapy m6A |
| title | Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs |
| title_full | Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs |
| title_fullStr | Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs |
| title_full_unstemmed | Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs |
| title_short | Prognostic Signature of Hepatocellular Carcinoma and Analysis of Immune Infiltration Based on m6A-Related lncRNAs |
| title_sort | prognostic signature of hepatocellular carcinoma and analysis of immune infiltration based on m6a related lncrnas |
| topic | hepatocellular carcinoma long noncoding RNA prognostic signature immunotherapy m6A |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.691372/full |
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