Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis
Development of artificial bone substitutes mimicking the extracellular matrix is a promising strategy for bone repair and regeneration. In views of the actual requirement of biomechanics, biodegradability, and bioactivity, herein, a double-network (DN) hydrogel was constructed by interspersing a met...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.977419/full |
| _version_ | 1811214993204445184 |
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| author | Guoke Tang Guoke Tang Liang Zhu Weiheng Wang Dongqing Zuo Changgui Shi Xiaojie Yu Rui Chen |
| author_facet | Guoke Tang Guoke Tang Liang Zhu Weiheng Wang Dongqing Zuo Changgui Shi Xiaojie Yu Rui Chen |
| author_sort | Guoke Tang |
| collection | DOAJ |
| description | Development of artificial bone substitutes mimicking the extracellular matrix is a promising strategy for bone repair and regeneration. In views of the actual requirement of biomechanics, biodegradability, and bioactivity, herein, a double-network (DN) hydrogel was constructed by interspersing a methacrylated gelatin (GelMA) network into alendronate (ALN)-modified oxidized alginate (OSA) network via Schiff base reaction and photo-crosslinking process to promote in situ bone regeneration. This GelMA@OSA-ALN DN hydrogel possessed favorable network and pores, good biocompatibility, and enhanced biomechanics. Notably, the introduction of Schiff base furnished the ND hydrogel scaffold with pH-responsive biodegradation and sustained ALN drug release delivery, which could provide effective bioactivity, upregulate osteogenesis-related genes, and promote the cell viability, growth, proliferation, and osteogenesis differentiation for bone regeneration. Therefore, we provide a new insight to develop functional DN hydrogel scaffold toward governing the on-demand drug release and achieving the stem cell therapy, which will be developed into the minimally invasive gelling system to prolong local delivery of bisphosphonates for the bone-related diseases. |
| first_indexed | 2024-04-12T06:13:44Z |
| format | Article |
| id | doaj.art-5f6c1a5087ee4ccda3be8e941244a5dc |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-12T06:13:44Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-5f6c1a5087ee4ccda3be8e941244a5dc2022-12-22T03:44:33ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-08-011010.3389/fchem.2022.977419977419Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesisGuoke Tang0Guoke Tang1Liang Zhu2Weiheng Wang3Dongqing Zuo4Changgui Shi5Xiaojie Yu6Rui Chen7Department of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Orthopedics, Hunan Aerospace Hospital, Changsha, Hunan, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, ChinaDevelopment of artificial bone substitutes mimicking the extracellular matrix is a promising strategy for bone repair and regeneration. In views of the actual requirement of biomechanics, biodegradability, and bioactivity, herein, a double-network (DN) hydrogel was constructed by interspersing a methacrylated gelatin (GelMA) network into alendronate (ALN)-modified oxidized alginate (OSA) network via Schiff base reaction and photo-crosslinking process to promote in situ bone regeneration. This GelMA@OSA-ALN DN hydrogel possessed favorable network and pores, good biocompatibility, and enhanced biomechanics. Notably, the introduction of Schiff base furnished the ND hydrogel scaffold with pH-responsive biodegradation and sustained ALN drug release delivery, which could provide effective bioactivity, upregulate osteogenesis-related genes, and promote the cell viability, growth, proliferation, and osteogenesis differentiation for bone regeneration. Therefore, we provide a new insight to develop functional DN hydrogel scaffold toward governing the on-demand drug release and achieving the stem cell therapy, which will be developed into the minimally invasive gelling system to prolong local delivery of bisphosphonates for the bone-related diseases.https://www.frontiersin.org/articles/10.3389/fchem.2022.977419/fullGelMAALNOSAschiff baseDN hydrogelosteogenic differentiation |
| spellingShingle | Guoke Tang Guoke Tang Liang Zhu Weiheng Wang Dongqing Zuo Changgui Shi Xiaojie Yu Rui Chen Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis Frontiers in Chemistry GelMA ALN OSA schiff base DN hydrogel osteogenic differentiation |
| title | Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis |
| title_full | Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis |
| title_fullStr | Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis |
| title_full_unstemmed | Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis |
| title_short | Alendronate-functionalized double network hydrogel scaffolds for effective osteogenesis |
| title_sort | alendronate functionalized double network hydrogel scaffolds for effective osteogenesis |
| topic | GelMA ALN OSA schiff base DN hydrogel osteogenic differentiation |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.977419/full |
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