Effect of blue light on proliferation of human retinal pigment epithelial cells
AIM: To investigate the influence and mechanism of blue light on the proliferation of human retinal pigment epithelial cells. <p>METHODS: Cells were divided into two groups, including blue light group and control group. The 35W white light lamp with blue filter was used to establish damaged RP...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2017-08-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2017/8/201708007.pdf |
| _version_ | 1819208620479348736 |
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| author | Hong-Na Zhu Ying Qiao An-Le Su Ting Zhang Zhong-Yang Sun Hou-Cheng Liang |
| author_facet | Hong-Na Zhu Ying Qiao An-Le Su Ting Zhang Zhong-Yang Sun Hou-Cheng Liang |
| author_sort | Hong-Na Zhu |
| collection | DOAJ |
| description | AIM: To investigate the influence and mechanism of blue light on the proliferation of human retinal pigment epithelial cells. <p>METHODS: Cells were divided into two groups, including blue light group and control group. The 35W white light lamp with blue filter was used to establish damaged RPE cell model <i>in vitro</i>. Blue ray wavelength ranged between 470nm and 520nm. And the light intensity was about 2 000Lx. After exposure to blue light, we tested the proliferation of human retinal pigment epithelial cells by CCK-8 kit. And then expression of miR-103 was measured by the real-time PCR. <p>RESULTS: Exposure to blue light inhibited the proliferation of human retinal pigment epithelial cells and increased the expression of miR-103. Moreover, up-regulation of miR-103 inhibited the proliferation of human retinal pigment epithelial cells, and down-regulates miR-103 promoted the proliferation of human retinal pigment epithelial cells. <p>CONCLUSION: Blue light inhibits the proliferation of human retinal pigment epithelial cells by the up-regulation of miR-103. |
| first_indexed | 2024-12-23T05:42:17Z |
| format | Article |
| id | doaj.art-5f6c17f7e9ad4dca854c88590e25ac5c |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-23T05:42:17Z |
| publishDate | 2017-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-5f6c17f7e9ad4dca854c88590e25ac5c2022-12-21T17:58:09ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232017-08-011781419142210.3980/j.issn.1672-5123.2017.8.07Effect of blue light on proliferation of human retinal pigment epithelial cellsHong-Na Zhu0Ying Qiao1An-Le Su2Ting Zhang3Zhong-Yang Sun4Hou-Cheng Liang5Department of Ophthalmology, Xi'an NO. 1 Hospital, Xi'an 710002, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an NO. 1 Hospital, Xi'an 710002, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an NO. 1 Hospital, Xi'an 710002, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an NO. 1 Hospital, Xi'an 710002, Shaanxi Province, Chinathe 454 Hospital of PLA, Nanjing 221002, Jiangsu Province, ChinaDepartment of Ophthalmology, Xi'an NO. 1 Hospital, Xi'an 710002, Shaanxi Province, ChinaAIM: To investigate the influence and mechanism of blue light on the proliferation of human retinal pigment epithelial cells. <p>METHODS: Cells were divided into two groups, including blue light group and control group. The 35W white light lamp with blue filter was used to establish damaged RPE cell model <i>in vitro</i>. Blue ray wavelength ranged between 470nm and 520nm. And the light intensity was about 2 000Lx. After exposure to blue light, we tested the proliferation of human retinal pigment epithelial cells by CCK-8 kit. And then expression of miR-103 was measured by the real-time PCR. <p>RESULTS: Exposure to blue light inhibited the proliferation of human retinal pigment epithelial cells and increased the expression of miR-103. Moreover, up-regulation of miR-103 inhibited the proliferation of human retinal pigment epithelial cells, and down-regulates miR-103 promoted the proliferation of human retinal pigment epithelial cells. <p>CONCLUSION: Blue light inhibits the proliferation of human retinal pigment epithelial cells by the up-regulation of miR-103.http://ies.ijo.cn/cn_publish/2017/8/201708007.pdfblue lighthuman retinal pigment epithelial cellsproliferationmiR-103miR-103 |
| spellingShingle | Hong-Na Zhu Ying Qiao An-Le Su Ting Zhang Zhong-Yang Sun Hou-Cheng Liang Effect of blue light on proliferation of human retinal pigment epithelial cells Guoji Yanke Zazhi blue light human retinal pigment epithelial cells proliferation miR-103 miR-103 |
| title | Effect of blue light on proliferation of human retinal pigment epithelial cells |
| title_full | Effect of blue light on proliferation of human retinal pigment epithelial cells |
| title_fullStr | Effect of blue light on proliferation of human retinal pigment epithelial cells |
| title_full_unstemmed | Effect of blue light on proliferation of human retinal pigment epithelial cells |
| title_short | Effect of blue light on proliferation of human retinal pigment epithelial cells |
| title_sort | effect of blue light on proliferation of human retinal pigment epithelial cells |
| topic | blue light human retinal pigment epithelial cells proliferation miR-103 miR-103 |
| url | http://ies.ijo.cn/cn_publish/2017/8/201708007.pdf |
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