ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE
In this study, plants derived from Helianthus annuus L. (sunflower) seeds by using the embryo culture method were studied to preserve them at low temprature and to determine the anatomical and karyological changes. The germinated control plants were grown in the sand. The experimental group were cul...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Trakya University
2016-11-01
|
| Series: | Trakya University Journal of Natural Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/234515 |
| _version_ | 1797633869365641216 |
|---|---|
| author | Hayati Arda Sergun Dayan Gökçe Akpınar |
| author_facet | Hayati Arda Sergun Dayan Gökçe Akpınar |
| author_sort | Hayati Arda |
| collection | DOAJ |
| description | In this study, plants derived from Helianthus annuus L. (sunflower) seeds by using
the embryo culture method were studied to preserve them at low temprature and to
determine the anatomical and karyological changes. The germinated control plants
were grown in the sand. The experimental group were cultured on MS embryo culture
medium containing, 0,5 mg/l BAP and 0,1 mg/l NAA. One-half of these plants were
kept at 16/8 hours photoperiod at 25 o
C, and the remains kept in the dark at 4 o
C. In
the anatomical evaluation, structures such as epidermis, cortex parenchyma,
parenchyma, vascular bundles of experimental group plants showed some major
differences than the control. Mitotic index values have been identified 22,9% in the
control, 60% in the experimental group grown at photoperiod at 25 o
C, 10,1% in plants
treated with dark and chilling. All of these abnormalities disappeared at normal culture
conditions and plants showed healthy development. In conclusion, it is recommended
that chilling storage of sunflower is a utilizable method. |
| first_indexed | 2024-03-11T11:59:37Z |
| format | Article |
| id | doaj.art-5f5ef32097524bef9719b5aa6654ee81 |
| institution | Directory Open Access Journal |
| issn | 2528-9691 |
| language | English |
| last_indexed | 2024-03-11T11:59:37Z |
| publishDate | 2016-11-01 |
| publisher | Trakya University |
| record_format | Article |
| series | Trakya University Journal of Natural Sciences |
| spelling | doaj.art-5f5ef32097524bef9719b5aa6654ee812023-11-08T07:27:57ZengTrakya UniversityTrakya University Journal of Natural Sciences2528-96912016-11-01132758669ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATUREHayati ArdaSergun DayanGökçe AkpınarIn this study, plants derived from Helianthus annuus L. (sunflower) seeds by using the embryo culture method were studied to preserve them at low temprature and to determine the anatomical and karyological changes. The germinated control plants were grown in the sand. The experimental group were cultured on MS embryo culture medium containing, 0,5 mg/l BAP and 0,1 mg/l NAA. One-half of these plants were kept at 16/8 hours photoperiod at 25 o C, and the remains kept in the dark at 4 o C. In the anatomical evaluation, structures such as epidermis, cortex parenchyma, parenchyma, vascular bundles of experimental group plants showed some major differences than the control. Mitotic index values have been identified 22,9% in the control, 60% in the experimental group grown at photoperiod at 25 o C, 10,1% in plants treated with dark and chilling. All of these abnormalities disappeared at normal culture conditions and plants showed healthy development. In conclusion, it is recommended that chilling storage of sunflower is a utilizable method.https://dergipark.org.tr/en/download/article-file/234515embriyo kültürüanatomimitotik indeksayçiçeği.embryo cultureanatomymitotic indexsunflower |
| spellingShingle | Hayati Arda Sergun Dayan Gökçe Akpınar ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE Trakya University Journal of Natural Sciences embriyo kültürü anatomi mitotik indeks ayçiçeği. embryo culture anatomy mitotic index sunflower |
| title | ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE |
| title_full | ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE |
| title_fullStr | ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE |
| title_full_unstemmed | ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE |
| title_short | ANATOMICAL AND CARYOLOGICAL INVESTIGATIONS ON in vitro SUNFLOWER (Helianthus annuus L.) PLANTS PRESERVED AT LOW TEMPRATURE |
| title_sort | anatomical and caryological investigations on in vitro sunflower helianthus annuus l plants preserved at low temprature |
| topic | embriyo kültürü anatomi mitotik indeks ayçiçeği. embryo culture anatomy mitotic index sunflower |
| url | https://dergipark.org.tr/en/download/article-file/234515 |
| work_keys_str_mv | AT hayatiarda anatomicalandcaryologicalinvestigationsoninvitrosunflowerhelianthusannuuslplantspreservedatlowtemprature AT sergundayan anatomicalandcaryologicalinvestigationsoninvitrosunflowerhelianthusannuuslplantspreservedatlowtemprature AT gokceakpınar anatomicalandcaryologicalinvestigationsoninvitrosunflowerhelianthusannuuslplantspreservedatlowtemprature |