Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana
Abstract Cesium (Cs) is found at low levels in nature but does not confer any known benefit to plants. Cs and K compete in cells due to the chemical similarity of Cs to potassium (K), and can induce K deficiency in cells. In previous studies, we identified chemicals that increase Cs tolerance in pla...
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-00564-4 |
| _version_ | 1819024890026524672 |
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| author | Ju Yeon Moon Eri Adams Takae Miyazaki Yasumitsu Kondoh Makoto Muroi Nobumoto Watanabe Hiroyuki Osada Ryoung Shin |
| author_facet | Ju Yeon Moon Eri Adams Takae Miyazaki Yasumitsu Kondoh Makoto Muroi Nobumoto Watanabe Hiroyuki Osada Ryoung Shin |
| author_sort | Ju Yeon Moon |
| collection | DOAJ |
| description | Abstract Cesium (Cs) is found at low levels in nature but does not confer any known benefit to plants. Cs and K compete in cells due to the chemical similarity of Cs to potassium (K), and can induce K deficiency in cells. In previous studies, we identified chemicals that increase Cs tolerance in plants. Among them, a small chemical compound (C17H19F3N2O2), named CsToAcE1, was confirmed to enhance Cs tolerance while increasing Cs accumulation in plants. Treatment of plants with CsToAcE1 resulted in greater Cs and K accumulation and also alleviated Cs-induced growth retardation in Arabidopsis. In the present study, potential target proteins of CsToAcE1 were isolated from Arabidopsis to determine the mechanism by which CsToAcE1 alleviates Cs stress, while enhancing Cs accumulation. Our analysis identified one of the interacting target proteins of CsToAcE1 to be BETA-GLUCOSIDASE 23 (AtβGLU23). Interestingly, Arabidopsis atβglu23 mutants exhibited enhanced tolerance to Cs stress but did not respond to the application of CsToAcE1. Notably, application of CsToAcE1 resulted in a reduction of Cs-induced AtβGLU23 expression in wild-type plants, while this was not observed in a high affinity transporter mutant, athak5. Our data indicate that AtβGLU23 regulates plant response to Cs stress and that CsToAcE1 enhances Cs tolerance by repressing AtβGLU23. In addition, AtHAK5 also appears to be involved in this response. |
| first_indexed | 2024-12-21T05:01:58Z |
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| id | doaj.art-5f63f537b6cd4b178e121c548209c675 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T05:01:58Z |
| publishDate | 2021-10-01 |
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| series | Scientific Reports |
| spelling | doaj.art-5f63f537b6cd4b178e121c548209c6752022-12-21T19:15:13ZengNature PortfolioScientific Reports2045-23222021-10-0111111110.1038/s41598-021-00564-4Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thalianaJu Yeon Moon0Eri Adams1Takae Miyazaki2Yasumitsu Kondoh3Makoto Muroi4Nobumoto Watanabe5Hiroyuki Osada6Ryoung Shin7Environmental Response Research Unit, RIKEN Center for Sustainable Resource ScienceEnvironmental Response Research Unit, RIKEN Center for Sustainable Resource ScienceEnvironmental Response Research Unit, RIKEN Center for Sustainable Resource ScienceChemical Biology Research Group, RIKEN Center for Sustainable Resource ScienceChemical Biology Research Group, RIKEN Center for Sustainable Resource ScienceBioprobe Application Research Unit, RIKEN Center for Sustainable Resource ScienceChemical Biology Research Group, RIKEN Center for Sustainable Resource ScienceEnvironmental Response Research Unit, RIKEN Center for Sustainable Resource ScienceAbstract Cesium (Cs) is found at low levels in nature but does not confer any known benefit to plants. Cs and K compete in cells due to the chemical similarity of Cs to potassium (K), and can induce K deficiency in cells. In previous studies, we identified chemicals that increase Cs tolerance in plants. Among them, a small chemical compound (C17H19F3N2O2), named CsToAcE1, was confirmed to enhance Cs tolerance while increasing Cs accumulation in plants. Treatment of plants with CsToAcE1 resulted in greater Cs and K accumulation and also alleviated Cs-induced growth retardation in Arabidopsis. In the present study, potential target proteins of CsToAcE1 were isolated from Arabidopsis to determine the mechanism by which CsToAcE1 alleviates Cs stress, while enhancing Cs accumulation. Our analysis identified one of the interacting target proteins of CsToAcE1 to be BETA-GLUCOSIDASE 23 (AtβGLU23). Interestingly, Arabidopsis atβglu23 mutants exhibited enhanced tolerance to Cs stress but did not respond to the application of CsToAcE1. Notably, application of CsToAcE1 resulted in a reduction of Cs-induced AtβGLU23 expression in wild-type plants, while this was not observed in a high affinity transporter mutant, athak5. Our data indicate that AtβGLU23 regulates plant response to Cs stress and that CsToAcE1 enhances Cs tolerance by repressing AtβGLU23. In addition, AtHAK5 also appears to be involved in this response.https://doi.org/10.1038/s41598-021-00564-4 |
| spellingShingle | Ju Yeon Moon Eri Adams Takae Miyazaki Yasumitsu Kondoh Makoto Muroi Nobumoto Watanabe Hiroyuki Osada Ryoung Shin Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana Scientific Reports |
| title | Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana |
| title_full | Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana |
| title_fullStr | Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana |
| title_full_unstemmed | Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana |
| title_short | Cesium tolerance is enhanced by a chemical which binds to BETA-GLUCOSIDASE 23 in Arabidopsis thaliana |
| title_sort | cesium tolerance is enhanced by a chemical which binds to beta glucosidase 23 in arabidopsis thaliana |
| url | https://doi.org/10.1038/s41598-021-00564-4 |
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