| Summary: | In this study, plants derived from Helianthus annuus L. (sunflower) seeds by using
the embryo culture method were studied to preserve them at low temprature and to
determine the anatomical and karyological changes. The germinated control plants
were grown in the sand. The experimental group were cultured on MS embryo culture
medium containing, 0,5 mg/l BAP and 0,1 mg/l NAA. One-half of these plants were
kept at 16/8 hours photoperiod at 25 o
C, and the remains kept in the dark at 4 o
C. In
the anatomical evaluation, structures such as epidermis, cortex parenchyma,
parenchyma, vascular bundles of experimental group plants showed some major
differences than the control. Mitotic index values have been identified 22,9% in the
control, 60% in the experimental group grown at photoperiod at 25 o
C, 10,1% in plants
treated with dark and chilling. All of these abnormalities disappeared at normal culture
conditions and plants showed healthy development. In conclusion, it is recommended
that chilling storage of sunflower is a utilizable method.
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