Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats
Abstract Fibroblast growth factor 23, parathyroid hormone, and 1,25-dihydroxyvitamin D are critical in phosphate homeostasis. Despite these factors’ importance, regulators of phosphaturia in the acute postprandial phase remain largely unknown. This study investigated the mechanism of acute phosphate...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32856-2 |
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| author | Seiichi Yasuda Kazunori Inoue Isao Matsui Ayumi Matsumoto Yusuke Katsuma Hiroki Okushima Atsuhiro Imai Yusuke Sakaguchi Jun-ya Kaimori Ryohei Yamamoto Masayuki Mizui Yoshitaka Isaka |
| author_facet | Seiichi Yasuda Kazunori Inoue Isao Matsui Ayumi Matsumoto Yusuke Katsuma Hiroki Okushima Atsuhiro Imai Yusuke Sakaguchi Jun-ya Kaimori Ryohei Yamamoto Masayuki Mizui Yoshitaka Isaka |
| author_sort | Seiichi Yasuda |
| collection | DOAJ |
| description | Abstract Fibroblast growth factor 23, parathyroid hormone, and 1,25-dihydroxyvitamin D are critical in phosphate homeostasis. Despite these factors’ importance, regulators of phosphaturia in the acute postprandial phase remain largely unknown. This study investigated the mechanism of acute phosphate regulation in the postprandial phase in rats. Duodenal administration of radiolabeled phosphate (32P) showed that 32P levels in the inferior vena cava (IVC) blood were lower than those in the portal vein (PV) blood. Serum phosphate concentration transiently increased 5 min after phosphate solution administration through IVC, while it was maintained after the administration through PV. Phosphate administration through both IVC and PV resulted in increased fractional excretion of phosphate (FEPi) at 10 min without elevation of the known circulating factors, but urinary phosphate excretion during the period was 8% of the dose. Experiments using 32P or partial hepatectomy showed that the liver was one of the phosphate reservoirs. The elevation of FEPi and suppression of sodium-phosphate cotransporter 2a in the kidney at 10 min was attenuated in rats with SCH23390, hepatic denervation, or renal denervation, thus indicating that the liver communicated with the kidney via the nervous system to promote phosphaturia. These results revealed previously unknown mechanisms for serum phosphate maintenance. |
| first_indexed | 2024-04-09T18:53:48Z |
| format | Article |
| id | doaj.art-5f63537a00b54eb08d6fb8709bd33320 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T18:53:48Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5f63537a00b54eb08d6fb8709bd333202023-04-09T11:17:05ZengNature PortfolioScientific Reports2045-23222023-04-0113111510.1038/s41598-023-32856-2Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in ratsSeiichi Yasuda0Kazunori Inoue1Isao Matsui2Ayumi Matsumoto3Yusuke Katsuma4Hiroki Okushima5Atsuhiro Imai6Yusuke Sakaguchi7Jun-ya Kaimori8Ryohei Yamamoto9Masayuki Mizui10Yoshitaka Isaka11Department of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Inter-Organ Communication Research in Kidney Disease, Osaka University Graduate School of MedicineDepartment of Inter-Organ Communication Research in Kidney Disease, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineDepartment of Nephrology, Osaka University Graduate School of MedicineAbstract Fibroblast growth factor 23, parathyroid hormone, and 1,25-dihydroxyvitamin D are critical in phosphate homeostasis. Despite these factors’ importance, regulators of phosphaturia in the acute postprandial phase remain largely unknown. This study investigated the mechanism of acute phosphate regulation in the postprandial phase in rats. Duodenal administration of radiolabeled phosphate (32P) showed that 32P levels in the inferior vena cava (IVC) blood were lower than those in the portal vein (PV) blood. Serum phosphate concentration transiently increased 5 min after phosphate solution administration through IVC, while it was maintained after the administration through PV. Phosphate administration through both IVC and PV resulted in increased fractional excretion of phosphate (FEPi) at 10 min without elevation of the known circulating factors, but urinary phosphate excretion during the period was 8% of the dose. Experiments using 32P or partial hepatectomy showed that the liver was one of the phosphate reservoirs. The elevation of FEPi and suppression of sodium-phosphate cotransporter 2a in the kidney at 10 min was attenuated in rats with SCH23390, hepatic denervation, or renal denervation, thus indicating that the liver communicated with the kidney via the nervous system to promote phosphaturia. These results revealed previously unknown mechanisms for serum phosphate maintenance.https://doi.org/10.1038/s41598-023-32856-2 |
| spellingShingle | Seiichi Yasuda Kazunori Inoue Isao Matsui Ayumi Matsumoto Yusuke Katsuma Hiroki Okushima Atsuhiro Imai Yusuke Sakaguchi Jun-ya Kaimori Ryohei Yamamoto Masayuki Mizui Yoshitaka Isaka Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats Scientific Reports |
| title | Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| title_full | Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| title_fullStr | Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| title_full_unstemmed | Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| title_short | Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| title_sort | hepatic phosphate uptake and subsequent nerve mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats |
| url | https://doi.org/10.1038/s41598-023-32856-2 |
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