THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN
Fifty common kilka (Clupeonella cultriventris) specimens from Guilan Province and fifty others from MazandaranProvince, South Caspian Sea were collected to study genetic variation in the fish using Restricted Fragment Length Polymorphism (RFLP) of the mtDNA. DNA was extracted from fin tissue by phen...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Iranian Fisheries Research Organization
2007-01-01
|
| Series: | Majallah-i ̒Ilmī-i Shīlāt-i Īrān |
| Subjects: | |
| Online Access: | http://isfj.areo.ir/article_113988_en.html |
| _version_ | 1818385127687847936 |
|---|---|
| author | F. Laloei1; S. Rezvani2; M. Nayerani1; M. Taghavi1 |
| author_facet | F. Laloei1; S. Rezvani2; M. Nayerani1; M. Taghavi1 |
| author_sort | F. Laloei1; S. Rezvani2; M. Nayerani1; M. Taghavi1 |
| collection | DOAJ |
| description | Fifty common kilka (Clupeonella cultriventris) specimens from Guilan Province and fifty others from MazandaranProvince, South Caspian Sea were collected to study genetic variation in the fish using Restricted Fragment Length Polymorphism (RFLP) of the mtDNA. DNA was extracted from fin tissue by phenol-chloroform method. The PCR products were digested using 13 restriction endo-nuclease enzymes. Five out of thirteen restriction enzymes were polymorphic resulting in nine different haplotypes. The haplotype divergence ranged from 0.0073 to 0.0369. The mean value of haplotype and nucleotide diversity among populations was 0.7339±0.0006 and 0.0098±0.0, respectively. The nucleotide divergence among populations was 0.01%. Statistically significant differences in haplotype frequencies among all samples were observed (P<0.01). Therefore, we conclude the populations are different genetically. |
| first_indexed | 2024-12-14T03:33:13Z |
| format | Article |
| id | doaj.art-5f64d717abf0448782a6cadde7223828 |
| institution | Directory Open Access Journal |
| issn | 1026-1354 2322-5998 |
| language | fas |
| last_indexed | 2024-12-14T03:33:13Z |
| publishDate | 2007-01-01 |
| publisher | Iranian Fisheries Research Organization |
| record_format | Article |
| series | Majallah-i ̒Ilmī-i Shīlāt-i Īrān |
| spelling | doaj.art-5f64d717abf0448782a6cadde72238282022-12-21T23:18:42ZfasIranian Fisheries Research OrganizationMajallah-i ̒Ilmī-i Shīlāt-i Īrān1026-13542322-59982007-01-01152119128THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRANF. Laloei1; S. Rezvani2; M. Nayerani1; M. Taghavi1Fifty common kilka (Clupeonella cultriventris) specimens from Guilan Province and fifty others from MazandaranProvince, South Caspian Sea were collected to study genetic variation in the fish using Restricted Fragment Length Polymorphism (RFLP) of the mtDNA. DNA was extracted from fin tissue by phenol-chloroform method. The PCR products were digested using 13 restriction endo-nuclease enzymes. Five out of thirteen restriction enzymes were polymorphic resulting in nine different haplotypes. The haplotype divergence ranged from 0.0073 to 0.0369. The mean value of haplotype and nucleotide diversity among populations was 0.7339±0.0006 and 0.0098±0.0, respectively. The nucleotide divergence among populations was 0.01%. Statistically significant differences in haplotype frequencies among all samples were observed (P<0.01). Therefore, we conclude the populations are different genetically.http://isfj.areo.ir/article_113988_en.htmlKilka; CLUPEONELLA CULTRIVENTRIS; PCR- RFLP; SOUTH CASPIAN SEA; Iran |
| spellingShingle | F. Laloei1; S. Rezvani2; M. Nayerani1; M. Taghavi1 THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN Majallah-i ̒Ilmī-i Shīlāt-i Īrān Kilka; CLUPEONELLA CULTRIVENTRIS; PCR- RFLP; SOUTH CASPIAN SEA; Iran |
| title | THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN |
| title_full | THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN |
| title_fullStr | THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN |
| title_full_unstemmed | THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN |
| title_short | THE PCR-RFLP INVESTIGATION OF CLUPEONELLA CULTRIVENTRIS FROM THE SOUTH CASPIAN SEA, IRAN |
| title_sort | pcr rflp investigation of clupeonella cultriventris from the south caspian sea iran |
| topic | Kilka; CLUPEONELLA CULTRIVENTRIS; PCR- RFLP; SOUTH CASPIAN SEA; Iran |
| url | http://isfj.areo.ir/article_113988_en.html |
| work_keys_str_mv | AT flaloei1srezvani2mnayerani1mtaghavi1 thepcrrflpinvestigationofclupeonellacultriventrisfromthesouthcaspianseairan AT flaloei1srezvani2mnayerani1mtaghavi1 pcrrflpinvestigationofclupeonellacultriventrisfromthesouthcaspianseairan |